Zebra Chip (ZC) is an emerging plant disease that causes aboveground decline of potato shoots and generally results in unusable tubers. This has led to multi-million dollar losses for growers the central western United States over past decade impacts livelihood farmers Mexico New Zealand. ZC associated with 'Candidatus Liberibacter solanacearum', a fastidious alpha-proteobacterium transmitted by phloem-feeding psyllid vector, Bactericera cockerelli Sulc. Research on this been hampered lack...

The accurate identification of citrus fruits is important for fruit yield estimation in complex orchards. In this study, the YOLOv7-tiny-BVP network constructed based on YOLOv7-tiny network, with as research object. This introduces a BiFormer bilevel routing attention mechanism, which replaces regular convolution GSConv, adds VoVGSCSP module to neck and simplified efficient layer aggregation (ELAN) partial (PConv) backbone network. improved model significantly reduces number parameters...

Abstract The citrus pest Cacopsylla citrisuga (Yang & Li), a vector for Citrus Huanglongbing (HLB), exhibits distinct sensitivity to temperature variations. This study utilized an age-stage, 2-sex life table evaluate the development and reproduction of C. across 5 temperatures (17, 20, 25, 28, 31 °C). findings indicate that can complete its cycle within range 17–28 °C, with optimal at 20 where highest survival fecundity rates in females were observed. An increase correlates decrease...

Abstract The 40S ribosomal protein SA (RPSA) functions as an important regulatory factor in plant resistance to abiotic stresses. However, the role of RPSA response virus infection is poorly understood. Citrus yellow vein clearing (CYVCV) has a significantly negative impact on citrus production, and its coat (CP) involved viral pathogenicity. In this study, we revealed interaction CP with Eureka lemon (ClRPSA-2) nucleus, membrane, endoplasmic reticulum Nicotiana benthamiana . Further...

Abstract BACKGROUND Citrus canker, a bacterial disease caused by Xanthomonas citri subsp. (Xcc), is one of the major threats to citrus industry. Inorganic copper (Cu) formulations such as Bordeaux mixture and Kocide 3000 are currently used control canker; however, they poorly water‐soluble have negligible plant transport, making systemic treatment canker difficult. RESULTS This study synthesized Cu 2 O@His one‐pot self‐assembly 2+ histidine for controlling canker. The results showed that had...

Citrus Huanglongbing (HLB), a highly devastating citrus disease, is associated with 'Candidatus Liberibacter asiacitus' (CLas), member of phloem-inhabiting α-proteobacteria. HLB can affect all cultivated and no cure currently available. Previous studies showed that Kaffir lime (Citrus hystrix), primarily grown in South Asia Southeast Asia, was tolerant to but the molecular mechanism remains unknown. In this study, gene expression profiling experiments were performed on HLB-tolerant C....

ABSTRACT We report here the complete genome sequence of “ Candidatus Liberibacter asiaticus” (strain Guangxi-1). The 1,268,237-bp with a 36.5% G+C content comprises 1,141 open reading frames, 44 tRNAs, and 3 rRNAs in circular chromosome.

ABSTRACT We report here the complete genome sequence of “ Candidatus Liberibacter africanus” strain PTSAPSY. The 1,192,232-bp with 34.5% G+C content comprises 1,017 open reading frames, 44 tRNAs, and three rRNAs in a circular chromosome.

Citrus Huanglongbing (HLB, yellow shoot disease) is one of the most serious citrus diseases worldwide. To better improve detection sensitivity, a droplet digital PCR (ddPCR) assay was developed for rapid 'Candidatus Liberibacter asiaticus' (Las), putative causal agent HLB. The sensitivity comparison using positive plasmid indicated that ddPCR superior to quantitative (qPCR) detecting and quantifying Las at low concentrations. 40 field samples also showed six 13 asymptomatic (46.15%) with...

Abstract In the year 2010, in a survey G uangxi P rovince, C hina, to detect and characterize phytoplasmas huanglongbing ( HLB )‐infected grapefruit itrus paradisi ) orchard, 87 leaf samples with symptoms of blotchy mottle were collected from symptomatic trees, 320 symptomless trees adjacent trees. Nested polymerase chain reaction PCR using universal phytoplasma primer set P1/P7 followed by f U 5/r 3 identified 7 (8.0%) positive but none samples. Of samples, 77 (88.5%) for ‘ andidatus...

