A5050838967- Pesticide Residue Analysis and Safety
- Metabolomics and Mass Spectrometry Studies
- Diet and metabolism studies
- Mass Spectrometry Techniques and Applications
- Alcohol Consumption and Health Effects
- Adipose Tissue and Metabolism
- Fatty Acid Research and Health
- Lipoproteins and Cardiovascular Health
- Horticultural and Viticultural Research
- Insect and Pesticide Research
- Peroxisome Proliferator-Activated Receptors
- Clinical Nutrition and Gastroenterology
- Cancer, Lipids, and Metabolism
- Identification and Quantification in Food
- Liver Disease and Transplantation
- Insect-Plant Interactions and Control
- Potato Plant Research
- Advanced Chemical Sensor Technologies
- Drug-Induced Hepatotoxicity and Protection
- Ferroptosis and cancer prognosis
- Nutrition and Health in Aging
- Plant Surface Properties and Treatments
- Carcinogens and Genotoxicity Assessment
- Aquatic Ecosystems and Biodiversity
- Silymarin and Mushroom Poisoning
University of Chemistry and Technology, Prague
2017-2025
Nonalcoholic fatty liver disease (NAFLD) is the most common chronic disease. n‐3 polyunsaturated acids (n‐3‐PUFAs) have been reported to ameliorate progression of NAFLD in experimental studies; however, clinical trials yielded contradictory results. The aim our study was assess effects n‐3‐PUFA administration on lipid metabolism and patients with metabolic syndrome. Sixty syndrome were randomized a double‐blind placebo‐controlled trial (3.6 g/day vs. placebo). During 1‐year follow‐up,...
Gut microbes have been recognized to convert human bile acids by deconjugation, dehydroxylation, dehydrogenation, and epimerization of the cholesterol core, but ability re-conjugate them with amino as an additional conversion has recently described. These new are known microbially conjugated (MCBAs). The aim this study was evaluate MCBAs diversity produced gut microbiota through a metabolomics approach. In study, fresh fecal samples from healthy donors were evaluated explore re-conjugation...
Meat adulteration is a significant economic problem as it can result in substantial gains and loss of consumers' trust the food industry. Addition bulking agent masking addition water into minced meat fraudulent practice that very difficult to detect. The quality be assessed by measurement total net protein, however methods used measure such property are not able cope with quite sophisticated modern-day practices. In our study, we potential recently introduced Rapid Evaporative Mass...
Abstract Statins, besides being powerful cholesterol-lowering drugs, also exert potent anti-proliferative activities. However, their anti-cancer efficacy differs among the individual statins. Thus, aim of this study was to identify biological pathways affected by statins in an vitro model human pancreatic cancer. The performed on a cancer cell line MiaPaCa-2, exposed all commercially available (12 μM, 24 h exposure). DNA microarray analysis used determine changes gene expression treated...
Abstract Introduction Regular physical activity and dietary variety are modifiable influential factors of health outcomes. However, the cumulative effects these behaviors not well understood. Metabolomics may have a promising research potential to extend our knowledge use it in attempts find long-term sustainable personalized approach exercise diet recommendations. Objective The main aim was investigate effect 12 week very low carbohydrate high fat (VLCHF) high-intensity interval training...
The profile of secondary metabolites occurring in apple cuticular layer is not only characteristic for particular cultivar, but also dynamically reflects various external factors growing environment. In this study, the possibility to authenticate samples based on analysis their extracts was investigated. Ultra-high-performance liquid chromatography coupled high resolution tandem mass spectrometry (UHPLC-HRMS/MS) employed obtaining metabolomic fingerprints. total, 274 authentic samples, four...
Abstract In the European Union, all chemical substances that are manufactured, imported or used in tonnages equal higher than one tonne per year must be registered under REACH regulation. This regulatory framework requires collection and dissemination of detailed information on properties, uses, potential health environmental impacts chemicals. However, some these chemicals may pose emerging risks food chain, necessitating development approaches to identify assess such risks. The SCREENER...
