Terry Fei Fan Ng
A5055337033- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Viral Infections and Immunology Research
- Plant Virus Research Studies
- Respiratory viral infections research
- Bacteriophages and microbial interactions
- Virus-based gene therapy research
- Viral Infections and Vectors
- Aquaculture disease management and microbiota
- Genomics and Phylogenetic Studies
- Plant and Fungal Interactions Research
- Virology and Viral Diseases
- Microbial infections and disease research
- Pneumonia and Respiratory Infections
- Polyomavirus and related diseases
- Insect symbiosis and bacterial influences
- Whipple's Disease and Interleukins
- Hepatitis B Virus Studies
- RNA and protein synthesis mechanisms
- Mosquito-borne diseases and control
- Vector-Borne Animal Diseases
- Animal Disease Management and Epidemiology
- Immunodeficiency and Autoimmune Disorders
- Herpesvirus Infections and Treatments
- Poxvirus research and outbreaks
AID Atlanta
2021-2024
University of Georgia
2015-2024
CDC Foundation
2018-2023
Centers for Disease Control and Prevention
2015-2022
National Center for Immunization and Respiratory Diseases
2015-2022
Computer Emergency Response Team
2022
University at Albany, State University of New York
2022
Center for Surveillance, Epidemiology, and Laboratory Services
2019-2021
Atlanta Medical Center
2020
Emory University
2020
ABSTRACT Deep sequencing of untreated sewage provides an opportunity to monitor enteric infections in large populations and for high-throughput viral discovery. A metagenomics analysis purified particles from the United States (San Francisco, CA), Nigeria (Maiduguri), Thailand (Bangkok), Nepal (Kathmandu) revealed sequences related 29 eukaryotic families infecting vertebrates, invertebrates, plants (BLASTx E score, <10 −4 ), including known pathogens (>90% protein identities) numerous...
Abstract Next-generation sequencing (NGS) approaches rapidly produce millions to billions of short reads, which allow pathogen detection and discovery in human clinical, animal environmental samples. A major limitation sequence homology-based identification for highly divergent microorganisms is the length reads generated by most parallel technologies. Short require a high level similarities annotated genes confidently predict gene function or homology. Such recognition homologues can be...
ABSTRACT Bovine respiratory disease (BRD) is a common health problem for both dairy and beef cattle, resulting in significant economic loses. In order to identify viruses associated with BRD, we used metagenomics approach enrich sequence viral nucleic acids the nasal swabs of 50 young cattle symptoms BRD. Following deep sequencing, de novo assembly, translated protein similarity searches, numerous known previously uncharacterized were identified. adenovirus 3, bovine adeno-associated virus,...
Polyomaviruses are a family of DNA tumor viruses that known to infect mammals and birds. To investigate the deeper evolutionary history family, we used combination viral metagenomics, bioinformatics, structural modeling approaches identify characterize polyomavirus sequences associated with fish arthropods. Analyses drawing upon divergent new indicate polyomaviruses have been gradually co-evolving their animal hosts for at least half billion years. Phylogenetic analyses individual genes...
the New York State Department of Health (NYSDOH) notified CDC detection poliovirus type 2 in stool specimens from an unvaccinated immunocompetent young adult Rockland County, York, who was experiencing acute flaccid weakness.The patient initially experienced fever, neck stiffness, gastrointestinal symptoms, and limb hospitalized with possible myelitis (AFM).Vaccine-derived (VDPV2) detected obtained on days 11 12 after initial symptom onset.To date, related Sabin-like polioviruses have been...
Viruses are the most abundant and diverse genetic entities on Earth; however, broad surveys of viral diversity hindered by lack a universal assay for viruses inability to sample sufficient number individual hosts. This study utilized vector-enabled metagenomics (VEM) provide snapshot DNA present in three mosquito samples from San Diego, California. The majority sequences were novel, suggesting that community mosquitoes, as well animal plant hosts they feed on, is highly largely...
ABSTRACT Viral metagenomics, consisting of viral particle purification and shotgun sequencing, is a powerful technique for discovering viruses associated with diseases no definitive etiology, that share limited homology known viruses, or are not culturable. Here we used metagenomics to examine sea turtle fibropapillomatosis (FP), debilitating neoplastic disease affecting turtles worldwide. By means purifying sequencing the community directly from fibropapilloma Florida green turtle, novel...
Significance Knowledge of ancient viruses is limited due to their low concentration and poor preservation in specimens. Using a viral particle-associated nucleic acid enrichment approach, we genetically characterized one complete DNA partial RNA genome from 700-y-old fecal sample preserved ice. reverse genetics, reconstituted the virus, which replicated systemically spread model plant species. Under constant freezing conditions, encapsidated acids may therefore be for centuries. Our finding...
