Juan Carlos de la Torre
A5035095483- Viral Infections and Outbreaks Research
- Viral Infections and Vectors
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Virology and Viral Diseases
- Virus-based gene therapy research
- interferon and immune responses
- Hepatitis B Virus Studies
- Plant Virus Research Studies
- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Immunology Research
- Immune Cell Function and Interaction
- Mosquito-borne diseases and control
- Immunotherapy and Immune Responses
- Rabies epidemiology and control
- Vector-Borne Animal Diseases
- Respiratory viral infections research
- Bacillus and Francisella bacterial research
- Animal Disease Management and Epidemiology
- T-cell and B-cell Immunology
- COVID-19 Clinical Research Studies
- Plant and Fungal Interactions Research
- Parvovirus B19 Infection Studies
- SARS-CoV-2 detection and testing
- Bacteriophages and microbial interactions
Scripps Research Institute
2016-2025
Hospital Universitario Fundación Jiménez Díaz
2024
Scripps (United States)
2021-2023
Scripps Institution of Oceanography
2020-2023
Scripps Health
1990-2022
Scripps Clinic
1990-2017
Laboratoire d’immunologie intégrative du cancer
2006
Pennsylvania State University
2003
University of California, San Diego
1989-1992
Freie Universität Berlin
1992
INTERFER(ON)ing Persistence During persistent viral infections, a dysregulated immune response fails to control the infection. Wilson et al. (p. 202 ) and Teijaro 207 ; see Perspective by Odorizzi Wherry show this occurs because type I interferons (IFN I), critical for early responses infection, contribute altered immunity seen during Antibody blockade of IFN signaling chronic lymphocytic choriomeningitis virus (LCMV) in mice resulted reduced titers at later stages expression inhibitory...
Abstract Lassa fever is a severe multisystem disease that often has haemorrhagic manifestations. The epitopes of the virus (LASV) surface glycoproteins recognized by naturally infected human hosts have not been identified or characterized. Here we cloned 113 monoclonal antibodies (mAbs) specific for LASV from memory B cells survivors West Africa. One-half bind GP2 fusion subunit, one-fourth recognize GP1 receptor-binding subunit and remaining fourth are assembled glycoprotein complex,...
By using a reverse genetics system that is based on the prototypic arenavirus lymphocytic choriomeningitis virus (LCMV), we have identified small RING finger Z protein as main driving force of budding. Both LCMV and Lassa fever (LFV) proteins exhibited self-budding activity, both substituted efficiently for late domain present in Gag Rous sarcoma virus. LFV contain proline-rich motifs are characteristic domains. Mutations PPPY motif severely impaired formation virus-like particles. contains...
Among cells of the immune system, CD11c(+) and DEC-205(+) splenic dendritic primarily express cellular receptor (alpha-dystroglycan [alpha-DG]) for lymphocytic choriomeningitis virus (LCMV). By selection, strains variants LCMV that bind alpha-DG with high affinity are associated replication in white pulp, show preferential a majority cells, cause immunosuppression, establish persistent infection. In contrast, viral low to red display minimal generate robust anti-LCMV cytotoxic T lymphocyte...
We describe a sensitive, internally controlled method for comparing the genetic adaptability and relative fitness of virus populations in constant or changing host environments. Certain monoclonal antibody-resistant mutants vesicular stomatitis can compete equally during serial passages mixtures with parental wild-type clone from which they were derived. These genetically marked "surrogate wild-type" neutral mutants, when mixed virus, allow reliable measurement changes adaptation to...
We have previously demonstrated that Borna disease virus (BDV) has a negative nonsegmented single-stranded (NNS) RNA genome replicates in the nucleus of infected cells. Here we report for first time cloning and complete sequence BDV genome. Our results revealed genomic organization similar to other members Mononegavirales order. identified five main open reading frames (ORFs). The largest ORF, V, is located closest 5' end and, on basis strong homology with NNS-RNA polymerases, member...
Mutagenesis by a variety of chemical mutagens conferred only 1.1- to 2.8-fold increases in mutation frequencies at defined single base sites vesicular stomatitis virus and poliovirus.
The prototypic arenavirus lymphocytic choriomeningitis virus (LCMV) is a formidable battle horse for the study of viral immunology, as well persistence and associated diseases. Investigations with LCMV have uncovered basic mechanisms by which viruses avoid elimination host adaptive immune response. In this we show that also disables innate defense interfering beta interferon (IFN-beta) production in response to different stimuli, including infection Sendai liposome-mediated DNA transfection....
ABSTRACT The genome of lymphocytic choriomeningitis virus (LCMV) consists two negative-sense single-stranded RNA segments, designated L and S. Both segments contain viral genes in an ambisense coding strategy, with the being separated by intergenic region (IGR). We have developed a reverse genetic system that allows investigation cis -acting signals trans factors involved transcription replication LCMV. To this end, we constructed LCMV S minigenome consisting copy chloramphenicol...
Measles virus (MV), a member of the family Paramyxoviridae and an exclusively human pathogen, is among most infectious viruses. A progressive fatal neurodegenerative complication, subacute sclerosing panencephalitis (SSPE), occurs during persistent MV infection CNS associated with biased hypermutations viral genome. The observed A-to-G are consistent conversions catalyzed by adenosine deaminase acting on RNA (ADAR1). To evaluate role ADAR1 in infection, we selectively disrupted expression...
Borna disease virus, an unclassified infectious agent, causes immune-mediated neurologic in a wide variety of animal hosts and may be involved pathogenesis selected neuropsychiatric diseases man. Initial reports suggested that virus is single-stranded RNA virus. We describe here method for isolation viral particles has allowed definitive identification the genome as containing negative-polarity RNA. Further, we show mRNAs are transcribed nucleus.