A5025385905- Antibiotic Resistance in Bacteria
- Antimicrobial Resistance in Staphylococcus
- Pneumonia and Respiratory Infections
- Bacterial Identification and Susceptibility Testing
- Enterobacteriaceae and Cronobacter Research
- Streptococcal Infections and Treatments
- Respiratory viral infections research
- Antibiotic Use and Resistance
- SARS-CoV-2 and COVID-19 Research
- Antibiotics Pharmacokinetics and Efficacy
- Nosocomial Infections in ICU
- Bacterial biofilms and quorum sensing
- SARS-CoV-2 detection and testing
- Gut microbiota and health
- Infective Endocarditis Diagnosis and Management
- Respiratory and Cough-Related Research
- Salmonella and Campylobacter epidemiology
- Pharmaceutical and Antibiotic Environmental Impacts
- Vibrio bacteria research studies
- Clostridium difficile and Clostridium perfringens research
- Mycobacterium research and diagnosis
- Escherichia coli research studies
- Biosensors and Analytical Detection
- Urinary Tract Infections Management
- Viral gastroenteritis research and epidemiology
University of Antwerp
2016-2025
Netherlands Comprehensive Cancer Organisation
2025
University Hospital Cologne
2024
Hospital Universitario Virgen Macarena
2024
University of Verona
2024
Clinical Research Consortium
2024
Duke University
2024
Rega Institute for Medical Research
2024
KU Leuven
2024
Antwerp University Hospital
1997-2023
We identified a novel plasmid-mediated colistin-resistance gene in porcine and bovine colistin-resistant Escherichia coli that did not contain mcr-1. The gene, termed mcr-2, 1,617 bp phosphoethanolamine transferase harboured on an IncX4 plasmid, has 76.7% nucleotide identity to Prevalence of mcr-2 E. (11/53) Belgium was higher than mcr-1 (7/53). These data call for immediate introduction screening ongoing molecular epidemiological surveillance Gram-negative pathogens.
ABSTRACT A multiplex PCR for the simultaneous detection of five virulence genes ( asa1 , gelE cylA esp and hyl ) in enterococci was developed. The presence these investigated 153 clinical 118 fecal Enterococcus faecium isolates from inpatients at an increased risk developing infections (such as patients intensive care units hematology wards) 13 hospitals eight European countries. Of 271 E. isolates, 135 were vancomycin resistant (VREF) 136 susceptible (VSEF) isolates. Susceptibilities to...
<b>Objectives</b> To quantify the diagnostic accuracy of selected inflammatory markers in addition to symptoms and signs for predicting pneumonia derive a tool. <b>Design</b> Diagnostic study performed between 2007 2010. Participants had their history taken, underwent physical examination measurement C reactive protein (CRP) procalcitonin venous blood on day they first consulted, chest radiography within seven days. <b>Setting</b> Primary care centres 12 European countries....
ABSTRACT Resistance to macrolides and tetracyclines is increasing among streptococci co-occurs as their resistance determinants are carried on the same mobile element. We developed a multiplex PCR facilitate simultaneous specific detection of for both [ erm (A), (B), mef (A/E)] tet (M), (O), (K), (L)] in streptococci.
To perform the first multinational Enterobacter cloacae clonality study, using MLST scheme newly developed in Japan.The analysis included 195 rectal carriage E. isolates resistant to expanded-spectrum cephalosporins (ESCs), collected from patients 12 hospital units across Europe and Israel. All of were typed by PFGE 173 subjected MLST. ESC resistance was analysed phenotypically; genes encoding ESBLs carbapenemases identified PCR sequencing.MLST distinguished 88 STs, which correlated with...
Laboratory preparedness with quality-assured diagnostic assays is essential for controlling the current coronavirus disease (COVID-19) outbreak. We conducted an external quality assessment study inactivated severe acute respiratory syndrome 2 (SARS-CoV-2) samples to support clinical laboratories a proficiency testing option molecular assays. To analyse SARS-CoV-2 performance, we used online questionnaire developed European Union project RECOVER assess capacities in laboratories.
