A5063809380- Receptor Mechanisms and Signaling
- Neuroscience and Neuropharmacology Research
- Neuropeptides and Animal Physiology
- Lipid Membrane Structure and Behavior
- Ion channel regulation and function
- Adipose Tissue and Metabolism
- Mitochondrial Function and Pathology
- Protein Kinase Regulation and GTPase Signaling
- ATP Synthase and ATPases Research
- Caveolin-1 and cellular processes
- Liver Disease and Transplantation
- Ion Transport and Channel Regulation
- Liver Disease Diagnosis and Treatment
- Photosynthetic Processes and Mechanisms
- Cellular transport and secretion
- Sepsis Diagnosis and Treatment
- Nitric Oxide and Endothelin Effects
- Traumatic Brain Injury and Neurovascular Disturbances
- Protein Structure and Dynamics
- Bipolar Disorder and Treatment
- Spacecraft Design and Technology
- Neurobiology and Insect Physiology Research
- Gamma-ray bursts and supernovae
- Electrolyte and hormonal disorders
- Glycosylation and Glycoproteins Research
Czech Academy of Sciences, Institute of Physiology
2015-2024
Czech Academy of Sciences
2015-2024
Czech Aerospace Research Centre
2017-2024
Institute of Molecular Genetics
2019-2024
University Hospital in Motol
2022
Charles University
2002-2022
Technical University of Liberec
2019
Trauma Hospital of Brno
1992-2012
Masaryk University
2010
University Hospital Brno
2008
The expression and release of tissue factor is a major trigger for the activation coagulation in patients with sepsis. Tissue pathway inhibitor (TFPI) forms complex blood protease factors leading to inhibition thrombin generation fibrin formation.To determine if administration tifacogin (recombinant TFPI) provides mortality benefit severe sepsis elevated international normalized ratio (INR) assess safety sepsis, including low INR.A randomized, double-blind, placebo-controlled, multicenter,...
Objective To identify a safe and potentially effective recombinant tissue factor pathway inhibitor (rTFPI) dose for further clinical evaluation in patients with severe sepsis. Design Prospective, randomized, single-blind, placebo-controlled, escalation, multicenter, multinational phase II trial. Setting Thirty-eight intensive care units the United States Europe. Patients Two hundred ten subjects sepsis who received standard supportive antimicrobial therapy. Interventions Subjects continuous...
Abstract Background Ventilator-associated pneumonia caused by Pseudomonas aeruginosa (PA) in hospitalised patients is associated with high mortality. The effectiveness of the bivalent, bispecific mAb MEDI3902 (gremubamab) preventing PA nosocomial was assessed PA-colonised mechanically ventilated subjects. Methods EVADE (NCT02696902) a phase 2, randomised, parallel-group, double-blind, placebo-controlled study Europe, Turkey, Israel, and USA. Subjects ≥ 18 years old, ventilated, tracheally...
The involvement of cytokines in trauma still has not been satisfactorily elucidated. development multiorgan failure, the very serious complication multiple truma with high mortality, should also be controlled by cytoknes, endotoxin other mediators. We therefore prospectively studied 42 consecutive patients traums admitted from June to December 1992 Research Institute for Traumatology and Surgery Brno. Study were characterized Injury Severity Score (ISS), Revised Trauma TRISS methodology. In...
The stimulatory guanine nucleotide-binding protein (Gs), which links cell-surface receptors to second-messenger effector systems, is assumed be confined plasma membranes. In the current studies we tested whether Gs redistributes within cells by treating S49 lymphoma with beta-adrenergic agonist isoproterenol, then separating cytosol and crude membrane fractions (defined as pellet supernatant, respectively, after centrifugation for 1 hr at 150,000 x g), assaying alpha subunit of (alpha s)...
In this study, we focused on an analysis of biguanides effects mitochondrial enzyme activities, membrane potential and permeability transition pore function. We used phenformin, which is more efficient than metformin, evaluated its effect rat liver mitochondria isolated hepatocytes. contrast to previously published data, found that after a 5 min pre-incubation, dose-dependently inhibits not only complex I but also II IV activity in mitochondria. The enzymes complexes inhibition paralleled by...
Current strategies for cell-based screening generally focus on the development of highly specific assays, which require an understanding nature signaling molecules and cellular pathways involved. In contrast, changes in temperature cells provides a measure altered metabolism that is not stimulus hence could have widespread applications receptor agonists antagonists, as well assessment toxicity new lead compounds. Consequently, we developed micromachined nanocalorimetric biological sensor...
The beta-adrenergic receptors of hamster brown adipocytes have been characterised by binding the radioactive ligand (-)-[3H]dihydroalprenolol, directly to isolated intact cells in suspension. fat cell contains 57,000 specific and saturable sites which a dissociation constant (Kd) for [3H]dihydroalprenolol 1.4 nM as determined Scatchard analysis. kinetically derived Kd, from forward reverse rate constants, is 5 nM. Both these values are agreement with (Kd = 2.2 nM) alprenolol, competition...
Cerebral oedema is associated with significant neurological damage in patients traumatic brain injury. Bradykinin an inflammatory mediator that may contribute to cerebral by increasing the permeability of blood-brain barrier. We evaluated safety and effectiveness non-peptide bradykinin B2 receptor antagonist Anatibant treatment During course trial, funding was withdrawn sponsor. Adults injury a Glasgow Coma Scale score 12 or less, who had CT scan showing intracranial abnormality consistent...
Chronic catecholamine stimulation in vivo of brown adipose tissue during acclimation hamsters to cold does not result any alteration beta-adrenergic receptor number or affinity when determined isolated adipocytes by (-)-[3H]dihydroalprenolol binding. Norepinephrine displacement showed the same Ki for both groups. However, slope curve was shallower cells from cold-acclimated animals than controls. Cyclic AMP accumulation stimulated norepinephrine groups animals, although dose-response shifted...
Intra-abdominal pressure (IAP) is an important mechanism stabilizing the spine and trunk. IAP regulation depends on coordination of abdominal muscles, diaphragm pelvic floor muscles.To determine differences in wall tension (AWT) various postural positions, first without any correction, then after verbal manual instructions according to Dynamic Neuromuscular Stabilization (DNS) principles.In a cross-sectional observational study, thirty healthy individuals (mean age = 22.73 ± 1.91 years) were...
Background: The treatment of choice for central venous access device (CVAD) occlusion is intracatheter thrombolysis, which has been reported to reestablish patency in up 80% cases. However, these salient results have only achieved highly selected CVAD subgroups such as nontunneled devices adult patients, with recent occlusion, and partially occluded through fluid can still be infused (withdrawal occlusions). Less known about the success thrombolysis broader range malfunction encountered...