A5055590460- Mitochondrial Function and Pathology
- Adipose Tissue and Metabolism
- Metabolism and Genetic Disorders
- ATP Synthase and ATPases Research
- Muscle metabolism and nutrition
- Metabolism, Diabetes, and Cancer
- Diet and metabolism studies
- Metabolomics and Mass Spectrometry Studies
- Cancer, Hypoxia, and Metabolism
- Cardiac Ischemia and Reperfusion
- Lipid metabolism and biosynthesis
- Peroxisome Proliferator-Activated Receptors
- Cancer-related Molecular Pathways
- Coenzyme Q10 studies and effects
- Liver physiology and pathology
- Pancreatic function and diabetes
- Liver Disease Diagnosis and Treatment
- Neuroscience and Neuropharmacology Research
- Boron Compounds in Chemistry
- Biochemical Acid Research Studies
- Muscle Physiology and Disorders
- Photosynthetic Processes and Mechanisms
- Drug-Induced Hepatotoxicity and Protection
- Pharmacological Effects and Assays
- Cancer Mechanisms and Therapy
Czech Academy of Sciences, Institute of Physiology
2014-2024
Charles University
2009-2023
Czech Academy of Sciences
2013-2023
University of Hradec Králové
2015-2023
University Hospital Hradec Králové
2009
Igenbio (United States)
2004
University of Bari Aldo Moro
1970-1998
Lomonosov Moscow State University
1989
Wenner-Gren Foundations
1972
Medical University of Vienna
1972
Oxidative stress and mitochondrial dysfunction play an important role in the pathogenesis of nonalcoholic fatty liver disease toxic injury. The present study was designed to evaluate effect exogenous inducer oxidative (tert-butyl hydroperoxide, tBHP) on nonfatty steatotic hepatocytes isolated from rats fed by standard high-fat diet, respectively. In control hepatocytes, we found higher generation ROS, increased lipoperoxidation, altered redox state glutathione, decreased ADP-stimulated...
Nonalcoholic fatty liver disease is associated with chronic oxidative stress. In our study, we explored the antioxidant effect of antidiabetic metformin on [high-fat diet (HFD)-induced] and acute stress induced by short-term warm partial ischemia-reperfusion (I/R) or a combination both in liver. Wistar rats were fed standard (SD) HFD for 10 wk, half them being administered (150 mg·kg body wt(-1)·day(-1)). Metformin treatment prevented stress-induced necroinflammatory reaction, reduced...
Abstract Pancreatic cancer is one of the hardest-to-treat types neoplastic diseases. Metformin, a widely prescribed drug against type 2 diabetes mellitus, being trialed as an agent pancreatic cancer, although its efficacy low. With idea delivering metformin to molecular target, mitochondrial complex I (CI), we tagged with vector, triphenylphosphonium group. Mitochondrially targeted (MitoMet) was found kill panel cells three four orders magnitude more efficiently than for parental compound....
Recently, the relationship of mitochondrial DNA (mtDNA) variants to metabolic risk factors for diabetes and other common diseases has begun attract increasing attention. However, progress in this area been limited because (1) phenotypic effects variation genome are difficult isolate owing confounding nuclear genome, imprinting phenomena, environmental factors; (2) few animal models have available directly investigating mtDNA on complex phenotypes vivo. Substitution different genomes same...
The obesogenic effect of a high-fat (HF) diet is counterbalanced by stimulation energy expenditure and lipid oxidation in response to meal. aim this study was reveal whether muscle nonshivering thermogenesis could be stimulated HF diet, especially obesity-resistant A/J compared with obesity-prone C57BL/6J (B/6J) mice. Experiments were performed on male mice born maintained at 30°C. Four-week-old randomly weaned onto low-fat (LF) or for 2 wk. In the LF mice, cold exposure (4°C) resulted...
Epigallocatechin-3-gallate (EGCG) is the main compound of green tea with well-described antioxidant, anti-inflammatory, and tumor-suppressing properties. However, EGCG at high doses was reported to cause liver injury. In this study, we evaluated effect on primary culture rat hepatocytes mitochondria in permeabilized hepatocytes. The 24-hour incubation concentrations 10 μ mol/L higher led signs cellular injury a decrease hepatocyte functions. formation reactive oxygen species (ROS) biphasic....
Respiratory complex II (CII, succinate dehydrogenase, SDH) inhibition can induce cell death, but the mechanistic details need clarification. To elucidate role of reactive oxygen species (ROS) formation upon ubiquinone-binding (Qp) site blockade, we substituted CII subunit C (SDHC) residues lining Qp by site-directed mutagenesis. Cell lines carrying these mutations were characterized on bases activity and exposed to inhibitors MitoVES, thenoyltrifluoroacetone (TTFA) Atpenin A5. We found that...
In this study, we focused on an analysis of biguanides effects mitochondrial enzyme activities, membrane potential and permeability transition pore function. We used phenformin, which is more efficient than metformin, evaluated its effect rat liver mitochondria isolated hepatocytes. contrast to previously published data, found that after a 5 min pre-incubation, dose-dependently inhibits not only complex I but also II IV activity in mitochondria. The enzymes complexes inhibition paralleled by...
Non-shivering thermogenesis (NST) mediated by uncoupling protein 1 (UCP1) in brown adipose tissue (BAT) can be activated via the adrenergic system response to cold or diet, contributing both thermal and energy homeostasis. Other mechanisms, including metabolism of skeletal muscle, may also involved NST. However, relative contribution these dissipating pathways their adaptability remain a matter long-standing controversy.We used warm-acclimated (30 °C) mice characterize effect an up 7-day...
Evidence showing the existence in inner compartment of rat‐heart mitochondria AKAP121 and associated PKA is presented. Immunoblotting analysis trypsin digestion pattern show that 90% or more mitochondrial C‐PKA, R‐PKA localized compartment, when prepared both from isolated cardiomyocyte cultures. This localization verified by measurement specific catalytic activity PKA, radiolabelling 32 P‐phosphorylated C‐PKA AKAP electron microscopy exposed to gold‐conjugated antibody.
Research Article| October 01 1964 Acetoacetate formation by liver slices from adult and infant rats Z Drahota; Drahota Search for other works this author on: This Site PubMed Google Scholar P Hahn; Hahn A Kleinzeller; Kleinzeller Kostolánská Author article information Publisher: Portland Press Ltd © The Biochemical Society1964 Biochem J (1964) 93 (1): 61–65. https://doi.org/10.1042/bj0930061 Views Icon Article contents Figures & tables Video Audio Supplementary Data Peer Review Share...