A5015662860- Pharmaceutical and Antibiotic Environmental Impacts
- Analytical chemistry methods development
- Chemistry and Chemical Engineering
- Pesticide Residue Analysis and Safety
- ATP Synthase and ATPases Research
- S100 Proteins and Annexins
- Pesticide and Herbicide Environmental Studies
- Antibiotic Resistance in Bacteria
- Environmental Chemistry and Analysis
- Click Chemistry and Applications
- Antibiotics Pharmacokinetics and Efficacy
- Analytical Methods in Pharmaceuticals
- Biosimilars and Bioanalytical Methods
- Tuberculosis Research and Epidemiology
- Pharmaceutical Quality and Counterfeiting
- Gut microbiota and health
- Pharmacogenetics and Drug Metabolism
- thermodynamics and calorimetric analyses
- Immune Response and Inflammation
- Water Treatment and Disinfection
- Chemical Reactions and Isotopes
École Polytechnique Fédérale de Lausanne
2025
Bühlmann (Switzerland)
2023
FHNW University of Applied Sciences and Arts
2011-2017
University of Münster
2011
Sulfonamide antibiotics have a wide application range in human and veterinary medicine. Because they tend to persist the environment, pose potential problems with regard propagation of antibiotic resistance. Here, we identified metabolites formed during degradation sulfamethoxazole other sulfonamides Microbacterium sp. strain BR1. Our experiments showed that proceeded along an unusual pathway initiated by ipso-hydroxylation subsequent fragmentation parent compound. The NADH-dependent...
We report a cluster of genes encoding two monooxygenases (SadA and SadB) one FMN reductase (SadC) that enable Microbacterium sp. strain BR1 other Actinomycetes to inactivate sulfonamide antibiotics. Our results show SadA SadC are responsible for the initial attack molecules resulting in release 4-aminophenol. The latter is further transformed into 1,2,4-trihydroxybenzene by SadB prior mineralization concomitant production biomass. As degradation products lack antibiotic activity, presence...
ABSTRACT In this study, we isolated five strains capable of degrading 14 C-labeled sulfamethoxazole to CO 2 from a membrane bioreactor acclimatized sulfamethoxazole, carbamazepine, and diclofenac. Of these strains, two belonged the phylum Actinobacteria , while three were members Proteobacteria .
Carbon isotope fractionation of sulfamethoxazole (SMX) during biodegradation by Microbacterium sp. strain BR1 (ipso-hydroxylation) and upon direct photolysis was investigated. signatures (δ(13)C) SMX were measured LC-IRMS (liquid chromatography coupled to ratio mass spectrometry). A new method for the metabolite, 3-amino-5-methylisoxazole (3A5MI), established. enrichment factors (ε(C)) -0.6 ± 0.1‰ -2.0 -3.0 0.2‰ photolysis, at pH 7.4 5, respectively. The corresponding apparent kinetic...
Abstract Common inflammatory disorders such as ulcerative colitis and Crohn’s disease are non-invasively diagnosed or monitored by the biomarker calprotectin. However, current quantitative tests for calprotectin antibody-based vary depending on type of antibody assay used. Additionally, binding epitopes applied antibodies not characterized structures most it is unclear if they detect dimer, tetramer, both. Herein, we develop ligands based peptides, that offer advantages homogenous chemical...
Peptide 3 is an 18‐amino acid linear peptide binding with sub‐micromolar affinity to the inflammation marker calprotectin. Its application in point‐of‐care diagnostic assays has shown promising results, yet improving its for calprotectin could facilitate development of sensitive and robust assays. Herein we report a detailed structure‐activity relationship analysis better understand importance each individual amino Moreover, have followed two different approaches, one based on prolonging...