Alterations in lipid metabolism mediated by oxidative stress play a key role the process of atherosclerosis and superimposed thrombosis; these can lead to acute coronary syndrome (ACS) ischemic stroke (AIS). Multiple studies have shown that formation atheromatous lesions is initiated oxidation low-density lipoproteins incorporated into intima vessel wall. Here, we studied lipids plasma samples from three cohorts: 61 patients with ACS (group A), 49 AIS D), 82 controls K). Untargeted...
Inflammatory bowel disease, encompassing Crohn’s disease and ulcerative colitis, is a persistent immune-mediated inflammatory gastrointestinal disease. This study investigates the role of growth differentiation factor 15 in severe IBD cases, aiming to identify reliable parameter assess severity monitor activity. We analyzed plasma samples from 100 patients undergoing biologic therapy for 50 control subjects. Our analysis included evaluations GDF-15 levels, markers, clinical features....
Abstract Parenteral nutrition (PN) is often associated with the deterioration of liver functions (PNALD). Omega-3 polyunsaturated fatty acids (PUFA) were reported to alleviate PNALD but underlying mechanisms have not been fully unraveled yet. Using omics´ approach, we determined serum and lipidome, proteome, bile acid profile as well markers inflammation oxidative stress in rats administered either ω-6 PUFA based lipid emulsion (Intralipid) or ω-6/ω-3 blend (Intralipid/Omegaven) via enteral...
Omega-3 polyunsaturated fatty acids (ω-3PUFAs) are introduced into parenteral nutrition (PN) as hepatoprotective but may be susceptible to the lipid peroxidation while olive oil (OO) is declared more resistant. We aimed estimate how composition of PN mixture affects plasma and erythrocyte lipidome propensity oxidative stress. A cross-sectional comparative study was performed in a cohort adult patients who were long-term parenterally administered ω-3 PUFAs without (FO/–, n = 9) or with...
Lipids modified by oxidative stress are key players in atherosclerosis progression. Superimposed thrombosis with subsequent closure of the coronary artery leads to clinical manifestation acute syndrome (ACS). While several studies focusing on alterations lipid metabolism phase have been conducted, no information is available patients' lipidome over longer time periods. In current follow-up study, we analyzed plasma samples obtained from 17 patients three years after their ACS event (group...
Obstructive sleep apnea syndrome, characterized by repetitive episodes of tissue hypoxia, is associated with several metabolic impairments. Role fatty acids and lipids attracts attention in its pathogenesis for their effects. Parallelly, hypoxia-induced activation reverse tricarboxylic acid cycle (rTCA) reductive glutamine metabolism provides precursor molecules de novo lipogenesis. Gas-permeable cultureware was used to culture L6-myotubes chronic hypoxia (12%, 4% 1% O2) 13C labelled...
Abstract The global increase of toxin-producing cyanobacteria poses a serious risk to humans. Many investigations have shown that the cyanotoxin microcystin-LR induces hepatotoxicity in rodents. However, many these studies applied toxin intraperitoneally or used high oral concentrations, leading an unrealistically bioavailability toxin. Such approaches put into question how results translate human exposure scenarios. Epidemiology linked with and liver cancer humans, though by design cannot...
Milk thistle-based dietary supplements have become increasingly popular. The extract from milk thistle (Silybum marianum) is often used for the treatment of liver diseases because presence its active component, silymarin. However, co-occurrence toxic mycotoxins in these preparations quite frequent as well. objective this study was to investigate changes composition lipidome and other clinical characteristics experimental mice fed by a high-fat methionine-choline deficient diet inducing...
Recent studies have suggested a pathogenetic link between impaired mitochondria and Takotsubo syndrome (TTS), which is closely connected with catecholamine overstimulation, poor outcomes, changes in lipid metabolism. We investigated the metabolism at level of fatty acid β-oxidation intracellular lipidomic spectrum. The immortalized cell line HL-1 cardiomyocytes was used this study as an established vitro model TTS. cells were exposed to non-selective β-agonist isoprenaline (ISO) for acute (2...
The profile of secondary metabolites present in the apple cuticular layer is not only characteristic a particular cultivar; it also dynamically reflects various external factors growing environment. In this study, possibility authenticating samples by analyzing their extracts was investigated. Ultra-high-performance liquid chromatography coupled with high-resolution tandem mass spectrometry (UHPLC-HRMS/MS) employed for obtaining metabolomic fingerprints. A total 274 authentic from four...