- Plant Virus Research Studies
- Plant and Fungal Interactions Research
- Insect-Plant Interactions and Control
- Plant Disease Resistance and Genetics
- Plant Pathogenic Bacteria Studies
- Phytoplasmas and Hemiptera pathogens
- Plant Parasitism and Resistance
- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Cocoa and Sweet Potato Agronomy
- Plant-Microbe Interactions and Immunity
- Bacteriophages and microbial interactions
- Insect and Pesticide Research
- Vector-Borne Animal Diseases
- Insect symbiosis and bacterial influences
- Animal Virus Infections Studies
- Plant Disease Management Techniques
- Herpesvirus Infections and Treatments
- Phytochemical compounds biological activities
- Plant Pathogens and Fungal Diseases
- Chromosomal and Genetic Variations
- Horticultural and Viticultural Research
- Engineering and Information Technology
- Evolution and Genetic Dynamics
- Morinda citrifolia extract uses
Citrus Research Institute
2016-2025
Southwest University
2016-2025
Northeast Agricultural University
2025
Chinese Academy of Agricultural Sciences
2010-2025
Feed Research Institute
2025
Ministry of Agriculture and Rural Affairs
2025
Southwest Wisconsin Technical College
2023
Huazhong Agricultural University
2023
National Agricultural Library
2019-2020
Serco (United Kingdom)
2020
Significance RNAi-mediated antiviral immunity directs specific virus resistance by virus-derived siRNAs in contrast to broad-spectrum triggered innate host pattern recognition receptors. Here we show that induction of RNAi Arabidopsis is associated with production a genetically distinct class virus-activated (vasiRNAs) RNA-dependent RNA polymerase-1 target hundreds genes for silencing Argonaute-2. Production vasiRNAs induced viruses from two different supergroups families, targeted...
A common challenge in pathogen discovery by deep sequencing approaches is to recognize viral or subviral pathogens samples of diseased tissue that share no significant homology with a known pathogen. Here we report homology-independent approach for discovering viroids, distinct class free circular RNA encode protein and are infect plants only. Our involves analyzing the sequences total small RNAs infected obtained unique computational algorithm, progressive filtering overlapping (PFOR)....
Abstract Viruses with split genomes are classified as being either segmented or multipartite based on whether their genomic segments occur within a single virion across different virions. Despite variations in number and sequence during evolution, the of many viruses conserved untranslated regions (UTRs). In this study, we present methodology that combines RNA sequencing iterative BLASTn UTRs (https://github.com/qq371260/Iterative-blast-v.1.0) to identify new viral segments. Some novel...
Two novel negative-sense, single-stranded (ss) RNA viruses were identified in watermelon plants and named crinkle leaf-associated virus 1 2 (WCLaV-1 -2), respectively. The multipartite genomes consist of three molecules approximately 6.8, 1.4 1.3 kb. the deduced proteins RNA1 RNA3 show features resembling those members genus Phlebovirus Tenuivirus; however, predicted encoded by RNA2 are related to movement protein (MP) Ophiovirus Emaravirus. Furthermore, these two define a clade family...
An emerging virus-like flower yellowing disease (FYD) of green Sichuan pepper (Zanthoxylum armatum v. novemfolius) has been recently reported. Four new RNA viruses were discovered in the FYD-affected plant by virome analysis using high-throughput sequencing transcriptome and small RNAs. The complete genomes determined, based on sequence phylogenetic analysis, they are considered to be members genera Nepovirus (Secoviridae), Idaeovirus (unassigned), Enamovirus (Luteoviridae),...
In 2009, a new citrus viral disease caused by Citrus yellow vein clearing virus (CYVCV) was discovered in China. To more effectively monitor the presence of CYVCV, survey conducted 166 orchards from 11 major citrus-growing provinces China May 2014 to April 2016. all, 458 total 2,350 samples tested positive for demonstrating that is widely distributed this study, complete genome sequences 19 CYVCV isolates different and hosts were sequenced characterized. Comparisons whole-genome these as...
A new wheat viral disease exhibiting unusual symptoms - yellow stripes on leaves and slight dwarfism -was found in China. The bullet-shaped particles within the nucleus of infected leave cells, which possessed 180-210 nm length 35-40 width, were observed under transmission electron microscopy. putative wheat-infecting rhabdovirus vectored by leafhopper Psammotettix alienus was tentatively named striate virus (WYSV). full-length nucleotide sequence WYSV determined using transcriptome...
Currently, many viruses are classified based on their genome organization and nucleotide/amino acid sequence identities of capsid replication-associated proteins. Although biological traits such as vector specificities host range also considered, this later information is scarce for the majority recently identified viruses, characterized only from genomic sequences. Accordingly, sequences derived being frequently used major, if not only, criteria virus classification calls a full review...
