A5025913432- Plant Virus Research Studies
- Plant and Fungal Interactions Research
- Phytoplasmas and Hemiptera pathogens
- Plant Pathogenic Bacteria Studies
- Cocoa and Sweet Potato Agronomy
- Plant Disease Resistance and Genetics
- Plant Disease Management Techniques
- Plant-Microbe Interactions and Immunity
- Plant Pathogens and Resistance
- Plant pathogens and resistance mechanisms
- Berry genetics and cultivation research
- Plant tissue culture and regeneration
- Insect-Plant Interactions and Control
- Transgenic Plants and Applications
- Plant Pathogens and Fungal Diseases
- Insect symbiosis and bacterial influences
- Agriculture, Plant Science, Crop Management
- Genomics, phytochemicals, and oxidative stress
- Animal Virus Infections Studies
- Banana Cultivation and Research
- Legume Nitrogen Fixing Symbiosis
- Agriculture and Biological Studies
- Agronomic Practices and Intercropping Systems
- Plant Stress Responses and Tolerance
- Garlic and Onion Studies
Institute of Plant Molecular Biology
2015-2024
Czech Academy of Sciences, Biology Centre
2015-2024
Czech Academy of Sciences
2012-2024
Institut für Bodenkultur und Pflanzenbau
2006
Quadram Institute
1993
Biotechnology and Biological Sciences Research Council
1993
Czech Academy of Sciences, Institute of Experimental Botany
1988
Research Institute of Agricultural Engineering
1987
Czech Agrifood Research Center
1987
Significance Our article presents the most comprehensive reconstruction of evolutionary and phylogeographic history a major plant pathogen brassica vegetables in Eurasia. Sampling across such large landmass poses considerable challenges, our study attempts to describe spatial temporal patterns migration for on scale. molecular clock analyses show that pathways turnip mosaic potyvirus retrace some historical trade arteries Silk Road. This demonstrates how genetic analysis can provide...
Members of the genus Luteovirus are responsible for economically destructive plant diseases worldwide. Over past few years, three luteoviruses infecting Prunus trees have been characterized. However, biological properties, prevalence, and genetic diversity those viruses not yet studied. High-throughput sequencing samples various wild, cultivated, ornamental species enabled identification four novel in which we obtained complete or nearly genomes. Additionally, identified another new putative...
This collaborative work by over 180 researchers from 40+ countries addresses the challenges posed “phantom agents”—putative pathogenic agents named in literature without supporting data on their existence. Those remain regulatory lists, creating barriers trade and plant certification. Historically identified based solely symptoms, these lack isolates or sequence data, making reliable detection risk assessment impossible. After reviewing 120 such across 10 key genera, we recommend removal...
Winter oilseed rape grown in several areas South Bohemia showed symptoms of stunting, leaf reddening and extensive malformation floral parts. Phytoplasmas were consistently observed by using electron microscopy only phloem tissue symptomatic plants. DNA isolated from infected healthy control plants was used PCR experiments. Primer pairs R16F2/R2, P1/P7 rpF2/R2, amplifying, respectively, 16S rDNA, rDNA plus spacer region the beginning 23S ribosomal protein gene L22 specific for phytoplasmas,...
During their lifetime, perennial woody plants are expected to face multiple infection events. Furthermore, genotypes of individual virus species may co-infect the same host. This eventually lead a situation where harbor complex communities viral species/strains. Using high-throughput sequencing, we describe co-infection sweet and sour cherry trees with diverse genomic variants two closely related viruses, namely prunus F (PrVF) (CVF). Both viruses most homologous members Fabavirus genus...
Five isolates of a new member the family Closteroviridae, tentatively named blackcurrant leafroll-associated virus 1 (BcLRaV-1), were identified in currant. The 17-kb-long genome codes for 10 putative proteins. replication-associated polyprotein has several functional domains, including papain-like proteases, methyltransferase, Zemlya, helicase, and RNA-dependent RNA polymerase. Additional open reading frames code small protein predicted to integrate into host cell wall, heat-shock 70...
Zinc is essential for the functioning of numerous proteins in plants. To investigate how Zn homeostasis interacts with virus infection, Zn-tolerant Noccaea ochroleucum plants exposed to deficient (Zn'0'), optimal (Zn10), and excess (Zn100) concentrations, as well Cd amendment, were infected Turnip yellow mosaic (TYMV). Imaging analysis fluorescence kinetics from μs (OJIP) minutes (Kautsky effect, quenching analysis) time domain revealed strong patchiness systemic virus-induced photosystem II...
Apple hammerhead viroid (AHVd) was detected in the apple cultivar ‘Šampion’ and mixed infection with Solanum nigrum ilarvirus 1 (SnIV-1) cultivars ‘Selena’ ‘Jonagored Supra’, using a high-throughput sequencing method. Experiments were conducted to eliminate both pathogens apples meristem tip cultures combination antivirotics ribavirin, rimantadine, zidovudine. Elimination of verified by repeated RT-PCR qRT-PCR assays after 7–11 months. SnIV-1 from all successful each three at concentrations...
Raspberry plants, valued for their fruits, are vulnerable to a range of viruses that adversely affect yield and quality. Utilizing high-throughput sequencing (HTS), we identified novel virus, tentatively named raspberry enamovirus 1 (RaEV1), in three distinct plants. This study provides comprehensive characterization RaEV1, focusing on its genomic structure, phylogeny, possible transmission routes. Analysis nearly complete genomes from 14 RaEV1 isolates highlighted regions variance,...
The Erysimum latent virus (ELV), a tymovirus, was first isolated from several wild and cultivated brassicas in Germany. Its virions were shown to be serologically distinct those of the turnip yellow mosaic (TYMV), which is also found plants European countries but other parts world. TYMV ELV among plant viruses have had their genomes sequenced, when tymovirus it that, phylogenies, probably basal outlier all tymoviruses. Here, we report near-complete genomic sequence another isolate Czechia. This 1990