Current knowledge of plant virus diversity is biased towards agents visible and economically important diseases. Less known about viruses that have not caused major diseases in crops, or from native vegetation, which are a reservoir biodiversity can contribute to viral emergence. Discovery these hindered by the traditional approach sampling individual symptomatic plants. Since many damaging transmitted insect vectors, we developed "vector-enabled metagenomics" (VEM) investigate viruses. VEM...
ABSTRACT Hepadnaviruses (hepatitis B viruses [HBVs]) are the only animal that replicate their DNA by reverse transcription of an RNA intermediate. Until recently, known host range hepadnaviruses was limited to mammals and birds. We obtained analyzed first amphibian HBV genome, as well several prototype fish HBVs, which allow comprehensive comparative genomic analysis from four classes vertebrates. Bluegill hepadnavirus (BGHBV) characterized in-house viral metagenomic sequencing. The African...
The presence in cerebrospinal fluid of antibodies to EV peptides at higher levels than non-AFM controls supports the plausibility a link between infection and AFM that warrants further investigation has potential lead strategies for diagnosis prevention disease.
§ A VDPV is a strain related to the attenuated live poliovirus contained in OPV.VDPV2s are OPV virus strains that >0.6%divergent (or at least six nucleotide changes) from OPV2 complete VP1 genomic region.https://polioeradication.org/wp-content/uploads/2016/09/ Reporting-and-Classification-of-VDPVs_Aug2016_EN.pdf ¶ The term PV2, referring all serotype 2 polioviruses, used throughout report indicate either confirmed VDPV2 or type Sabin-like genetically Rockland County patient.A one of Sabin...
Increases in severe respiratory illness and acute flaccid myelitis (AFM) among children adolescents resulting from enterovirus D68 (EV-D68) infections occurred biennially the United States during 2014, 2016, 2018, primarily late summer fall. Although EV-D68 annual trends are not fully understood, levels were lower than expected 2020, potentially because of implementation COVID-19 mitigation measures (e.g., wearing face masks, enhanced hand hygiene, physical distancing) (1). In August 2022,...
A viral metagenomic study was performed to investigate potential pathogens associated with a mortality event of three captive California sea lions ( Zalophus californianus ). This identified novel lion anellovirus (ZcAV), 35 % amino acid identity in the ORF1 region feline anelloviruses. The double-stranded replicative form ZcAV detected lung tissue, suggesting that replicates lungs. Specific PCR revealed presence tissue all involved event, but not other from same zoo. In addition, at low...
As dominant members of marine mesozooplankton communities, copepods play critical roles in oceanic food webs and biogeochemical cycling. Despite the ecological significance copepods, little is known regarding causes copepod mortality, up to 35% total mortality cannot be accounted for by predation alone. Viruses have been established as ecologically important infectious agents oceans; however, viral infection has not investigated communities. Here we used molecular microscopic techniques...
ABSTRACT The poliovirus (PV) is currently targeted for worldwide eradication and containment. Sanger-based sequencing of the viral protein 1 (VP1) capsid region standard method PV surveillance. However, whole-genome sequence sometimes needed higher resolution global In this study, we optimized protocols isolates FTA cards using next-generation (NGS), aiming high coverage, efficiency, throughput. We found that DNase treatment RNA followed by random reverse transcription (RT), amplification,...
Based on active, prospective surveillance of acute respiratory illness through the New Vaccine Surveillance Network, EV-D68 was detected in two (0.8%) patients 2017 and 358 (13.9%) 2018. Detections 2018 peaked September.
To investigate viral pathogens potentially involved in a mortality event of 21 Pacific harbor seals (Phoca vitulina richardsii) California 2000, metagenomics was performed directly on lung samples from five individuals. Metagenomics revealed novel seal anellovirus (SealAV), which clusters phylogenetically with anelloviruses sea lions and domestic cats. Using specific PCR, SealAV identified tissue two animals the 2000 event, as well one 20 examined post-mortem 2008. The identification...
Rats are known as reservoirs and vectors for several zoonotic pathogens. However, information on the viruses shed by urban wild rats that could pose a risk to human health is scare. Here, intestinal contents from 20 Norway (Rattus norvegicus) collected in city of Berlin, Germany, were subjected metagenomic analysis viral nucleic acids. The determined faecal viromes consisted variety unknown viruses, highly variable among individuals. Members families Parvoviridae Picobirnaviridae represented...