Antibiotic-induced modulation of the intestinal microbiota can lead to Clostridioides difficile infection (CDI), which is associated with considerable morbidity, mortality, and healthcare-costs globally. Therefore, identification markers predictive CDI could substantially contribute guiding therapy decreasing burden. Here, we analyze hospitalized patients at increased risk in a prospective, 90-day cohort-study before after antibiotic treatment diarrhea onset. We show that developing already...
Abstract It is well established that antibiotic treatment selects for resistance, but the dynamics of this process during infections are poorly understood. Here we map responses Pseudomonas aeruginosa to in high definition a lung infection single ICU patient. Host immunity and therapy with meropenem suppressed P. , second wave emerged due growth oprD wbpM resistant mutants evolved situ. Selection then led loss resistance by decreasing prevalence low fitness mutants, increasing frequency...
Abstract Objectives To determine the susceptibility profiles and resistome of Pseudomonas aeruginosa isolates from European ICUs during a prospective cohort study (ASPIRE-ICU). Methods 723 respiratory samples or perianal swabs 402 patients 29 sites in 11 countries were studied. MICs 12 antibiotics determined by broth microdilution. Horizontally acquired β-lactamases analysed through phenotypic genetic assays. The first 105 providing such WGS, including analysis previously defined genotypic...
Many European countries have recently reported upsurges in invasive group A Streptococcus (iGAS) infections, mainly caused by emm1 pyogenes, specifically the toxigenic M1UK lineage. We present epidemiology of causing iGAS Belgium during 2018-August 2023, and describe an emergence lineage mid-2022 that was observed as increase bloodstream infections S. pyogenes continued into 2023.
Campylobacter spp. are a frequent cause of diarrhoea in man, originating mostly from poultry. It has been suggested that the veterinary use antibiotics is largely responsible for resistance human isolates, particularly to quinolones. During 6 month period June December 1998, 677 isolates were obtained healthy poultry and pigs. Samples taken at Belgian slaughterhouses. Species identification was performed by biochemical tests, multiplex PCR SDS–PAGE whole-cell proteins. The vitro...
Resistance of streptococci to macrolide antibiotics is caused by target-site modification or drug efflux. The phenotypic expression can be inducible constitutive. prevalence the three phenotypes among Belgian erythromycin-resistant Group A (GAS) and Streptococcus pneumoniae isolates was surveyed, their MICs for seven were determined clonality explored. Of 2014 GAS tested 131(6.5%) erythromycin resistant (MIC > 1 mg/L): 110 (84.0%) showed M-resistance phenotype whereas remaining 21 strains...
In 5-40% of respiratory infections in children, the diagnostics remain negative, suggesting that patients might be infected with a yet unknown pathogen. Virus discovery cDNA-AFLP (VIDISCA) is virus method based on recognition restriction enzyme cleavage sites, ligation adaptors and subsequent amplification by PCR. However, direct pathogens nasopharyngeal swabs difficult due to high concentration ribosomal RNA (rRNA) acts as competitor. current study we optimized VIDISCA adjusting reverse...
Susceptibility testing for colistin remains challenging primarily due to its inherent properties. We evaluated stability in agar and reproducibility of MICs obtained by dilution, broth macro- micro-dilution MIC gradient strips on 3-7 iterations each method using clinical Klebsiella pneumoniae (susceptible-CS, resistant-CR, n = 2 each), mcr-harboring Escherichia coli (n 2), reference strains E. ATCC25922 Pseudomonas aeruginosa ATCC27853. were not the given range Etest microdilution...
Abstract Ventilator-associated pneumonia (VAP) is one of the commonest hospital-acquired infections associated with high mortality. VAP pathogenesis closely linked to organisms colonizing endotracheal tube (ETT) such as Staphylococcus epidermidis and Pseudomonas aeruginosa , former a common commensal pathogenic potential latter known pathogen. However, recent gut microbiome studies show that pathogens rarely function alone. Hence, we determined ETT microbial consortium co-colonizing S. or P....
Importance Staphylococcus aureus surgical site infections (SSIs) and bloodstream (BSIs) are important complications of procedures for which prevention remains suboptimal. Contemporary data on the incidence etiologic factors these needed to support development improved preventive strategies. Objectives To assess occurrence postoperative S SSIs BSIs quantify its association with patient-related contextual factors. Design, Setting, Participants This multicenter cohort study assessed patients at...