A5064488182- Plant Physiology and Cultivation Studies
- Horticultural and Viticultural Research
- Plant tissue culture and regeneration
- Plant Reproductive Biology
- Plant Pathogens and Fungal Diseases
- Berry genetics and cultivation research
- Particle physics theoretical and experimental studies
- Education, Psychology, and Social Research
- High-Energy Particle Collisions Research
- Quantum Chromodynamics and Particle Interactions
- Plant Virus Research Studies
- Geological Formations and Processes Exploration
- Postharvest Quality and Shelf Life Management
- Plant Pathogenic Bacteria Studies
- Banana Cultivation and Research
- Fungal Plant Pathogen Control
- Flowering Plant Growth and Cultivation
- Plant Disease Management Techniques
- Particle Accelerators and Free-Electron Lasers
- Enterobacteriaceae and Cronobacter Research
- Nuclear physics research studies
- Microbial Community Ecology and Physiology
- Salmonella and Campylobacter epidemiology
- Plant and Fungal Interactions Research
- Particle accelerators and beam dynamics
Research and Breeding Institute of Pomology Holovousy
2015-2025
ORCID
2021-2022
Nuclear Research Institute Rez (Czechia)
2004-2021
Brno University of Technology
2014-2016
Institute of Oncology Ljubljana
1998
Institute of Physics
1979-1991
Joint Institute for Nuclear Research
1985-1990
Institute for High Energy Physics
1986
Czech Academy of Sciences, Institute of Physics
1975-1981
University of Wisconsin–Madison
1976-1977
The amount and structure of genetic diversity in dessert apple germplasm conserved at a European level is mostly unknown, since all studies conducted Europe until now have been performed on regional or national collections. Here, we applied common set 16 SSR markers to genotype more than 2,400 accessions across 14 collections representing three broad geographic regions (North + East, West South) with the aim analyze extent, distribution variation resources Europe. A Bayesian model-based...
Deciphering the genetic control of flowering and ripening periods in apple is essential for breeding cultivars adapted to their growing environments. We implemented a large Genome-Wide Association Study (GWAS) at European level using an association panel 1,168 different genotypes distributed over six locations phenotyped these phenological traits. The was genotyped high-density SNPs Axiom®Apple 480 K SNP array. ran GWAS with multi-locus mixed model (MLMM), which handles putatively...
Sweet cherry (Prunus avium L.) is an economically important and profitable stone fruit species grown in the temperate subtropical climates, bearing fruits with unique flavour nutritional benefits. There also a long tradition of sweet breeding Czech Republic. We report here evaluation vegetative characters 27 cultivars Research Breeding Institute Pomology (RBIP) genebank plantation. The were evaluated phenotypically using standard descriptors for cherries. Established ‘Burlat’ (early...
An investigation on the sugar compositions of lipopolysaccharides (O antigens) derived from 45 well–defined Citrobacter serotypes was carried out. As a result, chemical classification into 20 chemotypes achieved, which 11 were identical with occuring in Salmonella and Escherichia coli. All contained glucosamine, glucose, heptose, 2‐keto‐3‐deoxyoctonate galactose, latter, some cases, very small amounts. These sugars probably represent building blocks basal core polysaccharides, whose...
Sweet cherry (Prunus avium L.) is a temperate fruit species whose production might be highly impacted by climate change in the near future. Diversity of plant material could an option to mitigate these risks enabling producers have new cultivars well adapted environmental conditions. In this study, subsets sweet collections 19 European countries were genotyped using 14 SSR. The objectives study (i) assess genetic diversity parameters, (ii) estimate levels population structure, and (iii)...
In sweet cherry (Prunus avium L.), quantitative trait loci have been identified for fruit maturity, colour, firmness, and size to develop markers marker-assisted selection. However, resolution is usually too low in those analyses directly target candidate genes, some associations are missed. contrast, genome-wide association studies performed on broad collections of accessions, assemblies reference sequences from Tieton Satonishiki cultivars enable identification single nucleotide...
The vertex \ensuremath{\gamma}\ensuremath{\rightarrow}3\ensuremath{\pi} has been investigated in the reaction of pion pair production by pions nuclear Coulomb field, ${\ensuremath{\pi}}^{\ensuremath{-}}$+(Z,A)\ensuremath{\rightarrow}${\ensuremath{\pi}}^{\ensuremath{-}}$+${\ensuremath{\pi}}^{0}$+(Z,A), region low-invariant-mass ${\ensuremath{\pi}}^{\mathrm{\ensuremath{-}}}$${\ensuremath{\pi}}^{0}$ pairs. experiment carried out 40-GeV beam IHEP accelerator (Serpukhov) with SIGMA spectrometer....
