A5042473559- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- SARS-CoV-2 and COVID-19 Research
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- Immunodeficiency and Autoimmune Disorders
- Immune Response and Inflammation
- Bacillus and Francisella bacterial research
- Biosensors and Analytical Detection
- COVID-19 Impact on Reproduction
- Viral gastroenteritis research and epidemiology
- Poxvirus research and outbreaks
- Viral Infections and Vectors
- Viral Infections and Immunology Research
- Hepatitis B Virus Studies
- CAR-T cell therapy research
- Mosquito-borne diseases and control
- Respiratory viral infections research
- Viral Infections and Outbreaks Research
- Herpesvirus Infections and Treatments
- Virology and Viral Diseases
- Lipid Membrane Structure and Behavior
- Kawasaki Disease and Coronary Complications
Wadsworth Center
2014-2025
University at Albany, State University of New York
2012-2025
New York State Department of Health
2014-2025
Royal Stoke University Hospital
2024
Keimyung University
2017
Carilion Clinic
2016
Scripps Research Institute
1997
The University of Texas Southwestern Medical Center
1990-1996
University of California, Los Angeles
1993
Children's Hospital of Los Angeles
1993
CD4+ murine T cell clones, TH1 and TH2, can be distinguished by both functional responses their patterns of lymphokine secretion. Recently, a mAb, 23G2, which reacts with subset CD45 molecules (CD45R), has been reported to bind differentially clones TH2 cells. In the present study, normal splenic cells were analyzed for differences in 23G2-reactivity separated into two populations based on density CD45R (CD45Rhi CD45Rlo). The CD45Rhi secrete more IL-2 than IL-4 after stimulation vitro;...
Abstract During activation in vivo, naive CD4+ T cells are exposed to various endogenous ligands, such as cytokines and the neurotransmitter norepinephrine (NE). To determine whether NE affects cell differentiation, we used sort-purified from either BALB/c or DO11.10 TCR-transgenic mouse spleens activated these with anti-CD3/anti-CD28 mAbs APC OVA323–329 peptide, respectively, under Th1-promoting conditions. RT-PCR functional assays using selective adrenergic receptor (AR) subtype...
Abstract Passive transfer of antibodies from COVID-19 convalescent patients is being used as an experimental treatment for eligible with SARS-CoV-2 infections. The United States Food and Drug Administration’s (FDA) guidelines plasma initially recommended target antibody titers 160. We evaluated neutralizing in sera recovered using plaque reduction neutralization tests (PRNT) at moderate (PRNT50) high (PRNT90) stringency thresholds. found that activity significantly increased time post...
Coronavirus disease 2019 (COVID-19) is associated with a wide spectrum of presentation, ranging from asymptomatic infection to acute respiratory distress syndrome (ARDS). Paradoxically, direct relationship has been suggested between COVID-19 severity and the levels circulating severe coronavirus 2 (SARS-CoV-2)-specific antibodies, including virus-neutralizing titers. A serological analysis 536 convalescent healthcare workers reveals that SARS-CoV-2-specific antibody are elevated in...
A rat hybridoma producing a high-affinity IgG2a monoclonal antibody (B3B4) directed against the murine lymphocyte IgE receptor (Fc epsilon R) was established by using purified Fc R from R+ B cells as immunogen. The and polyclonal anti-Fc inhibited binding of to were also used isolate R. B3B4 specifically recognized only 49-Kd on determined immunoprecipitation SDS-PAGE analysis. In addition its reaction with intact R, fragments that present in culture media cells. predominant isolated 38 Kd...
Abstract Culture of murine splenic B cells with interleukin 4 (IL-4) caused the up-regulation lymphocyte Fc receptor for immunoglobulin E (IgE) (Fc epsilon R) over a similar dose range as required Ia up-regulation. However, expression level G gamma did not increase, rather IL-4 slight but consistent decrease in R on cells. R+ hybridoma also responded to by exhibiting increased expression; levels were unaffected. an increase number per cell and highest obtained having both IgE present...
To study the relationship between primary structure of c-myc protein and some its functional properties, we made in-frame insertion deletion mutants normal human coding domain that was expressed from a retroviral promoter-enhancer. We assessed effects these mutations on ability to cotransform rat embryo cells with mutant ras gene, induce foci in Rat-1-derived cell line (Rat-1a), localize nuclei. Using cotransformation assay, found two regions (amino acids 105 143 321 439) where integrity...
