A5009072627- Viral gastroenteritis research and epidemiology
- Viral Infections and Immunology Research
- Respiratory viral infections research
- Virus-based gene therapy research
- Animal Virus Infections Studies
- Listeria monocytogenes in Food Safety
- Parvovirus B19 Infection Studies
- Plant Virus Research Studies
- Animal Disease Management and Epidemiology
- Fecal contamination and water quality
- Salmonella and Campylobacter epidemiology
- Pharmaceutical and Antibiotic Environmental Impacts
- RNA and protein synthesis mechanisms
- Identification and Quantification in Food
- Food Safety and Hygiene
- Biomedical and Engineering Education
- Transgenic Plants and Applications
- Microbial infections and disease research
- Pneumonia and Respiratory Infections
- Antibiotic Resistance in Bacteria
- Bacterial Infections and Vaccines
- Advanced Thermodynamic Systems and Engines
- Infection Control and Ventilation
- Plant tissue culture and regeneration
- Viral Infections and Outbreaks Research
National Center for Immunization and Respiratory Diseases
2008-2024
Centers for Disease Control and Prevention
2013-2024
Government of the United States of America
2023
Texas A&M University
2003-2005
Noroviruses are the leading cause of epidemic acute gastroenteritis in United States. From September 2009 through August 2013, 3,960 norovirus outbreaks were reported to CaliciNet. Of 2,895 with a known transmission route, person-to-person and food-borne transmissions for 2,425 (83.7%) 465 (16.1%) outbreaks, respectively. A total 2,475 (62.5%) occurred long-term care facilities (LTCF), 389 (9.8%) restaurants, 227 (5.7%) schools. 435 (11%) typed as genogroup I (GI) 3,525 (89%) GII...
CaliciNet, the outbreak surveillance network for noroviruses in United States, was launched March 2009. As of January 2011, twenty state and local health laboratories had been certified to submit norovirus sequences epidemiologic data CaliciNet. During network's first year, 552 outbreaks were submitted which 78 (14%) associated with foodborne transmission. A total 395 (72%) typed as GII.4, 298 (75%) belonged a new variant, GII.4 New Orleans, emerged October Analysis complete capsid P2 region...
During October-November 2021, clinicians at a children's hospital in Alabama identified five pediatric patients with severe hepatitis and adenovirus viremia upon admission. In November clinicians, the Department of Public Health, Jefferson County CDC began an investigation. This activity was reviewed by conducted consistent applicable federal law policy.
Increases in severe respiratory illness and acute flaccid myelitis (AFM) among children adolescents resulting from enterovirus D68 (EV-D68) infections occurred biennially the United States during 2014, 2016, 2018, primarily late summer fall. Although EV-D68 annual trends are not fully understood, levels were lower than expected 2020, potentially because of implementation COVID-19 mitigation measures (e.g., wearing face masks, enhanced hand hygiene, physical distancing) (1). In August 2022,...
Abstract Municipal and agricultural pollution affects the Rio Grande, a river that separates United States from Mexico. Three hundred twenty-two Escherichia coli isolates were examined for multiple antibiotic resistance phenotypes prevalence of class 1 2 integron sequences. Thirty-two (10%) resistant to antibiotics. Four (13%) these contained 1–specific sequences; one isolate integron–specific Sequencing showed integron–bearing strain two distinct gene cassettes, sat-1 aadA. Although three...
In October 2009, a novel GII.12 norovirus strain emerged in the United States and caused 16% of all reported outbreaks during winter season. Sequence analysis demonstrated recombinant virus with P2 region that was largely conserved compared previously sequenced strains.
Viral metagenomics sequencing of fecal samples from outbreaks acute gastroenteritis the US revealed presence small circular ssDNA viral genomes encoding a replication initiator protein (Rep). were ∼2.5 kb in length, with bi-directionally oriented Rep and capsid (Cap) genes stem loop structure downstream Rep. Several showed evidence recombination. By digital screening an in-house virome database (1.04 billion reads) using BLAST, we identified closely related sequences cases unexplained...
ABSTRACT With poliovirus eradication nearing, few pockets of active wild (WPV) transmission remain in the world. Intratypic differentiation (ITD) plays a crucial part laboratory surveillance as molecular detection method that can identify and distinguish vaccine-like polioviruses isolated from acute flaccid paralysis cases or environmental sources. The need to detect new variants WPV serotype 1 (WPV1) containment all 2 (PV2) 2015 required changes previous version method. ITD 5.0 is set six...
On April 21, 2022, CDC issued a health advisory† encouraging U.S. clinicians to report all patients aged <10 years with hepatitis of unknown etiology public authorities, after identification similar cases in both the United States (1) and Europe.§ A high proportion initially reported had adenovirus detected whole blood specimens, thus advisory encouraged consider requesting testing, preferentially on specimens. For meeting criteria (patients under investigation [PUIs]), jurisdictional...
Noroviruses are the leading cause of acute gastroenteritis outbreaks worldwide. The majority norovirus caused by genogroup II.4 (GII.4). Novel GII.4 strains emerge every 2 to 4 years and replace older variants as dominant norovirus. through a combination recombination, genetic drift, selection driven population immunity, but exact mechanism how or where is not known. We detected two previously unknown novel variants, termed UNK1 UNK2, diverse in fecal specimens from immunocompromised...
Surveillance and detection of polioviruses (PV) remain crucial to monitoring eradication progress. Intratypic differentiation (ITD) using the real-time RT-PCR kit is key surveillance workflow, where viruses are screened after cell culture isolation before a subset verified by sequencing. The ITD series assays that screens cytopathic effect (CPE)-positive cultures standard WHO method for virus isolation. Because screening critical procedure in poliovirus identification validation performance...
Polioviruses (PV), the main causative agent of acute flaccid paralysis (AFP), are positive-sense single-stranded RNA viruses family Picornaviridae. As we approach polio eradication, accurate and timely detection poliovirus in stool from AFP cases becomes vital to success for eradication efforts. Direct PV clinical diagnostic samples using nucleic acid (NA) extraction real-time reverse transcriptase polymerase chain reaction (rRT-PCR) instead current standard method virus isolation culture,...
We report an increase in the proportion of genotype GI.6 norovirus outbreaks United States from 1.4% 2010 to 7.7% 2012 (p<0.001). Compared with non-GI.6 outbreaks, were characterized by summer seasonality, foodborne transmission, and non-health care settings.