A5012316558- HIV Research and Treatment
- Animal Virus Infections Studies
- Viral Infections and Vectors
- Mosquito-borne diseases and control
- Viral Infections and Outbreaks Research
- Virology and Viral Diseases
- HIV/AIDS drug development and treatment
- Virus-based gene therapy research
- Viral gastroenteritis research and epidemiology
- interferon and immune responses
- HIV/AIDS Research and Interventions
- Plant Virus Research Studies
- Hepatitis C virus research
- Vector-Borne Animal Diseases
- Immune Cell Function and Interaction
- Primate Behavior and Ecology
- Herpesvirus Infections and Treatments
- Cytomegalovirus and herpesvirus research
- Parasitic infections in humans and animals
- Viral Infections and Immunology Research
- Animal Disease Management and Epidemiology
- Parvovirus B19 Infection Studies
- Parasite Biology and Host Interactions
- HIV-related health complications and treatments
- Molecular Biology Techniques and Applications
Promega (United States)
2024
University of Wisconsin–Madison
2012-2021
Scripps Research Institute
2018
National Institute Of Hygiene And Epidemiology
2018
The University of Tokyo
2018
Chubu University
2018
University of Veterinary Medicine Hannover, Foundation
2016
Erasmus MC
2016
Utrecht University
2016
Asan Medical Center
2016
Significance Congenital cytomegalovirus (CMV) is the leading infectious cause of childhood hearing loss and brain damage worldwide. Yet, despite its high prevalence ranking as a top priority for vaccine development, immune correlates protection that could guide development remain undefined. Using novel nonhuman primate model congenital CMV transmission, we demonstrate critical role maternal CD4 + T cells in induction protective responses prevent fetal demise. In addition to establishing...
ABSTRACT GB virus B (GBV-B; family Flaviviridae , genus Hepacivirus ) has been studied in New World primates as a model for human hepatitis C infection, but the distribution of GBV-B and its relatives nature remained obscure. Here, we report discovery novel highly divergent GBV-B-like an Old monkey, black-and-white colobus ( Colobus guereza ), Uganda. The new virus, hepacivirus (GHV), clusters phylogenetically with recently described hepaciviruses infecting African bats North American...
High genetic diversity at both inter- and intra-host level are hallmarks of RNA viruses due to the error-prone nature their genome replication. Several groups have evaluated extent viral variability using different virus deep sequencing methods. Although much this effort has been dedicated pathogens that cause chronic infections in humans, few studies investigated arthropod-borne, acute infections.We sequenced complete ten DENV2 isolates from representative classical severe cases sampled a...
ABSTRACT In 49 patients with known Ebola virus disease outcomes during the ongoing outbreak in Sierra Leone, 13 were coinfected immunomodulatory pegivirus GB C (GBV-C). Fifty-three percent of these GBV-C + survived; contrast, only 22% − survived. Both survival and status associated age, older having lower rates intermediate-age (21 to 45 years) highest rate infection. Understanding separate combined effects age on may lead new treatment prevention strategies, perhaps through age-related...
Background Simian hemorrhagic fever virus (SHFV) has caused lethal outbreaks of disease in captive primates, but its distribution wild primates remained obscure. Here, we describe the discovery and genetic characterization by direct pyrosequencing two novel, divergent SHFV variants co-infecting a single male red colobus monkey from Kibale National Park, Uganda. Methodology/Principal Findings The viruses were detected directly blood plasma using pyrosequencing, without prior isolation with...
Simian hemorrhagic fever virus (SHFV) is an arterivirus that causes severe disease in captive macaques. We describe two new SHFV variants subclinically infecting wild African red-tailed guenons (Cercopithecus ascanius). Both are highly divergent from the prototype and sympatric red colobus (Procolobus rufomitratus). All known monophyletic share three open reading frames not present other arteriviruses. Our data suggest a need to modify current classification.
Rapid evolution and high intrahost sequence diversity are hallmarks of human simian immunodeficiency virus (HIV/SIV) infection. Minor viral variants have important implications for drug resistance, receptor tropism, immune evasion. Here, we used ultradeep pyrosequencing to complete HIV/SIV genomes, detecting present at a frequency as low 1%. This approach provides more characterization the population than is possible with conventional methods, revealing low-level resistance previously hidden...
The transmembrane envelope protein gp41 of the human immunodeficiency virus HIV-1 plays an important role during infection allowing fusion viral and cellular membrane. In addition, there is increasing evidence that may contribute to induced by HIV-1. Recombinant a synthetic peptide corresponding highly conserved domain in gp41, immunosuppressive (isu) domain, have been shown inhibit mitogen-induced activation peripheral blood mononuclear cells (PBMCs) increase release IL-6 IL-10 from these...
Hepatitis C virus (HCV) is the leading cause of liver disease worldwide. In this study, we analyzed four treatment-naïve patients infected with subtype 1a and performed Roche/454 pyrosequencing across coding region. We report presence low-level drug resistance mutations that would most likely have been missed using conventional sequencing methods. The approach described here broadly applicable to studies viral diversity could help improve efficacy direct-acting antiviral agents (DAA) in...
