A5111678031- Plant Virus Research Studies
- Plant and Fungal Interactions Research
- Plant Pathogenic Bacteria Studies
- Plant Disease Management Techniques
- Phytoplasmas and Hemiptera pathogens
- Plant Disease Resistance and Genetics
- Plant-Microbe Interactions and Immunity
- Insect-Plant Interactions and Control
- Plant Physiology and Cultivation Studies
- Plant Pathogens and Fungal Diseases
- Bacteriophages and microbial interactions
- Berry genetics and cultivation research
- Animal Virus Infections Studies
- Fungal Plant Pathogen Control
- Research in Cotton Cultivation
- Plant pathogens and resistance mechanisms
- Powdery Mildew Fungal Diseases
- Horticultural and Viticultural Research
- Plant and animal studies
- Banana Cultivation and Research
- Insect and Pesticide Research
- Transgenic Plants and Applications
- Insect Resistance and Genetics
- Cocoa and Sweet Potato Agronomy
- CRISPR and Genetic Engineering
Julius Kühn-Institut
2010-2024
Institut für Bodenkultur und Pflanzenbau
2002-2007
Universität Hamburg
2004
Federal Office for Food and Agriculture
1988-2003
Physikalisch-Technische Bundesanstalt
1988-2002
The complete nucleotide sequence of the RNA an aphid non-transmissible plum pox virus (PPV-NAT) isolate has been determined from five overlapping cDNA clones. prepared by primer extension was used to determine 5' terminus. assembled is 9741 nucleotides in length, excluding a 3' terminal poly(A) sequence. One large open reading frame starts at positions 36 38 and terminated with UAG codon 9522 9524. putative start located 147 149. encoded polyprotein predicted Mr 353.8K. Comparison cistrons...
The nucleotide sequence (9306 nucleotides) of cDNA clones apple stem pitting virus (ASPV) obtained from a double-stranded RNA template, extracted diseased plant tissue, was determined. genome is composed five open reading frames (ORFs) encoding putative proteins with M(r)s 247083, 25147, 12832, 7429 and 43712, has poly(A) tail. Using two oligonucleotides designed the ASPV information 1598 bp fragment near 3' terminus viral RNA, containing coat protein M(r) 43,766, amplified vein yellows...
The 5 ′ -terminal genomic region (8597 nt) of little cherry virus (LChV), a mealybug-borne closterovirus, was cloned from double-stranded RNA, and its sequence determined to complete the 16934 nt monopartite LChV RNA genome. In 5′ 3′ direction, encompasses ORF 1a, encoding conserved replicative domains methyltransferase helicase, 1b, polymerase. ORFs 1a 1b partially overlap (in 0/ 1 configuration), replicase is probably expressed by ribo- somal frameshifting as fusion product with molecular...
The nucleotide sequence (7383 nucleotides) of a newly identified member the genus Capillovirus, cherry virus A (CVA), was obtained from cDNA clones. generated dsRNA extracted plant tissue infected with little (LCV). Small amounts LCV served as template nucleic acid and enabled construction library which, unexpectedly, 7.5% recombinant plasmids were specific for CVA. genome organization CVA resembles that apple stem grooving (ASGV), type Capillovirus is composed 266 kDa polyprotein (ORF1), 52...
Abstract Isolates of apple stem pitting (ASP) and pear vein yellows (PVY) were sap transmitted from cultivars, respectively, to Nicotiana occidentalis ssp. obliqua. Total nucleic acid extraction, including a proteinase Kdigestion leaf homogenates prior phenol/chloroform was limited use for RNA template preparation. Amplification products obtained N. tissue. Three primer combinations near the viral 3′‐ terminus resulted in amplification 264 bp, 610bp 1548bp. The specificity confirmed by...
ABSTRACT Little cherry disease (LChD) is a complex and serious viral of cherry. Although originally described almost 70 years ago, there has been little progress in identifying the causal agent due to difficulty obtaining purified virus from infected trees. This problem was partially overcome 1997 when compete sequence closterovirus associated with LChD, (LChV), published. could be some, but not all, trees indicating that another also involved. We report here partial characterization second...
An isolate of Strawberry mottle virus (SMoV) was transferred from Fragaria vesca to Nicotiana occidentalis and Chenopodium quinoa by mechanical inoculation. Electron micrographs infected tissues showed the presence isometric particles approximately 28 nm in diameter. SMoV-associated tubular structures were also conspicuous, particularly plasmodesmata C. quinoa. DsRNA extraction SMoV-infected N. yielded two bands 6.3 7.8 kbp which cloned sequenced. Gaps sequence, including 5' 3' ends, filled...
The nucleotide sequence (5966 nucleotides) of cDNA clones strawberry mild yellow edge-associated potexvirus was determined. genome contains six open reading frames (ORFs) encoding putative proteins with M rs 149423, 25344, 11576, 8079, 25714 and 11216. In the first three coat protein considerable similarity found to comparable polypeptides potexviruses potato virus X, clover mosaic virus, narcissus papaya white lily X.
Fire blight, a plant disease of economic importance caused by Erwinia amylovora, may be controlled the application bacteriophages. Here, we provide complete genome sequences and annotation three E. amylovora-specific phages isolated in North America genomic information about bacteriophage induced mitomycin C treatment an tasmaniensis strain that is antagonistic for amylovora. The American resemble two already-described viral genomes, whereas phage displays singular sequence BLAST searches.
This collaborative work by over 180 researchers from 40+ countries addresses the challenges posed “phantom agents”—putative pathogenic agents named in literature without supporting data on their existence. Those remain regulatory lists, creating barriers trade and plant certification. Historically identified based solely symptoms, these lack isolates or sequence data, making reliable detection risk assessment impossible. After reviewing 120 such across 10 key genera, we recommend removal...
A physical map of cDNA clones prepared from dsRNA associated with the MY-18 source strawberry mild yellow edge (SMYE) was constructed and 854 nucleotides adjacent to 3' poly(A) tail were sequenced. The larger open reading frame product Mr 25714 showed considerable amino acid homology coat protein cistrons six potexviruses two carlaviruses. second 11216 encoded completely within cistron, but in a different frame, has similarities potexvirus polypeptides. ORF fused Protein gene an expression...
Strawberry mottle virus (SMoV) is probably the most important to infect strawberry (Fragaria spp.). All species of are susceptible SMoV, resulting in severe losses both fruit and runner yield. However, due absence definitive symptoms commercial varieties, only effective means detecting SMoV by transmission indicator plants. In this study, we describe a reverse transcription-polymerase chain reaction (RT-PCR) method for detection Fragaria spp. with use primers specific noncoding regions (NCR)...