A5044348602- Viral Infections and Vectors
- Mosquito-borne diseases and control
- Vector-borne infectious diseases
- SARS-CoV-2 and COVID-19 Research
- Vector-Borne Animal Diseases
- COVID-19 Clinical Research Studies
- Malaria Research and Control
- SARS-CoV-2 detection and testing
- Insect symbiosis and bacterial influences
- COVID-19 epidemiological studies
- Virology and Viral Diseases
- COVID-19 Impact on Reproduction
- Viral Infections and Outbreaks Research
- Insect and Pesticide Research
- Bacillus and Francisella bacterial research
- Poxvirus research and outbreaks
- Yersinia bacterium, plague, ectoparasites research
- Plant and Fungal Interactions Research
- Herpesvirus Infections and Treatments
- Complement system in diseases
- Evolution and Genetic Dynamics
- Antimicrobial Peptides and Activities
- Vaccine Coverage and Hesitancy
- Monoclonal and Polyclonal Antibodies Research
- Aquatic Ecosystems and Biodiversity
New York State Department of Health
2015-2024
University at Albany, State University of New York
2007-2024
Wadsworth Center
2015-2024
University of Massachusetts Amherst
2021-2023
State Farm (United States)
2004-2023
State University of New York
2023
Public Health Agency of Canada
2022
New York Medical College
2013-2019
Yale University
2019
New York Proton Center
2019
Most of the patients infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mount a humoral immune response to virus within few weeks infection, but duration this and how it correlates clinical outcomes has not been completely characterized. Of particular importance is identification infection that would support public health decision-making on treatment approaches, vaccination strategies, convalescent plasma therapy. While ELISA-based assays detect quantitate antibodies...
Beginning in late 2023, Oropouche virus was identified as the cause of large outbreaks Amazon regions with known endemic transmission and new areas South America Caribbean. The is spread to humans by infected biting midges some mosquito species. Although infection typically causes a self-limited febrile illness, reports two deaths patients vertical associated adverse pregnancy outcomes have raised concerns about threat this human health. In addition approximately 8,000 locally acquired cases...
Abstract West Nile (WN) virus was found throughout New York State in 2000, with the epicenter City and surrounding counties. We tested 3,403 dead birds 9,954 mosquito pools for WN during transmission season. Sixty-three avian species, representing 30 families 14 orders, positive virus. The highest proportion of that American Crows (67% positive, n=907). Eight four genera, were minimum infection rate per 1,000 mosquitoes (MIR) Culex pipiens epicenter: 3.53 entire season 7.49 peak week August...
ABSTRACT The recent outbreaks of West Nile virus (WNV) in the northeastern United States and other regions world have made it essential to develop an efficient protocol for surveillance WNV. In present report, we describe a high-throughput procedure that combines automated RNA extraction, amplification, detection WNV RNA. analyzed 96 samples approximately 4.5 h. A robotic system, ABI Prism 6700 Automated Nucleic Acid workstation, extracted set up reactions real-time reverse transcription...
West Nile virus (WNV) has spread rapidly across North America, resulting in human deaths and the of untold numbers birds, mammals, reptiles. The reached Central America Caribbean may to Hawaii South America. Although tens thousands birds have died, studies some bird species show local declines, few regionwide declines can be attributed WNV. Predicting future impacts WNV on wildlife, pinpointing what drives epidemics, will require substantial additional research into host susceptibility,...
Rapid and accurate diagnosis of infectious agents is essential for patient care, disease control, countermeasure development. The present serologic Zika virus (ZIKV) infection relies mainly on IgM-capture ELISA which confounded with the flaw cross-reactivity among different flaviviruses. In this communication, we report a multiplex microsphere immunoassay (MIA) that captures diagnostic power viral envelope protein (that elicits robust, yet cross-reactive antibodies to other flaviviruses)...
Abstract Passive transfer of antibodies from COVID-19 convalescent patients is being used as an experimental treatment for eligible with SARS-CoV-2 infections. The United States Food and Drug Administration’s (FDA) guidelines plasma initially recommended target antibody titers 160. We evaluated neutralizing in sera recovered using plaque reduction neutralization tests (PRNT) at moderate (PRNT50) high (PRNT90) stringency thresholds. found that activity significantly increased time post...
Coronavirus disease 2019 (COVID-19) is associated with a wide spectrum of presentation, ranging from asymptomatic infection to acute respiratory distress syndrome (ARDS). Paradoxically, direct relationship has been suggested between COVID-19 severity and the levels circulating severe coronavirus 2 (SARS-CoV-2)-specific antibodies, including virus-neutralizing titers. A serological analysis 536 convalescent healthcare workers reveals that SARS-CoV-2-specific antibody are elevated in...
Abstract West Nile (WN) virus was found throughout New York State in 2000, with the epicenter City and surrounding counties. We tested 3,403 dead birds 9,954 mosquito pools for WN during transmission season. Sixty-three avian species, representing 30 families 14 orders, positive virus. The highest proportion of that American Crows (67% positive, n=907). Eight four genera, were minimum infection rate per 1,000 mosquitoes (MIR) Culex pipiens epicenter: 3.53 entire season 7.49 peak week August...
In spring 2002, an intensive avian serosurvey was initiated in Jamaica, Puerto Rico, and Mexico. We collected >1,600 specimens from resident nonresident neotropical migratory birds before their northerly migrations. Plaque reduction neutralization test results indicated specific neutralizing antibodies to West Nile virus 11 species Jamaica.
