A5015989733- Microbial Community Ecology and Physiology
- Genomics and Phylogenetic Studies
- Cancer Cells and Metastasis
- Adipose Tissue and Metabolism
- Epigenetics and DNA Methylation
- Protist diversity and phylogeny
- Parasite Biology and Host Interactions
- Adipokines, Inflammation, and Metabolic Diseases
- Enzyme Production and Characterization
- Cancer, Lipids, and Metabolism
- Genetics, Aging, and Longevity in Model Organisms
- RNA Research and Splicing
- Insect symbiosis and bacterial influences
- Marine Invertebrate Physiology and Ecology
- Algal biology and biofuel production
- Genetic factors in colorectal cancer
- Skin and Cellular Biology Research
- Immunotherapy and Immune Responses
- Parasitic Infections and Diagnostics
- Retinal Development and Disorders
- Cancer, Hypoxia, and Metabolism
- Immune Response and Inflammation
- Entomopathogenic Microorganisms in Pest Control
- Insect Resistance and Genetics
- Digestive system and related health
Czech Academy of Sciences, Institute of Molecular Genetics
2014-2023
Czech Academy of Sciences
2013-2023
Charles University
2017
Czech Academy of Sciences, Institute of Physiology
2017
Sapienza University of Rome
2017
University of Chemistry and Technology, Prague
2004-2007
New Huadu Business School
2005
Institute of Chemical Technology
2005
Monash Medical Centre
1985
Nodular melanoma is one of the most life threatening tumors with still poor therapeutic outcome. Similarly to other tumors, permissive microenvironment essential for progression. Features this are arising from molecular crosstalk between cells (MC) and surrounding cell populations in context skin tissue. Here, we study effect on human primary keratinocytes (HPK). Presence MC as an important modulator tumor compare it nonmalignant lowly differentiated also originating neural crest (NCSC)....
Spirulina platensis is a blue-green alga used as dietary supplement because of its hypocholesterolemic properties. Among other bioactive substances, it also rich in tetrapyrrolic compounds closely related to bilirubin molecule, potent antioxidant and anti-proliferative agent. The aim our study was evaluate possible anticancer effects S. platensis-derived tetrapyrroles using an experimental model pancreatic cancer. components [phycocyanobilin (PCB) chlorophyllin, surrogate molecule for...
We report a cluster of genes encoding two monooxygenases (SadA and SadB) one FMN reductase (SadC) that enable Microbacterium sp. strain BR1 other Actinomycetes to inactivate sulfonamide antibiotics. Our results show SadA SadC are responsible for the initial attack molecules resulting in release 4-aminophenol. The latter is further transformed into 1,2,4-trihydroxybenzene by SadB prior mineralization concomitant production biomass. As degradation products lack antibiotic activity, presence...
In models extensively used in studies of aging and extended lifespan, such as C. elegans Drosophila, adult senescence is regulated by gene networks that are likely to be similar ones underlie lifespan extension during dormancy. These include the evolutionarily conserved insulin/IGF, TOR germ line-signaling pathways. Dormancy, also known dauer stage larval worm or diapause fly, triggered adverse environmental conditions, results drastically with negligible senescence. It furthermore...
Animal eyes are morphologically diverse. Their assembly, however, always relies on the same basic principle, i.e., photoreceptors located in vicinity of dark shielding pigment. Cnidaria as likely sister group to Bilateria earliest branching phylum with a well developed visual system. Here, we show that camera-type cubozoan jellyfish, Tripedalia cystophora, use genetic building blocks typical vertebrate eyes, namely, ciliary phototransduction cascade and melanogenic pathway. Our findings...
Rhodobacter capsulatus SB 1003 belongs to the group of purple nonsulfur bacteria. Its genome consists a 3.7-Mb chromosome and 133-kb plasmid. The encodes genes for photosynthesis, nitrogen fixation, utilization xenobiotic organic substrates, synthesis polyhydroxyalkanoates. These features made it favorite research tool studying these processes. Here we report its complete sequence.
<i>Heterorhabditis bacteriophora</i> is an entomopathogenic nematode (EPN) which infects its host by accessing the hemolymph where it releases endosymbiotic bacteria of species <i>Photorhabdus luminescens</i>. We performed a genome-wide transcriptional analysis <i>Drosophila</i> response to EPN infection at time point nematodes reached either via cuticle or gut and had started multiply. Many most strongly induced genes have been implicated in immune...
The oocyte-to-zygote transition entails transforming a highly differentiated oocyte into totipotent blastomeres and represents one of the earliest obstacles that must be successfully hurdled for continued development. Degradation maternal mRNAs, which likely lies at heart this transition, is characterized by from mRNA stability to instability during maturation. Although phosphorylation oocyte-specific RNA-binding protein MSY2 maturation implicated in making mRNAs more susceptible...
