A5003202615- Plant Virus Research Studies
- Plant and Fungal Interactions Research
- Plant-Microbe Interactions and Immunity
- Insect-Plant Interactions and Control
- CRISPR and Genetic Engineering
- Transgenic Plants and Applications
- Plant Molecular Biology Research
- Plant Parasitism and Resistance
- Insect symbiosis and bacterial influences
- Plant Pathogenic Bacteria Studies
- Animal Virus Infections Studies
- Distributed and Parallel Computing Systems
- Insect Resistance and Genetics
- Bacteriophages and microbial interactions
- Phytoplasmas and Hemiptera pathogens
- Autophagy in Disease and Therapy
- Plant tissue culture and regeneration
- Drilling and Well Engineering
- Chromosomal and Genetic Variations
- Mosquito-borne diseases and control
- Plant Disease Resistance and Genetics
- RNA Research and Splicing
- Hydraulic Fracturing and Reservoir Analysis
- Peer-to-Peer Network Technologies
- Plant Reproductive Biology
China Agricultural University
2016-2025
Southwest Petroleum University
2023-2024
PetroChina Southwest Oil and Gas Field Company (China)
2023
University of California, Riverside
2009-2017
Plant (United States)
2009-2017
University of Minnesota
2009-2015
Dalian University of Technology
2012-2013
National University of Singapore
2004-2007
Anhui Institute of Optics and Fine Mechanics
2005-2006
Massachusetts Institute of Technology
2004
In diverse eukaryotic organisms, Dicer-processed, virus-derived small interfering RNAs direct antiviral immunity by RNA silencing or interference. Here we show that in addition to core dicing and slicing components of RNAi, the RNAi-mediated viral Arabidopsis thaliana requires host RNA-directed polymerase (RDR) 1 RDR6 produce secondary siRNAs following replication-triggered biogenesis primary siRNAs. We found two RDRs exhibited specificity targeting tripartite positive-strand genome cucumber...
Abstract Arabidopsis thaliana defense against distinct positive-strand RNA viruses requires production of virus-derived secondary small interfering RNAs (siRNAs) by multiple RNA-dependent polymerases. However, little is known about the biogenesis pathway and effector mechanism viral siRNAs. Here, we describe a mutant Cucumber mosaic virus (CMV-Δ2b) that silenced predominantly RNA-DEPENDENT POLYMERASE6 (RDR6)-dependent siRNA pathway. We show siRNAs targeting CMV-Δ2b SUPPRESSOR OF GENE...
Significance RNAi-mediated antiviral immunity directs specific virus resistance by virus-derived siRNAs in contrast to broad-spectrum triggered innate host pattern recognition receptors. Here we show that induction of RNAi Arabidopsis is associated with production a genetically distinct class virus-activated (vasiRNAs) RNA-dependent RNA polymerase-1 target hundreds genes for silencing Argonaute-2. Production vasiRNAs induced viruses from two different supergroups families, targeted...
Autophagy is a conserved defense strategy against viral infection. However, little known about the counterdefense strategies of plant viruses involving interference with autophagy. Here, we show that γb protein from Barley stripe mosaic virus (BSMV), positive single-stranded RNA virus, directly interacts AUTOPHAGY PROTEIN7 (ATG7). BSMV infection suppresses autophagy, and overexpression sufficient to inhibit Furthermore, silencing autophagy-related gene ATG5 ATG7 in Nicotiana benthamiana...
Plant viruses have been used as rapid and cost-effective expression vectors for heterologous protein in genomic studies. However, delivering large or multiple foreign proteins monocots insect pests is challenging. Here, we recovered a recombinant plant cytorhabdovirus, Barley yellow striate mosaic virus (BYSMV), use versatile platform cereals the small brown planthopper (SBPH, Laodelphax striatellus) vector. We engineered BYSMV to provide platforms simultaneous of three barley plants SBPHs....
Abstract Selective autophagy is a double-edged sword in antiviral immunity and regulated by various receptors. However, it remains unclear how to balance the opposite roles one receptor. We previously identified virus-induced small peptide called VISP1 as selective receptor that facilitates virus infections targeting components of RNA silencing. we show here can also inhibit mediating autophagic degradation viral suppressors silencing (VSRs). targets cucumber mosaic (CMV) 2b protein for...