Ixodes scapularis, the blacklegged tick, is capable of transmitting pathogens that cause Lyme disease (Borrelia burgdorferi), babesiosis (Babesia microti), anaplasmosis (Anaplasma phagocytophilum), and to a lesser extent Powassan encephalitis (deer tick virus [DTV]). These represent significant public health problems, but little known about occurrence co-infection prevalence these in I. scapularis. Here, we used standard PCR pathogen-specific primers estimate infection A. phagocytophilium,...
Abstract Background Deer tick virus, DTV, is a genetically and ecologically distinct lineage of Powassan virus (POWV) also known as II POWV. Human incidence POW encephalitis has increased in the last 15 years potentially due to emergence particularly Hudson Valley New York State. We initiated an extensive sampling campaign determine whether POWV was extant throughout vectors and/or vertebrate hosts. Methods More than 13,000 ticks were collected from hosts or vegetation tested for presence...
Jamestown Canyon virus (JCV) is a mosquito-borne orthobunyavirus in the California serogroup that can cause an acute febrile illness, meningitis, or meningoencephalitis. We describe epidemiologic and clinical features for JCV disease cases occurring United States during 2000–2013. A case of was defined as illness person with laboratory evidence recent infection. During 2000–2013, we identified 31 residents 13 states. The median age 48 years (range, 10–69) 21 (68%) were male. Eleven (35%)...
Powassan virus is an emerging tick-borne of concern for public health, but very little known about its transmission patterns and ecology. Here, we expanded the genomic dataset by sequencing 279 viruses isolated from Ixodes scapularis ticks northeastern United States. Our phylogeographic reconstructions revealed that lineage II was likely introduced or emerged a relict population in Northeast between 1940 1975. Sequences strongly clustered sampling location, suggesting highly focal...
We adapted an indirect immunoglobulin G enzyme-linked immunosorbent assay to facilitate studies of West Nile virus (WNV) and evaluated its application taxonomically diverse avian species. Anti-WNV antibodies were detected in 23 bird species, including many exotic demonstrating value WNV epizootiology.
We analyzed nucleotide sequences from the envelope gene of 11 West Nile (WN) virus strains collected in New York State during 2000 transmission season to determine whether they differed genetically each other and initial strain isolated 1999. The complete genes these were amplified by reverse transcription-polymerase chain reaction. resulting aligned, genetic distances computed, a phylogenetic tree was constructed. Ten (0.7%) 1,503 positions polymorphic one or more sequences. 0.003 less. WN...
To investigate the role of migratory birds in dissemination West Nile virus (WNV), we measured prevalence infectious WNV and specific neutralizing antibodies birds, principally Passeriformes, during spring fall migrations Atlantic Mississippi flyways from 2001-2003. Blood samples were obtained 13,403 representing 133 species. Specific antibody was detected 254 resident 39 species, most commonly northern cardinals (Cardinalis cardinalis) (9.8%, N = 762) gray catbirds (Dumetella carolinensis)...
Shallow, polymictic lakes with low heat storage capacity are especially susceptible to warmer spring conditions, predicted for a changing climate. In these lakes, atmosphere water mass transfer is efficient as result of high wind exposures and large surface areas relative volumes. We examined effects temperatures on phytoplankton zooplankton abundance species composition in three small eutrophic (5.2–14.9 ha) the Canadian prairies Winnipeg, Manitoba, Canada, over two open-water seasons...
Powassan virus, a member of the tick-borne encephalitis group flaviviruses, encompasses 2 lineages with separate enzootic cycles. The prototype lineage virus (POWV) is principally maintained between Ixodes cookei ticks and groundhog (Marmota momax) or striped skunk (Mephitis mephitis), whereas deer tick (DTV) believed to be scapularis white-footed mouse (Peromyscus leucopus). We report 14 cases from New York during 2004-2012. Ten (72%) patients were residents Lower Hudson Valley, Lyme...
The potential association of microcephaly and other congenital abnormalities with Zika virus (ZIKV) infection during pregnancy underlines the critical need for a rapid accurate diagnosis. Due to short duration ZIKV viremia in infected patients, serologic assay that detects antibody responses viral plays an essential role diagnosing patient specimens. current diagnosis relies heavily on labor-intensive Plaque Reduction Neutralization Test (PRNT) requires more than one-week turnaround time...
SeroNet institutions have developed or implemented 61 diverse COVID-19 serological assays and are collaboratively working to harmonize these using reference materials establish standardized reporting units. This will facilitate clinical interpretation of serology results cross-comparison research data.
Abstract We analyzed nucleotide sequences from the envelope gene of 11 West Nile (WN) virus strains collected in New York State during 2000 transmission season to determine whether they differed genetically each other and initial strain isolated 1999. The complete genes these were amplified by reverse transcription-polymerase chain reaction. resulting aligned, genetic distances computed, a phylogenetic tree was constructed. Ten (0.7%) 1,503 positions polymorphic one or more sequences. 0.003...
ABSTRACT The recent outbreaks of West Nile virus (WNV) infection in the northeastern United States and other regions world have made it essential to develop efficient, sensitive, rapid protocols for surveillance. Laboratory testing is backbone any surveillance program. Protocols detect presence WNV been refined since 1999 sensitivity, speed, efficiency, specificity. This paper presents currently used by New York State Department Health handle vertebrate mosquito specimens that submitted...