We have used staphylococcal enterotoxin B (SEB) to study the role of naive and memory T cells in induction peripheral tolerance. After administration SEB mice, numbers increase, as does proportion cells, which are unresponsive further stimulation with vitro. In addition, generated response conventional antigen, proliferate provide help presence fail respond superantigen. Hence, general, anergized by SEB. These results suggest that SEB-induced activation anergy reflect combined responses...
SeroNet institutions have developed or implemented 61 diverse COVID-19 serological assays and are collaboratively working to harmonize these using reference materials establish standardized reporting units. This will facilitate clinical interpretation of serology results cross-comparison research data.
In 2022 the World Health Organization declared a Public Emergency for an outbreak of mpox, zoonotic Orthopoxvirus (OPV) affecting at least 104 nonendemic locations worldwide. Serologic detection mpox infection is problematic, however, due to considerable antigenic and serologic cross-reactivity among OPVs smallpox-vaccinated individuals. this report, we developed high-throughput multiplex microsphere immunoassay using combination mpox-specific peptides cross-reactive OPV proteins that...
Abstract Peptide Ag initiates CD4+ T cell proliferation, but the subsequent effects of on clonal expansion are not fully known. In this study, murine cells were labeled with fluorescent dye CFSE and stimulated specific peptide Ag. Activation occurred, as CFSE-associated fluorescence was reduced 2-fold each division. Separation proliferating based upon intensity showed that daughter from division proliferate even after removal A limited exposure (∼2 h) to programmed independently Although...
Numerous studies have shown that memory T cell development is Ag dependent and specific. In the present study, we show responses can be made against an to which there has been no prior exposure. unimmunized DO11.10 mice, carry alpha beta transgenes encode a TCR specific for OVA, CD45RB(low) cells express transgenic TCR. These stimulated by OVA proliferate perform typical functions, such as secrete diverse lymphokines provide cognate help B cells, despite fact mice were never exposed OVA....
Rodent B cells respond to culture with IgE by increasing their IgE-specific Fc receptors (Fc epsilon R). The mechanism of this upregulation was characterized on R+ murine cell hybridoma lines. Measurements [35S]methionine incorporated into the R over time indicated that did not appreciably increase rate synthesis. In contrast analysis decay from surface radioiodinated demonstrated acted slow degradation. Very little endocytosis monomeric seen; this, combined observation lysomotropic agents...
JYNNEOS, a third-generation smallpox vaccine, is integral to monkeypox virus (MPXV) control efforts, but the durability of this modified vaccinia Ankara-Bavarian Nordic (MVA-BN) vaccine's effectiveness undefined. We optimized and used plaque reduction neutralization test (PRNT) with authentic clade IIa MPXV assess antibody responses over 12 months in 8 donors vaccinated 2 doses JYNNEOS. One donor previously received ACAM2000 vaccine; 7 were vaccine-naive. IgG (L1, B5, A33) or (E8, H3, A35)...
The development of new point-of-care diagnostic testing tools for the detection infectious diseases such as COVID-19 are a key aspect clinical care and research. Accurate predictive classification methods required to correctly identify treat patients. Here, onset multisystem inflammatory syndrome in children (MIS-C), more serious form COVID-19, was predicted pediatric population using set multivariate immunological biomarker expression values. A first-stage bivariate statistically...
The rising demand for ethical and faith-based investment products in the United States has prompted increased interest Sharia-compliant funds. These funds seek to align financial returns with Islamic principles, including prohibition of (riba), avoidance excessive uncertainty (gharar), halal sectors. However, integrating such models within U.S. system presents regulatory challenges, particularly under oversight Securities Exchange Commission (SEC). This study examines how navigate frameworks...
Infections with West Nile virus (WNV) are typically asymptomatic, but some patients experience severe neurological disease and even death. Over 1500 fatalities have resulted from the more than 37,000 WNV cases in USA between 1999 2012. While it is clear that age a significant risk factor, markers of immune status associated susceptibility to infections incompletely defined. We taken advantage stable characteristics individual profile stratified cohort healthy subjects history asymptomatic or...