Paris yunnanensis, also named as Rhizoma Paridis in the Chinese Pharmacopeia, is a perennial medicinal herb commonly grown Southwest China. However, several viruses have been found infecting this plant recent years. Using high-throughput sequencing (HTS) and Sanger sequencing, study obtained complete genome sequences of three capillovirus isolates one potyvirus isolate. Genomic phylogenetic analyses revealed that these are same virus newly reported capillovirus, polyphylla chlorotic mottle...
Staphylococcus aureus, a prevalent zoonotic pathogen, poses significant threat to skin wound infections. This study evaluates the bactericidal efficacy of self-assembled peptide hydrogels, PPI45 and PPI47, derived from defensin-derived PPI42, against S. aureus ATCC43300. The high-level preparation PPI47 was achieved with yields 1.82 g/L 2.13 g/L, which are 2.19 2.60 times yield PPI42. Additionally, critical micelle concentrations (CMCs) peptides at pH 7.4 for PPI45, were determined be 245...
The emergence and rapid development of antibiotic resistance poses a serious threat to global public health. Streptococcus suis (S. suis) is an important zoonotic pathogen, the its has made infections difficult treat. combination non-antibiotic compounds with antibiotics considered promising strategy against multidrug-resistant bacteria. However, mechanism by which metabolites act as adjuvant remains unclear. Here, we found that guanosine metabolism was repressed in S. suis. Exogenous...
Citrus yellow vein clearing virus (CYVCV), the causal agent of citrus disease, has been posing an emerging threat to production in China. In this study, we confirm that CYVCV is spring shoot leaf curl disease 'Shatangju' mandarin (Citrus reticulata Blanco), a major commercial cultivar Guangxi Province, Field surveys revealed high incidence typical viral symptoms, including curling and clearing, with detection rate 95.65% symptomatic samples. To verify association between field inoculated...
Alfalfa mosaic virus (AMV), a globally distributed plant pathogen in the genus Alfamovirus (family Bromoviridae), was first identified as causative agent of alfalfa disease 1931 (Hull R., 1969; Moreira AG et al., 2010). This has affected over 600 species across 70 families under natural and experimental conditions (Wang X 2023), is transmitted by aphids, seeds pollen, mechanical inoculation 1969). Typical infection symptoms include mild mottling, chlorotic local lesions, patterns, mottled...
Citrus tristeza virus (CTV) is one of the most important causal agents citrus diseases and exists as numerous strains. CTV replicated in phloem cells plants within family Rutaceae transmitted by a few aphid species. epidemics have caused death millions trees many regions all over world, where sour orange (Citrus aurantium) was used rootstock. Also production grapefruit (C paradisi) sweet sinensis) has been affected gives uplift to three prominent syndromes, namely quick-decline (tristeza),...
To identify the pathogens responsible for leaf yellowing symptoms on wheat samples collected from Jinan, China, we tested presence of three known barley/wheat yellow dwarf viruses (BYDV-GAV, -PAV, WYDV-GPV) (most likely pathogens) using RT-PCR. A sample that negative was selected small RNA sequencing. Twenty-five million sequences were generated, among which 5% viral origin. novel polerovirus discovered and temporarily named yellowing-associated virus (WLYaV). The full genome WLYaV...
A previously unreported disease affecting jujube (Ziziphus jujuba Mill) trees was observed in China (Liaoning province) 2015 and named yellow mottle (JYMD), due to prevalent symptoms on the leaves. Diseased plants produced also malformed discolored fruits. In an attempt identify possible causal agent of JYMD, high-throughput sequencing small RNA libraries performed a novel virus, tentatively mottle-associated emaravirus (JYMaV), identified characterized. Six genomic segments JYMaV were...
Many species of the genus Camellia are native to China, and several such as C. japonica have been cultivated garden plants for over thousand years. Virus-like symptoms recorded In this study, with various leaf were observed in Jiangxi Chongqing provinces. The composition potential viruses symptomatic was analyzed by next-generation sequencing six libraries prepared from total RNAs specimens 10 trees. Five new discovered sequenced tentatively named chlorotic ringspot (CaCRSVs), yellow virus...
Our knowledge of citrus viruses is largely skewed toward virus pathology in cultivated orchards. Little known about the diversity wild species. Here, we used a metatranscriptomics approach to characterize habitat within proposed center origin plants. We discovered total 44 isolates that could be classified into species Citrus tristeza and putative associated ampelovirus 1, 2, B family Closteroviridae , providing important information explore factors facilitating outbreaks evolutionary...