A5046612407- Plant Virus Research Studies
- Phytoplasmas and Hemiptera pathogens
- Plant Pathogenic Bacteria Studies
- Cocoa and Sweet Potato Agronomy
- Plant and Fungal Interactions Research
- Plant Disease Management Techniques
- Insect-Plant Interactions and Control
- Plant Pathogens and Fungal Diseases
- Berry genetics and cultivation research
- Insect symbiosis and bacterial influences
- Plant Parasitism and Resistance
- Autoimmune and Inflammatory Disorders Research
- Inflammasome and immune disorders
- Insect and Pesticide Research
- Plant pathogens and resistance mechanisms
- Plant tissue culture and regeneration
- Immunodeficiency and Autoimmune Disorders
- Child and Adolescent Health
- Agriculture, Plant Science, Crop Management
- Adolescent and Pediatric Healthcare
- Plant Disease Resistance and Genetics
- Legume Nitrogen Fixing Symbiosis
- Transgenic Plants and Applications
- Plant Pathogens and Resistance
- Banana Cultivation and Research
Institute of Plant Molecular Biology
2015-2024
Czech Academy of Sciences
2009-2024
Czech Academy of Sciences, Biology Centre
2015-2024
University Hospital Brno
2004-2019
Masaryk University
2010-2017
Charles University
2016
University of Veterinary Sciences Brno
2003-2004
St. Anna Hospital
2004
Czech Academy of Sciences, Institute of Scientific Instruments
2004
Institute of Parasitology
2000
In Europe, two fastidious phloem-limited pathogens, ’ Candidatus Phytoplasma solani’ (16SrXII-A) and Arsenophonus phytopathogenicus’, are associated with rubbery taproot disease (RTD) syndrome basses richesses (SBR) of sugar beet, respectively. Both diseases can significantly reduce yield, especially when accompanied by root rot fungi. This study investigates the presence, geographic distribution genetic traits pathogens accompanying fungus, Macrophomina phaseolina , found on beet across...
There is a lack of published evidence on the importance methotrexate (MTX) dose and route administration both its efficacy adverse events in children with Juvenile Idiopathic Arthritis (JIA). We aimed to document our clinical practice based treat-to-target approach order support concept that better therapeutic effect achieved an optimal parenteral MTX associated clinically acceptable effects comparable those reported for oral treatment. Study inclusion criteria were indication new therapy...
Virus diseases of strawberry present several complex problems. More than 25 viruses have been described in the genus Fragaria thus far. Here, we describe a novel rhabdovirus, tentatively named virus 1 (StrV-1), that infects F. ananassa and vesca plants. Genomic sequences three distinct StrV-1 genotypes co-infecting single host were obtained using combined Illumina Ion Proton high-throughput sequencing. was transmitted to herbaceous plants via Aphis fabae A. ruborum, further mechanically...
A novel RNA virus infecting strawberry plants was discovered using high-throughput sequencing. The analyzed plant simultaneously infected with three different genetic variants of the virus, provisionally named (StrVA). Although StrVA is phylogenetically clustered several recently discovered, unclassified viruses, it has a smaller genome and unique features in its genomic organization. specific sensitive qPCR system for detection identified designed. survey conducted Czech Republic revealed...
This collaborative work by over 180 researchers from 40+ countries addresses the challenges posed “phantom agents”—putative pathogenic agents named in literature without supporting data on their existence. Those remain regulatory lists, creating barriers trade and plant certification. Historically identified based solely symptoms, these lack isolates or sequence data, making reliable detection risk assessment impossible. After reviewing 120 such across 10 key genera, we recommend removal...
Winter oilseed rape grown in several areas South Bohemia showed symptoms of stunting, leaf reddening and extensive malformation floral parts. Phytoplasmas were consistently observed by using electron microscopy only phloem tissue symptomatic plants. DNA isolated from infected healthy control plants was used PCR experiments. Primer pairs R16F2/R2, P1/P7 rpF2/R2, amplifying, respectively, 16S rDNA, rDNA plus spacer region the beginning 23S ribosomal protein gene L22 specific for phytoplasmas,...
Phytoplasmas were detected in apricots (Prunus armeniaca), peaches persica), plums domestica), plum hybrid salicina x cerasifera), sweet cherries (Cerasus avium), sour vulgaris), almond amygdalus persica) and Prunus cerasifera. A predominant phytoplasma associated with diseases of apricots, was identified as European stone fruit (= apricot chlorotic leaf roll phytoplasma). Sweet affected mainly by aster yellows (AY) phytoplasma. Furthermore, the obtained results can also provide evidence...
During their lifetime, perennial woody plants are expected to face multiple infection events. Furthermore, genotypes of individual virus species may co-infect the same host. This eventually lead a situation where harbor complex communities viral species/strains. Using high-throughput sequencing, we describe co-infection sweet and sour cherry trees with diverse genomic variants two closely related viruses, namely prunus F (PrVF) (CVF). Both viruses most homologous members Fabavirus genus...
A new phytoplasma was identified in naturally infected blackberry plants exhibiting witches' broom symptoms Portugal. The 16S rRNA gene sequence revealed that it is related to 'Candidatus Phytoplasma rubi' (16SrV-E ribosomal subgroup) and RFLP analysis a unique profile following MseI endonuclease digestion of R16F2n/R2 amplicons distinguished from the strains belonging previously established 16SrV subgroups. silico restriction analyses confirmed strain different all other reported group...
Three pairs of generic primers designed for specific reverse transcription polymerase chain reaction amplification part the cytorhabdoviruses genomes were successfully tested with a number red clover samples. Two infected plants showing irregular vein clearing, tissue necrosis and stunted growth symptoms selected further studies. Subsequently, two novel plant rhabdoviruses, tentatively named Trifolium pratense virus A (TpVA) B (TpVB), detected in plants. Sequence analyses nucleotide...
Raspberry plants, valued for their fruits, are vulnerable to a range of viruses that adversely affect yield and quality. Utilizing high-throughput sequencing (HTS), we identified novel virus, tentatively named raspberry enamovirus 1 (RaEV1), in three distinct plants. This study provides comprehensive characterization RaEV1, focusing on its genomic structure, phylogeny, possible transmission routes. Analysis nearly complete genomes from 14 RaEV1 isolates highlighted regions variance,...
A novel negative-sense single-stranded RNA virus showing genetic similarity to viruses of the genus Rubodvirus has been found in raspberry plants Czech Republic and tentatively named rubodvirus 1 (RaRV1). Phylogenetic analysis confirmed its clustering within group, albeit distantly related other members. screening 679 plant 168 arthropod samples from Norway revealed RaRV1 10 shrubs, one batch Aphis idaei, individual Orius minutus. Furthermore, a distinct isolate this was found, sharing 95%...
Aim of study: Thrips in raspberry crops are receiving attention Scandinavian production because the increasing sighting their presence flowers. Specific information on thrips species occurring and damage potential is scarce, making management challenging. Therefore, this study aimed to identify flowers cultivated raspberry, Rubus idaeus L., South Norway. Area Sogndal, Luster Frogn, Southern Norway Material methods: Adult were sampled from eight commercial orchards counties Vestland Viken...
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