The aim of this study was to compare traditional hierarchical clustering techniques and principal coordinate analysis (PCoA) with the model-based Bayesian cluster analyses in relation subpopulation differentiation based on breeding history geographical origin apple (Malus×domestica Borkh.) cultivars landraces. We presented use a set 10 microsatellite (SSR) loci for genetic diversity structure 273 accessions from national resources. These SSR yielded total 113 polymorphic alleles, 5-18...
SUMMARY The biochemical and serological characters of 36 unclassified cultures are described, according to these the organisms belong genera Citrobacter, Aerobacter Hafnia. While studying antigenic relationships O antigens known relationship between some serotypes Citrobacter Salmonella was confirmed; new with S. locarno, kentucky, uccle tranoroa described. Hafnia Shigella flexneri 4a two a provisional group were confirmed.
The profile of secondary metabolites occurring in apple cuticular layer is not only characteristic for particular cultivar, but also dynamically reflects various external factors growing environment. In this study, the possibility to authenticate samples based on analysis their extracts was investigated. Ultra-high-performance liquid chromatography coupled high resolution tandem mass spectrometry (UHPLC-HRMS/MS) employed obtaining metabolomic fingerprints. total, 274 authentic samples, four...
Genetic diversity and genetic relationships of Czech apple cultivars were evaluated. Trees 33 97 reference foreign analysed using the set 10 SSR (simple sequence repeat) primer pairs. The total 89 polymorphic alleles amplified, while number per locus ranged from 4 to 14. dendrogram, based on Jaccard's similarity coefficient, divided into three major groups: Cox's Orange Pippin, McIntosh Golden Delicious ancestries. clustering highly depended pedigree origin cultivars. Spontaneous mutated...
The effect of the chemotherapy with ribavirin on elimination pome fruit viruses from in vitro grown plants infected apple cv. Fragnance has been investigated. results ELISA and RT-PCR testing proved presence mixed infection Apple stem pitting virus (ASPV), chlorotic leaf spot (ACLSV) grooving (ASGV) initial field-grown tree this cultivar. Obtained actively growing shoots well-developed leaves shoot tips were subsequently used for ribavirin. Attempts to fully eliminate by lower concentration...
We have developed a rapid shoot multiplication procedure for in vitro propagation of blue honeysuckle (Lonicera kamtschatica [Sevast.] Pojark). Shoot tips two genotypes 20/1 and Altaj were successfully established micropropagated on the Murashige Skoog (MS) based media containing different concentrations 6-benzylaminopurine (BAP). Multiplication rates varied depending genotype concentration BAP. The highest rate was obtained that produced 10.5 ± 0.7 shoots (longer than 10 mm) MS medium 2...
Agriculture has always been an important part of human evolution. Traditionally, farming is changing and developing with regard to challenges it faces. The major modern agriculture are food nutrition safety for the growing world population. Promoting species genetic diversity in appears be approach dealing those challenges. Gene banks all around play a crucial role preserving plant resources future crop improvements. germplasm can preserved different ways, depending on or form stored tissue....
A novel negative-sense single-stranded RNA virus showing genetic similarity to viruses of the genus Rubodvirus has been found in raspberry plants Czech Republic and tentatively named rubodvirus 1 (RaRV1). Phylogenetic analysis confirmed its clustering within group, albeit distantly related other members. screening 679 plant 168 arthropod samples from Norway revealed RaRV1 10 shrubs, one batch Aphis idaei, individual Orius minutus. Furthermore, a distinct isolate this was found, sharing 95%...
A new detailed map of Bouguer gravity anomalies covers an area c. 13 500 km 2 the Lugian Unit in Bohemian Massif (Czech Republic, Germany and Poland).It is limited approximately by towns Bautzen (NW), Zlotoryja (NE), Červený Kostelec (SE) Roudnice nad Labem (SW).An extensive low about 120 long (W-E) 40 wide (N-S) most remarkable feature maps derived from it.This central (CLGL), adjacent to Saxothuringian West but it disappears high Kaczawa Complex Intra Sudetic Basin East.Five partial lows,...
The objective of this study was to investigate the possibility optimizing routine tissue culture methods proliferate two sweet cherry cultivars Karešova and Rivan. Shoot tips genotypes were successfully established in vitro. Six proliferation MS media containing 1, 2 4 mg/l BAP (6-benzylaminopurine), 0.5 1 TDZ (thidiazuron) or 10 2iP (6-(γ, γ-dimethylallylamino)purine) tested. highest rate (3.0 ± 0.1) obtained for Rivan on medium BAP. In case cultivar Karešova, any cytokinins tested did not...
A new cross-border gravity map on the scale of 1:200,000 covering 14,900 km 2 SE Saxony and NW Bohemia was compiled.It is limited by sites Grimma (NW), Karlovy Vary (SW), Neratovice (SE) Bautzen (NE).Three positive regions -(a) Lusatian Anticline, (b) part North Saxon Syncline (c) Teplá-Barrandian Unit were delimited.Separation Bouguer anomalies into regional residual components together with Linsser filtering provided three types derived maps (regional, density boundaries) for geological...