A5057366386- Plant Virus Research Studies
- Phytoplasmas and Hemiptera pathogens
- Plant Pathogenic Bacteria Studies
- Berry genetics and cultivation research
- Plant Disease Management Techniques
- Cocoa and Sweet Potato Agronomy
- Plant and Fungal Interactions Research
- Insect-Plant Interactions and Control
- Education, Psychology, and Social Research
- Banana Cultivation and Research
- Corporate Governance and Law
- Plant Pathogens and Fungal Diseases
- Plant tissue culture and regeneration
- Plant-Microbe Interactions and Immunity
- Agriculture, Plant Science, Crop Management
- Transgenic Plants and Applications
- melanin and skin pigmentation
- Leadership, Courage, and Heroism Studies
- Mycorrhizal Fungi and Plant Interactions
- Resilience and Mental Health
- Plant Physiology and Cultivation Studies
- Optimism, Hope, and Well-being
- Biochemical Analysis and Sensing Techniques
- Botanical Research and Chemistry
- Corporate Governance and Management
Research and Breeding Institute of Pomology Holovousy
2017-2024
ORCID
2021
Central European Institute of Technology
2019
Central European Institute of Technology – Masaryk University
2019
A novel RNA virus infecting strawberry plants was discovered using high-throughput sequencing. The analyzed plant simultaneously infected with three different genetic variants of the virus, provisionally named (StrVA). Although StrVA is phylogenetically clustered several recently discovered, unclassified viruses, it has a smaller genome and unique features in its genomic organization. specific sensitive qPCR system for detection identified designed. survey conducted Czech Republic revealed...
A novel negative-sense single-stranded RNA virus showing genetic similarity to viruses of the genus Rubodvirus has been found in raspberry plants Czech Republic and tentatively named rubodvirus 1 (RaRV1). Phylogenetic analysis confirmed its clustering within group, albeit distantly related other members. screening 679 plant 168 arthropod samples from Norway revealed RaRV1 10 shrubs, one batch Aphis idaei, individual Orius minutus. Furthermore, a distinct isolate this was found, sharing 95%...
Phytoplasmas of the apple proliferation group cause diseases in various fruit trees, mainly apples, pears and apricots. Immunodominant membrane protein, or ‘Imp’, shows high inter‐ intraspecies gene variability, is thus frequently used for population studies these organisms. In this investigation, a survey was carried out ‘ Candidatus Phytoplasma pyri’ imp diversity Czech Republic. total, 12 genotypes that could be clustered into nine groups were identified. Using all publicly available...
Raspberry plants, valued for their fruits, are vulnerable to a range of viruses that adversely affect yield and quality. Utilizing high-throughput sequencing (HTS), we identified novel virus, tentatively named raspberry enamovirus 1 (RaEV1), in three distinct plants. This study provides comprehensive characterization RaEV1, focusing on its genomic structure, phylogeny, possible transmission routes. Analysis nearly complete genomes from 14 RaEV1 isolates highlighted regions variance,...
The mutations in gene for the melanocortin-4 receptor (MC4R) are most common etiology factors of monogenic obesity development. Recently, it has been shown that current life style a significant impact on phenotype MC4R mutation carriers - increases penetrance mutations. We aimed to study age time onset among carriers.DNA analysis was performed 268 unrelated Slovak and Moravian obese children adolescents 18 years 28 blood relatives >18 probands with mutation.Three different previously...
In total, 332 strawberry plants from 33 different locations in the Czech Republic with or without disease symptoms were screened by RT-PCR for presence of polerovirus 1 (SPV1) and five other viruses: mottle virus, crinkle mild yellow edge vein banding virus 1. SPV1 was detected 115 tested (35%), including 89 mixed infections. No correlation between viruses found. To identify potential invertebrate vectors, strawberry-associated species RT-PCR, found aphids Aphis forbesi, A. gossypii,...
Abstract High‐throughput sequencing has opened up possibilities of detecting new plant pathogens, especially viruses, with relative ease. However, information about biological characteristics and relevance to production is usually lacking. Recently, a strawberry cytorhabdovirus named virus 1 (StrV‐1) was described characterized at molecular level. The can be transmitted by aphids commonly found in commercial plantations. Here, we studied the impact sole StrV‐1 infection on two...
Background: Hexamerins are the most abundant proteins in haemolymph of larvae and pupae honeybee (Apis mellifera) red flour beetle (Tribolium castaneum).They serve as a source aminoacids for development non-feeding pupal stage.Furthermore, they act juvenile hormonebinding proteins, however, details this interaction remain unknown.Methods: We have isolated hexamerins from tribolium solved their structures by X-ray crystallography cryoelectron microscopy, respectively.Results: The structure...
‘Candidatus Phytoplasma spp.’ are pathogenic bacteria that infect many plant species. pyri’, one of the members 16SrX group causes pear decline disease adversely affects crops. To describe prevalence ‘Ca. P. pyri’ genotypes in Czech Republic, 143 samples were collected from 41 locations including commercial orchards as well trees along roads. was detected by PCR 115 samples, and it possible to determine imp gene genotype 84 samples. The most frequent A1, B1, C, which identified 71%...