A5080678889- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Immunotherapy and Immune Responses
- COVID-19 and healthcare impacts
- Long-Term Effects of COVID-19
- T-cell and B-cell Immunology
- Advanced Proteomics Techniques and Applications
- Cancer Immunotherapy and Biomarkers
- Acute Myocardial Infarction Research
- Viral Infections and Outbreaks Research
- Vaccine Coverage and Hesitancy
- SARS-CoV-2 detection and testing
- Cardiovascular Effects of Exercise
- Microfluidic and Bio-sensing Technologies
- Infection Control and Ventilation
- COVID-19 epidemiological studies
- Microfluidic and Capillary Electrophoresis Applications
- Diabetes and associated disorders
- vaccines and immunoinformatics approaches
- 3D Printing in Biomedical Research
- Immunodeficiency and Autoimmune Disorders
- Molecular Biology Techniques and Applications
- Immune responses and vaccinations
- Mass Spectrometry Techniques and Applications
- Antibiotics Pharmacokinetics and Efficacy
Cedars-Sinai Medical Center
2015-2024
University of Washington
2007-2010
Cleveland Clinic
2010
University of Washington Medical Center
2009
Abstract In a cohort of BNT162b2 (Pfizer–BioNTech) mRNA vaccine recipients ( n = 1,090), we observed that spike-specific IgG antibody levels and ACE2 binding inhibition responses elicited by single dose in individuals with prior SARS-CoV-2 infection 35) were similar to those seen after two doses without 228). Post-vaccine symptoms more prominent for the first dose, but symptomology was between groups second dose.
BACKGROUND. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused more than 1 million deaths worldwide; thus, there is an urgent need to develop preventive and therapeutic strategies. The antituberculosis vaccine bacillus Calmette-Guérin (BCG) demonstrates nonspecific, protective innate immune–boosting effects. Here, we determined whether a history of BCG vaccination was associated with decreased SARS-CoV-2 infection seroconversion in longitudinal, retrospective...
Pronounced sex differences in the susceptibility and response to SARS-CoV-2 infection remain poorly understood. Emerging evidence has highlighted potential importance of autoimmune activation modulating acute recovery trajectories following exposure. Given that immune-inflammatory activity can be sex-biased setting severe COVID-19 illness, aim study was examine sex-specific reactivity absence extreme clinical disease.In this study, we assessed autoantibody (AAB) 91 autoantigens previously...
Importance Some individuals who were infected by the SARS-CoV-2 Omicron variant may have been completely unaware of their infectious status while virus was actively transmissible. Objective To examine awareness among during recent surge in a diverse and populous urban region Los Angeles County. Design, Setting, Participants This cohort study analyzed records adult employees patients an academic medical center enrolled longitudinal COVID-19 serological County, California. These participants...
Sample preparation for protein quantification by mass spectrometry requires multiple processing steps including denaturation, reduction, alkylation, protease digestion, and peptide cleanup. Scaling these procedures the analysis of numerous complex biological samples can be tedious time-consuming, as there are many liquid transfer timed reactions where technical variations introduced propagated. We established an automated sample workflow with a total time 96 5 h, 2 h incubation trypsin....
In the United States, minimum standards for quality control (QC) are specified in federal law under Clinical Laboratory Improvement Amendment and its revisions. Beyond meeting this required standard, laboratories have flexibility to determine their overall QC program.We surveyed chemistry immunochemistry procedures at 21 clinical within leading academic medical centers assess if standardized practices exist testing.We observed significant variation unexpected similarities practice across...
Lay Summary T-cell and antibody responses to severe acute respiratory syndrome coronavirus 2 vaccination in inflammatory bowel disease patients are poorly correlated. preserved by most biologic therapies, but augmented anti-tumor necrosis factor (anti-TNF) treatment. While anti-TNF therapy blunts the response, cellular immunity after is robust.
Objectives We sought to understand the demographic and clinical factors associated with variations in longitudinal antibody response following completion of two-dose regiment BNT162b2 vaccination. Design This study is a 10-month cohort healthcare workers serially measured anti-spike protein IgG (IgG-S) levels using mixed linear models examine their associations participant characteristics. Setting A large, multisite academic medical centre Southern California, USA. Participants total 843 met...