Within the Flaviviridae, recently designated genus Pegivirus has expanded greatly due to new discoveries in bats, horses, and rodents. Here we report discovery characterization of three simian pegiviruses (SPgV) that resemble human pegivirus (HPgV) infect red colobus monkeys (Procolobus tephrosceles), red-tailed guenons (Cercopithecus ascanius) an olive baboon (Papio anubis). We have these viruses SPgVkrc, SPgVkrtg SPgVkbab, reflecting their host species' common names, which include...
ABSTRACT Simian hemorrhagic fever virus (SHFV) causes a severe and almost uniformly fatal viral in Asian macaques but is thought to be nonpathogenic for humans. To date, the SHFV life cycle completely uncharacterized on molecular level. Here, we describe first steps of cycle. Our experiments indicate that enters target cells by low-pH-dependent endocytosis. Dynamin inhibitors, chlorpromazine, methyl-β-cyclodextrin, chloroquine, concanamycin A dramatically reduced entry efficiency, whereas...
Key biological properties such as high genetic diversity and evolutionary rate enhance the potential of certain RNA viruses to adapt emerge. Identifying with these in their natural hosts could dramatically improve disease forecasting surveillance. Recently, we discovered two novel members viral family Arteriviridae: simian hemorrhagic fever virus (SHFV)-krc1 SHFV-krc2, infecting a single wild red colobus (Procolobus rufomitratus tephrosceles) Kibale National Park, Uganda. Nearly nothing is...
Within the first three weeks of human immunodeficiency virus (HIV) infection, replication peaks in peripheral blood. Despite critical, causal role determining transmissibility and kinetics progression to acquired immune deficiency syndrome (AIDS), there is limited understanding conditions required transform small localized transmitted founder population into a large heterogeneous systemic infection. Here we show that during hyperacute "pre-peak" phase simian (SIV) infection macaques, high...
Abstract Background African non-human primates (NHPs) are natural hosts for simian immunodeficiency viruses (SIV), the zoonotic transmission of which led to emergence HIV-1 and HIV-2. However, our understanding SIV diversity evolution is limited by incomplete taxonomic geographic sampling NHPs, particularly in East Africa. In this study, we screened blood specimens from nine black-and-white colobus monkeys ( Colobus guereza occidentalis ) Kibale National Park, Uganda, novel SIVs using a...
ABSTRACT Nonhuman primates (NHPs) are a historically important source of zoonotic viruses and gold-standard model for research on many human pathogens. However, with the exception simian immunodeficiency virus (SIV) (family Retroviridae ), blood-borne harbored by these animals in wild remain incompletely characterized. Here, we report discovery characterization two novel pegiviruses Flaviviridae ) arteriviruses Arteriviridae African green monkeys from Zambia (malbroucks [ Chlorocebus...
Since the 1960s, simian hemorrhagic fever virus (SHFV; Nidovirales, Arteriviridae) has caused highly fatal outbreaks of viral in captive Asian macaque colonies. However, source(s) these and natural reservoir(s) this remain obscure. Here we report identification two novel, divergent arteriviruses related to SHFV, Mikumi yellow baboon 1 (MYBV-1) Southwest (SWBV-1), wild baboons, respectively, demonstrate recent transmission SWBV-1 among baboons. These findings extend our knowledge genetic...
A small percentage of human immunodeficiency virus (HIV)-infected people and simian (SIV)-infected macaques control replication without antiretroviral treatment. The major determinant this is host expression certain histocompatibility complex alleles. However, association incompletely penetrant, suggesting that additional loci modify the complex's protective effect. Here, to identify candidate control-modifying loci, we sequence genomes 12 SIV-infected Mauritian cynomolgus experienced...
ABSTRACT Simian hemorrhagic fever (SHF) is lethal for macaques. Based on clinical presentation and serological diagnosis, all reported SHF outbreaks were thought to be caused by different strains of the same virus, simian virus (SHFV; Arteriviridae ). Here we show that in Sukhumi 1964 Alamogordo 1989 not SHFV but two novel divergent arteriviruses. Our results indicate multiple arteriviruses can cause SHF.
A monkey model of human pegivirus infection provides new insights into the biology this virus.
A captive juvenile Bornean orangutan (Pongo pygmaeus) died from an unknown disseminated parasitic infection. Deep sequencing of DNA infected tissues, followed by gene-specific PCR and sequencing, revealed a divergent species within the newly proposed genus Versteria (Cestoda: Taeniidae). may represent previously unrecognized risk to primate health.
ABSTRACT Simian hemorrhagic fever (SHF) is a highly lethal disease in captive macaques. Three distinct arteriviruses are known etiological agents of past SHF epizootics, but only one, simian virus (SHFV), has been isolated cell culture. The natural reservoir(s) the three viruses have yet to be identified, African nonhuman primates suspected. Eleven additional divergent detected recently diverse and apparently healthy cercopithecid monkeys. Here, we report successful isolation MARC-145...