Tumor stroma is an active part influencing the biological properties of malignancies via molecular cross-talk. Cancer-associated fibroblasts play a significant role in this interaction. These cells frequently express smooth muscle actin and can be classified as myofibroblasts. The adhesion/growth-regulatory lectin galectin-1 effector for their generation. In our study, we set presence actin-positive cancer-associated relation to endogenous vivo competitor (galectin-3). squamous cell...
Abstract Background information Considering an analogy between wound healing and tumour progression, we studied chemokine cytokine transcription expression in normal fibroblasts by co‐culture situ . Results Whole‐genome transcriptome profiling revealed strong upregulation for the interleukin (IL)‐6, IL‐8 CXCL‐1 vitro co‐cultures of with either or malignant epithelial cells compared to fibroblast cultures. The same ILs/chemokines were distinctly upregulated clinical samples squamous cell...
Chitinase-like proteins (CLPs) of the 18 glycosyl hydrolase family retain structural similarity to chitinases but lack enzymatic activity. Although CLPs are upregulated in several human disorders that affect regenerative and inflammatory processes, very little is known about their normal physiological function. We show an insect CLP (<i>Drosophila</i> imaginal disc growth factor 3, IDGF3) plays immune-protective role during entomopathogenic nematode (EPN) infections. During these...
Abstract Animals sense light primarily by an opsin-based photopigment present in a photoreceptor cell. Cnidaria are arguably the most basal phylum containing well-developed visual system. The evolutionary history of opsins animal kingdom has not yet been resolved. Here, we study evolution genome-wide analysis cubozoan jellyfish Tripedalia cystophora , cnidarian possessing complex lens-containing eyes and minor photoreceptors. A large number opsin genes with distinct tissue- stage-specific...
ABSTRACT The purple nonsulfur photosynthetic bacterium Rhodobacter capsulatus has been extensively studied for its metabolic versatility as well production of a gene transfer agent called RcGTA. Production RcGTA is highest in the stationary phase growth and requires response regulator protein CtrA. CtrA Caulobacter crescentus thoroughly role an essential, master cell cycle. Although R. shares high degree sequence similarity with C. protein, it nonessential clearly plays different this...
Advanced HF (heart failure) is associated with altered substrate metabolism. Whether modification of use improves the course remains unknown. The antihyperglycaemic drug MET (metformin) affects metabolism, and its might be improved outcome in diabetic HF. aim present study was to examine whether would improve cardiac function survival also non-diabetic Volume-overload induced male Wistar rats by creating ACF (aortocaval fistula). Animals were randomized placebo/MET (300 mg·kg(-1) body...
Acquisition of genes by plastid genomes (plastomes) via horizontal gene transfer (HGT) seems to be a rare phenomenon. Here, we report an interesting case HGT revealed sequencing the plastomes eustigmatophyte algae Monodopsis sp. MarTras21 and Vischeria CAUP Q 202. These proved harbour unique cluster six genes, most probably acquired from bacterium phylum Bacteroidetes, with homologues in various bacteria, typically organized conserved uncharacterized putative operon. Sequence analyses...
Abstract In this study, following its isolation from contaminated soil, the genomic sequence of Pseudomonas alcaliphila strain JAB1 (=DSM 26533), a biphenyl-degrading bacterium, is reported and analyzed in relation to extensive degradative capabilities. The P. genome (GenBank accession no. CP016162 ) consists single 5.34 Mbp-long chromosome with GC content 62.5%. Gene function was assigned 3816 4908 predicted genes. harbors bph gene cluster, permitting degradation biphenyl many congeners...
Clinical evidence suggests that healing is faster and almost scarless at an early neonatal age in comparison with adults. In this study, the phenotypes of adult dermal fibroblasts keratinocytes (nestin, smooth muscle actin, keratin types 8, 14 19, fibronectin) were compared. Furthermore, functional assays (proliferation, migration, scratch wound closure) including mutual epithelial‑mesenchymal interactions also performed to complete series experiments. Positivity for nestin α actin was...
Abstract Statins, besides being powerful cholesterol-lowering drugs, also exert potent anti-proliferative activities. However, their anti-cancer efficacy differs among the individual statins. Thus, aim of this study was to identify biological pathways affected by statins in an vitro model human pancreatic cancer. The performed on a cancer cell line MiaPaCa-2, exposed all commercially available (12 μM, 24 h exposure). DNA microarray analysis used determine changes gene expression treated...
Solid tumors, including breast cancer, are characterized by the hypoxic microenvironment, extracellular acidosis, and chemoresistance. Hypoxia marker, carbonic anhydrase IX (CAIX), is a pH regulator providing selective survival advantage to cancer cells through intracellular neutralization while facilitating tumor invasion acidification. The expression of CAIX in patients associated with poor prognosis metastases. Importantly, CAIX-positive regions enriched stem (CSCs). Here we investigated...