Shoot apical meristems (SAM) are resistant to most plant viruses due RNA silencing, which is restrained by viral suppressors of silencing (VSRs) facilitate transient invasion the SAM. In many cases chronic symptoms and long-term virus recovery occur, but underlying mechanisms poorly understood. Here, we found that wild-type Cucumber mosaic (CMVWT) invaded SAM transiently, was subsequently eliminated from meristems. Unexpectedly, a CMV mutant, designated CMVRA harbors an alanine substitution...
RNA viruses encode various binding proteins that function in many steps of viral infection cycles. These as helicases, methyltransferases, RNA-dependent polymerases, silencing suppressors, chaperones, movement proteins, and so on. Although the bind genome during different stages infection, our knowledge about coordination their functions is limited. In this study, we describe a novel role for Barley stripe mosaic virus (BSMV) γb an enhancer αa helicase activity, show protein recruited by...
Liquid-liquid phase separation (LLPS) plays important roles in forming cellular membraneless organelles. However, how host factors regulate LLPS of viral proteins during negative-sense RNA (NSR) virus infection is largely unknown. Here, we used barley yellow striate mosaic (BYSMV) as a model to demonstrate regulation casein kinase 1 (CK1) and NSR viruses. We first found that the BYSMV phosphoprotein (P) formed spherical granules with liquid properties recruited nucleotide (N) polymerase (L)...
Abstract Potassium (K+) plays crucial roles in both plant development and immunity. However, the function of K+ plant–virus interactions remains largely unknown. Here, we utilized Barley yellow striate mosaic virus (BYSMV), an insect-transmitted cytorhabdovirus, to investigate interplay between viral infection homeostasis. The BYSMV accessory P9 protein exhibits viroporin activity by enhancing membrane permeability Escherichia coli. Additionally, increases uptake yeast (Saccharomyces...
Significance Forward genetics as an unbiased approach for the genetic dissection of cellular pathways has not been widely used to identify host genes essential antiviral RNAi. Here we report identification a phospholipid flippase component RNAi from forward screen developed in Arabidopsis thaliana . We also describe versatile computational pipeline capable identifying different types causal mutation, including deletion, insertion, and single-nucleotide whole-genome sequencing. show that...
ABSTRACT All well-characterized positive-strand RNA viruses[(+)RNA viruses] induce the formation of host membrane-bound viral replication complexes (VRCs), yet underlying mechanism and machinery for VRC remain elusive. We report here biogenesis topology Beet black scorch virus (BBSV) complex. Distinct cytopathological changes typical endoplasmic reticulum (ER) aggregation vesiculation were observed in BBSV-infected Nicotiana benthamiana cells. Immunogold labeling auxiliary protein p23...
Abstract As obligate intracellular phytopathogens, plant viruses must take advantage of hosts plasmodesmata and phloem vasculature for their local long-distance transports to establish systemic infection in plants. In contrast well-studied virus transports, molecular mechanisms related host genes governing trafficking are far from being understood. Here, we performed a forward genetic screening identify Arabidopsis thaliana mutants with enhanced susceptibility 2b-deleted mutant cucumber...
Summary P0 protein of some polerovirus members can target ARGONAUTE 1 ( AGO 1) to suppress RNA silencing. Although harbors an F‐box‐like motif reported be essential for interaction with S phase kinase‐associated SKP and silencing suppression, it is the autophagy pathway that was shown contribute degradation. Therefore, role P0– in suppression remains unclear. We conducted global mutagenesis comparative functional analysis encoded by Brassica yellows virus (BrYV) (P0 Br ). found several...
The barley stripe mosaic virus (BSMV) triple gene block 1 (TGB1) protein is required for cell-to-cell movement. However, little information available about how these activities are regulated by post-translational modifications. In this study, we showed that the BSMV Xinjiang strain TGB1 (XJTGB1) phosphorylated in vivo and vitro kinase CK2 from Nicotiana benthamiana. Liquid chromatography tandem mass spectrometry analysis phosphorylation assays demonstrated Thr-401 major site of XJTGB1...