A5001905405- Viral Infections and Vectors
- SARS-CoV-2 and COVID-19 Research
- Influenza Virus Research Studies
- Animal Disease Management and Epidemiology
- Animal Virus Infections Studies
- Poxvirus research and outbreaks
- Virology and Viral Diseases
- Vector-Borne Animal Diseases
- Viral gastroenteritis research and epidemiology
- Herpesvirus Infections and Treatments
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- Vector-borne infectious diseases
- Rabies epidemiology and control
- Respiratory viral infections research
- Bacillus and Francisella bacterial research
- Virus-based gene therapy research
- Bacteriophages and microbial interactions
- Microbial infections and disease research
- Zoonotic diseases and public health
- Plant Virus Research Studies
- Viral Infections and Outbreaks Research
- Mosquito-borne diseases and control
- Sperm and Testicular Function
- interferon and immune responses
Friedrich-Loeffler-Institut
2016-2025
Omron (Japan)
2023
Hebrew University of Jerusalem
2023
University of Veterinary Medicine Hannover, Foundation
2021
Ludwig-Maximilians-Universität München
2021
World Health Organization
2020
University Hospital Schleswig-Holstein
2018
University of Lübeck
2018
University Medical Center
2018
Kiel University
2018
In December, 2019, a novel zoonotic severe acute respiratory syndrome-related coronavirus emerged in China. The syndrome 2 (SARS-CoV-2) became pandemic within weeks and the number of human infections cases is increasing. We aimed to investigate susceptibilty potential animal hosts risk anthropozoonotic spill-over infections.We intranasally inoculated nine fruit bats (Rousettus aegyptiacus), ferrets (Mustela putorius), pigs (Sus scrofa domesticus), 17 chickens (Gallus gallus domesticus) with...
In late 2019, an outbreak of a severe respiratory disease caused by emerging coronavirus, SARS-CoV-2, resulted in high morbidity and mortality infected humans. Complete understanding COVID-19, the multi-faceted requires suitable small animal models, as does development evaluation vaccines antivirals. Since age-dependent differences COVID-19 were identified humans, we compared course SARS-CoV-2 infection young aged Syrian hamsters. We show that virus replication upper lower tract was...
Raccoon dogs might have been intermediate hosts for severe acute respiratory syndrome-associated coronavirus in 2002-2004. We demonstrated susceptibility of raccoon to syndrome 2 infection and transmission in-contact animals. Infected animals had no signs illness. Virus replication tissue lesions occurred the nasal conchae.
Abstract We inoculated 6 cattle with severe acute respiratory syndrome coronavirus 2 and kept them together 3 uninoculated cattle. observed viral replication specific seroreactivity in animals, despite high levels of preexisting antibody titers against a bovine betacoronavirus. The in-contact animals did not become infected.
Influenza virus A/H1N1, which is currently causing a pandemic, contains gene segments with ancestors in the North American and Eurasian swine lineages. To get insights into replication dynamics, clinical symptoms transmission pigs, we infected animals intranasally influenza A/Regensburg/D6/09/H1N1. Virus excretion inoculated pigs was detected nasal swabs from 1 day post-infection (p.i.) onwards developed generally mild symptoms, including fever, sneezing, discharge diarrhoea. Contact became...
Abstract Rapid and sensitive diagnostic approaches are of the utmost importance for detection humans animals infected by specific influenza virus subtype(s). Cascade-like diagnostics starting with use pan-influenza assays subsequent subtyping devices normally used. Here, we demonstrated a novel low density array combining 32 TaqMan ® real-time RT-PCR systems in parallel haemagglutinin (HA) neuraminidase (NA) subtypes avian porcine hosts. The sensitivity newly developed system was compared...
Domestic cats (Felis catus) are popular companion animals that live in close contact with their human owners. Therefore, the risk of a trans-species spreading event between domestic and humans is ever-present. Shortly after emergence severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) its rapid spread around world, role transmission cycle was questioned. In present study, first large-scale survey antibody occurrence cat population Germany conducted, order to assess incidence...
Cowpox virus (CPXV) was considered as uniform species within the genus Orthopoxvirus (OPV). Previous phylogenetic analysis indicated that CPXV is polyphyletic and isolates may cluster into different clades with two of these showing genetic similarities to either variola (VARV) or vaccinia viruses (VACV). Further analyses were initiated assess both diversity evolutionary background circulating CPXVs. Here we report full-length sequences 20 strains isolated from animal humans in Germany. A...