Abstract Diaphorina citri is a global citrus pest. As vector insect, it can transmit the causative agents of huanglongbing, causing irreversible losses to industry. The acquisition genomic information provide molecular genetic basis for effective control D. . Here, DNBSEQ ™ , Oxford Nanopore Technologies, and Hi‐C technologies are applied generate high‐quality chromosome‐level genome size was 523.78 Mb with scaffold N50 47.05 distributed on 13 chromosomes. A total 250.64 (47.85%) repeat...

Asian citrus psyllid (Diaphorina citri, D. citri) is the important vector of "Candidatus Liberibacter asiaticus" (CLas), associated with Huanglongbing, most devastating disease worldwide. CLas can affect endosymbiont abundance citri. Here, we generated high-quality gut metagenomes Diaphorina citri on condition infected and uninfected. The dataset comprised 6616.74 M 6586.04 raw reads, overage, from uninfected strains, respectively. Taxonomic analysis revealed that a total 1046 species were...

We report here the draft genome sequence of "Candidatus Liberibacter americanus" strain PW_SP. The 1,176,071-bp genome, with 31.6% G+C content, comprises 948 open reading frames, 38 tRNAs, and three complete rRNAs.

The Asian citrus psyllid (ACP), Diaphorina citri Kuwayama, is the principal vector of Candidatus Liberibacter asiaticus (CLas) bacterium that causes Huanglongbing (HLB) disease. D. salivary glands (SG) an important barrier to transmission CLas. Despite its importance, transcriptome and proteome SG defense against CLas are unstudied in citri. In present study, we generated a comparative dataset infected uninfected using Illumina RNA-Seq technology. We obtained 407 differentially expressed...

Introduction Citrus chlorotic dwarf-associated virus (CCDaV) is an economically important citrus associated with leaf curling, deformation, and chlorosis found in China. Plants have evolved RNA silencing to defend against viral infections; however, the mechanism by which CCDaV suppresses remains unknown. Methods Six proteins encoded were ectopically expressed Nicotiana benthamiana 16c using pCHF3 vector identify RNA-silencing suppression activities. Results V2 protein suppressed local...

The Diaphorina citri Kuwayama (D. citri) is one of the major pests in citrus industry, which spreads Citrus Huanglongbing disease. It has developed resistance to chemical insecticides. Therefore, searching for greener solutions pest management critically important. main aim this study was evaluate repellent and insecticidal efficacy essential oils (EOs) from four species Myrtaceae plants: Psidium guajava (PG), Eucalyptus robusta (ER), tereticornis (ET), Baeckea frutescens (BF) against D....

Insecticide susceptibility is mainly determined by the insect host, but symbiotic bacteria are also an important affecting factor. In this study, we investigate relationship between structure of gut bacterial symbionts and insecticide in

Abstract Background Citrus fruits are economically and nutritionally important but have been severely affected by Huanglongbing disease (HLB), its natural spread is mainly the Asian Psyllid , Diaphorina citri Kuwayama (Hemiptera: Liviidae). Chemicals often used to control this pest, not sustainable. Meanwhile, there few environmentally friendly bioinsecticides D. in China. Results In study, an entomofungal pathogen wz-1 was isolated from a carcass field, which resulted cumulative mortality...

Huanglongbing (HLB) is one of the most destructive diseases in citrus production worldwide. Early detection HLB pathogens can facilitate timely removal infected trees field. However, low titer and uneven distribution host plants make reliable challenging. Therefore, development effective methods with high sensitivity imperative. This study reports a novel method, tandem repeat-based polymerase chain displacement reaction (TR-PCDR), for 'Candidatus Liberibacter asiaticus', widely distributed...

Siraitia grosvenorii, known as "Luohanguo or monk fruit", is a perennial vine belonging to the family Cucurbitaceae. It cultivated for its fruits, which are used Chinese traditional medicine treat throat, lung and intestine ailments, raw material extract sweet cucurbitane-glycosides sugar substitute sweeteners (Chen et al., 2007). The production of S. grosvenorii limited by viral diseases especially cucumber green mottle mosaic virus (CGMMV), papaya ringspot (PRSV), watermelon virus,...

Kuwayama (

Citrus chlorotic dwarf disease (CCDD) seriously affects the citrus industry. dwarf-associated virus (CCDaV) is speculated to be causal agent of CCDD. However, this speculation has not been confirmed by fulfilling Koch’s postulates. In study, an infectious clone comprising a 1.6-fold tandem CCDaV genome in binary vector pBinPLUS was constructed and agro-inoculated into ‘Eureka’ lemon (Citrus limon) seedlings through vacuum infiltration. At 60 days postinoculation, 25% developed symptoms...