We sought to determine the extent of SARS-CoV-2 seroprevalence and factors associated with across a diverse cohort healthcare workers.
ABSTRACT The double dose regimen for mRNA vaccines against SARS-CoV-2 presents both a hope and challenge global efforts to curb the COVID-19 pandemic. With supply chain logistics impacting rollout of population-scale vaccination programs, increasing attention has turned potential efficacy single versus vaccine administration select individuals. To this end, we examined response Pfizer-BioNTech in large cohort healthcare workers including those with without prior infection. For all...
Currently, 3 vaccines have been granted Emergency Use Authorization for coronavirus disease 2019 (COVID-19) prevention in the United States. These include messenger RNA (mRNA) platform (mRNA-1273; Moderna/National Institutes of Health) and BNT162b2 (Pfizer-BioNTech) an adenovirus vector vaccine (Ad26.CoV2.S; Johnson & Johnson), which were 94%, 95%, 67% effective against COVID-19 infection their phase III registry trials endemic variants at time, respectively.1Kappelman MD, et al....
SeroNet institutions have developed or implemented 61 diverse COVID-19 serological assays and are collaboratively working to harmonize these using reference materials establish standardized reporting units. This will facilitate clinical interpretation of serology results cross-comparison research data.
Abstract Longitudinal studies of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccine-induced immune responses in patients with cancer are needed to optimize clinical care. In a prospective cohort study 366 (291 vaccinated) patients, we measured antibody levels [anti-spike (IgG-(S-RBD) and anti-nucleocapsid immunoglobulin] at three time points. Antibody level trajectories frequency breakthrough infections were evaluated by tumor type timing treatment relative vaccination....
The enhanced precision and selectivity of liquid chromatography-tandem mass spectrometry (LC-MS/MS) makes it an attractive alternative to certain clinical immunoassays. Easily transferrable work flows could help facilitate harmonization ensure high-quality patient care. We aimed evaluate the interlaboratory comparability antibody-free multiplexed insulin C-peptide LC-MS/MS measurements.
Abstract Background Individuals with post-acute sequelae of COVID (PASC) may have a persistence in immune activation that differentiates them from individuals who recovered without clinical sequelae. To investigate how humoral vary this regard, we compared patterns vaccine-provoked serological response patients PASC to prior PASC. Methods We prospectively studied 245 adults clinically diagnosed and 86 successfully COVID. All participants had measures immunity SARS-CoV-2 assayed before or...
Leflunomide (Arava), a drug with immunosuppressive and antiviral effects, is being used in renal transplant recipients, primarily for its action against BK polyomavirus (BKV), which affects 1% to 10% of recipients often causes failure grafted kidneys. effects are solely due an active metabolite, teriflunomide (formerly A77 1726). Trough blood concentrations exceeding 40 microg/mL (148 micromol/L) associated progressive clearance BKV. Toxic become increasingly apparent at higher...
Patient A was a 68-year-old woman with history of lymphoplasmacytic lymphoma who presented after several weeks mucosal bleeding. On admission, her cancer involved 95% bone marrow cells. She pancytopenic and febrile (38.3 °C). Leukocyte count 0.39 × 103/μL (neutrophils, 0.03 103/μL), hematocrit 25%, platelet 21 103/μL, relative serum viscosity 2.3 (reference interval, 1.4–1.8), blood urea nitrogen 6.4 mmol/L (18 mg/dL), creatinine 62 μmol/L (0.7 estimated glomerular filtration rate >60...
T-cells specifically bind antigens to induce adaptive immune responses using highly specific molecular recognition, and a diverse T-cell repertoire with expansion of antigen-specific clones can indicate robust after infection or vaccination. For patients inflammatory bowel disease (IBD), spectrum chronic intestinal diseases usually requiring immunomodulatory treatment, the response has not been well characterized. Understanding patient factors that result in strong vaccination is critical...