Abstract Emerging variants of concern (VOCs) are driving the COVID-19 pandemic 1,2 . Experimental assessments replication and transmission major VOCs progenitors needed to understand mechanisms 3 Here we show that spike protein (S) from Alpha (also known as B.1.1.7) Beta (B.1.351) had a greater affinity towards human angiotensin-converting enzyme 2 (ACE2) receptor than progenitor variant S(D614G) in vitro. Progenitor virus expressing (wt-S 614G ) showed similar kinetics nasal airway...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a pandemic with millions of infected humans and hundreds thousands fatalities. As the novel disease - referred to as COVID-19 unfolded, occasional anthropozoonotic infections animals by owners or caretakers were reported in dogs, felid species farmed mink. Further shown be susceptible under experimental conditions. The extent natural animals, however, is still largely unknown. Serological methods will useful tools for...
In May 2016, two cases of tick-borne encephalitis (TBE) were confirmed by serology (positive IgM and IgG antibodies against TBE virus (TBEV) in serum), with a possible link to raw milk cheese from goat farm region Baden-Württemberg, Germany not previously known as TBE-endemic. The outbreak investigation identified 32 consumers dairy products (29 consumers, one employee, owners) whom none had TBEV 3–8 weeks after consumption. Of the 27 notified State, reported consumption or suspected farm....
Abstract Variant of concern (VOC) Omicron-BA.1 has achieved global predominance in early 2022. Therefore, surveillance and comprehensive characterization advanced primary cell culture systems animal models are urgently needed. Here, we characterize recombinant spike gene mutants comparison with VOC Delta well-differentiated human nasal bronchial epithelial cells vitro, followed by vivo fitness hamsters, ferrets hACE2-expressing mice, immunized hACE2-mice. We demonstrate a spike-mediated...
The SARS-CoV-2 Omicron subvariants BA.1 and BA.2 exhibit reduced lung cell infection relative to previously circulating variants, which may account for their pathogenicity. However, it is unclear whether by BA.5, displaced these remains attenuated. Here, we show that the spike (S) protein of BA.5 exhibits increased cleavage at S1/S2 site drives cell-cell fusion entry with higher efficiency than its counterparts from BA.2. Increased depends on mutation H69Δ/V70Δ associated efficient...
Summary In March 2024, highly pathogenic avian influenza virus (HPAIV) clade 2.3.4.4b H5N1 infections in dairy cows were first reported from Texas, USA. Rapid dissemination to more than 190 farms 13 states followed. Here, we provide results of two independent experimental infection studies evaluating (i) oronasal susceptibility and transmission calves a US bovine isolate genotype B3.13 (H5N1 B3.13) (ii) lactating following direct mammary gland inoculation either or current EU wild bird euDG...
<title>Abstract</title> Mpox, caused by the monkeypox virus (MPXV; <italic>Orthopoxvirus monkeypox</italic>), is on rise in West and Central Africa. Most outbreaks are short-lived, but MPXV has recently larger epidemics driven sustained human-to-human transmission. It widely accepted that mpox originate zoonotic events. African rodents, especially squirrels, suspected to be involved emergence, no formal link human or nonhuman primate been established. Here, we describe an outbreak of a group...
Mortality in wild aquatic birds due to infection with highly pathogenic avian influenza viruses (HPAIV) is a rare event. During the recent outbreak of Germany, mortality H5N1 HPAIV was observed among mute and whooper swans as part rapid spread this virus. In contrast earlier reports, appeared be susceptible represented mainly affected species. We report gross histopathology distribution virus antigen that died after natural HPAIV. At necropsy, most reliable lesions were multifocal...
We investigated in a unique setup of animal models and human lung explant culture biological properties, including zoonotic potential, representative 2016 highly pathogenic avian influenza virus (HPAIV) H5N8, clade 2.3.4.4 group B (H5N8B), that spread rapidly huge ongoing outbreak series Europe caused high mortality waterfowl domestic birds. HPAIV H5N8B showed increased virulence with rapid onset severe disease Pekin ducks due to pronounced neuro- hepatotropism. Cross-species infection was...