A5071801441- Liver Disease Diagnosis and Treatment
- Liver Diseases and Immunity
- Pancreatic function and diabetes
- Liver Disease and Transplantation
- Skin and Cellular Biology Research
- Neonatal Health and Biochemistry
- Glycosylation and Glycoproteins Research
- Organ Transplantation Techniques and Outcomes
- Autoimmune Bullous Skin Diseases
- Organ and Tissue Transplantation Research
- Wound Healing and Treatments
- Liver physiology and pathology
- Gallbladder and Bile Duct Disorders
- Drug Transport and Resistance Mechanisms
- Pediatric Hepatobiliary Diseases and Treatments
- Proteoglycans and glycosaminoglycans research
- Cancer and Skin Lesions
- Reconstructive Surgery and Microvascular Techniques
- Cannabis and Cannabinoid Research
- Immunodeficiency and Autoimmune Disorders
- Polysaccharides and Plant Cell Walls
- Transplantation: Methods and Outcomes
- Metabolism and Genetic Disorders
- Heme Oxygenase-1 and Carbon Monoxide
- Drug-Induced Hepatotoxicity and Protection
Institute of Clinical and Experimental Medicine
2016-2025
Charles University
2016-2025
University Hospital Kralovske Vinohrady
2015-2025
King's College London
2020-2024
NHS Blood and Transplant
2018-2024
King's College Hospital NHS Foundation Trust
2019-2021
Third Way
2019
Immungenetics (Germany)
2018
Bilirubin, a breakdown product of heme, is normally glucuronidated and excreted by the liver into bile. Failure this system can lead to buildup conjugated bilirubin in blood, resulting jaundice. The mechanistic basis excretion hyperbilirubinemia syndromes largely understood, but that Rotor syndrome, an autosomal recessive disorder characterized hyperbilirubinemia, coproporphyrinuria, near-absent hepatic uptake anionic diagnostics, has remained enigmatic. Here, we analyzed 8 Rotor-syndrome...
Acetaminophen or paracetamol (APAP) overdose is a common cause of liver injury. Silymarin (SLM) hepatoprotective agent widely used for treating injury different origin. In order to evaluate the possible beneficial effects SLM, Balb/c mice were pretreated with SLM (100 mg/kg b.wt. per os) once daily three days. Two hours after last dose, administered APAP (300 i.p.) and killed 6 (T6), 12 (T12) 24 (T24) later. SLM-treated exhibited significant reduction in APAP-induced injury, assessed...
Nonalcoholic fatty liver disease is associated with chronic oxidative stress. In our study, we explored the antioxidant effect of antidiabetic metformin on [high-fat diet (HFD)-induced] and acute stress induced by short-term warm partial ischemia-reperfusion (I/R) or a combination both in liver. Wistar rats were fed standard (SD) HFD for 10 wk, half them being administered (150 mg·kg body wt(-1)·day(-1)). Metformin treatment prevented stress-induced necroinflammatory reaction, reduced...
Steatosis occurs frequently after liver transplantation (LT). We aimed to determine the prevalence of steatosis in adult LT recipients, effects significant (>33%; grades 2-3) on patient survival, and identify risk factors for development its effect fibrosis progression. retrospectively examined 2360 posttransplant biopsies 548 recipients. Survival was compared between patients with those 0-1 steatosis. Patients were controls without (grade 0) clinical laboratory found 309 (56.4%) patients,...
The most common primary malignancy of the liver, hepatocellular carcinoma (HCC), is a heterogeneous tumor entity with high metastatic potential and complex pathophysiology. Increasing evidence suggests that tissue mechanics plays critical role in onset progression. Here we show plectin, major cytoskeletal crosslinker protein, crucial mechanical homeostasis mechanosensitive oncogenic signaling drives hepatocarcinogenesis. Our expression analyses revealed elevated plectin levels liver tumors,...
Accumulation of extracellular matrix (ECM) in liver fibrosis is associated with changes protein abundance and composition depending upon etiology the underlying disease. Current efforts to unravel etiology-specific mechanisms pharmacological targets rely on several models experimental fibrosis. Here, we characterize compare dynamics hepatic proteome remodeling during development spontaneous healing hepatotoxic (carbon tetrachloride (CCl 4 ) intoxication) cholestatic...
The most common primary malignancy of the liver, hepatocellular carcinoma (HCC), is a heterogeneous tumor entity with high metastatic potential and complex pathophysiology. Increasing evidence suggests that tissue mechanics plays critical role in onset progression. Here, we show plectin, major cytoskeletal crosslinker protein, crucial mechanical homeostasis mechanosensitive oncogenic signaling drives hepatocarcinogenesis. Our expression analyses revealed elevated plectin levels liver tumors,...
Abstract: Spitz tumors represent a heterogeneous group of melanocytic neoplasms with spectrum biological behavior ranging from benign (Spitz nevus) to malignant (spitzoid melanoma). Prediction the these lesions based on their histological presentation is not always possible. Recently, mutually exclusive activating kinase fusions, involving ALK, NTRK1, NTRK3, RET, MET, ROS1, and BRAF, have been found in subset spitzoid lesions. Some genetic alterations were associated specific morphological...
Abstract As a natural polysaccharide polymer, glycogen possesses suitable properties for use as nanoparticle carrier in cancer theranostics. Not only it is inherently biocompatible, can also be easily chemically modified with various moieties. Synthetic conjugates passively accumulate tumours due to enhanced permeability of tumour vessels and limited lymphatic drainage (the EPR effect). For this study, we developed examined glycogen-based containing gadolinium chelate near-infrared...
Plectin, a highly versatile cytolinker protein, provides tissues with mechanical stability through the integration of intermediate filaments (IFs) cell junctions. Here, we hypothesize that plectin-controlled cytoarchitecture is critical determinant intestinal barrier function and homeostasis. Mice lacking plectin in an epithelial (IEC; PleΔIEC) spontaneously developed colitis characterized by extensive detachment IECs from basement membrane (BM), increased permeability, inflammatory lesions....
High plasma concentrations of bile acids (BA) and bilirubin are hallmarks cholestasis. BA implicated in the pathogenesis cholestatic liver damage through mechanisms involving oxidative stress, whereas is a strong antioxidant. We evaluated roles on mediating stress rats following duct ligation (BDL). Adult female Wistar Gunn intraperitoneally anaesthetized with ketamine xylazine underwent BDL or sham operation. Cholestatic markers, antioxidant capacity, lipid peroxidation heme oxygenase (HO)...
The effectiveness of cell transplantation can be improved by optimization the site. For some types cells that form highly oxygen-demanding tissue, e.g., pancreatic islets, a successful engraftment depends on immediate and sufficient blood supply. This critical point avoided when are transplanted into bioengineered pre-vascularized cavity which formed using polymer scaffold. In our study, we tested surface-modified poly(lactide-co-caprolactone) (PLCL) capsular scaffolds containing...