A5023487799- Viral Infections and Immunology Research
- Viral gastroenteritis research and epidemiology
- Respiratory viral infections research
- Bacteriophages and microbial interactions
- Animal Disease Management and Epidemiology
- Animal Virus Infections Studies
- Virus-based gene therapy research
Centers for Disease Control and Prevention
2021-2024
National Center for Immunization and Respiratory Diseases
2021-2024
Government of the United States of America
2023
Surveillance and detection of polioviruses (PV) remain crucial to monitoring eradication progress. Intratypic differentiation (ITD) using the real-time RT-PCR kit is key surveillance workflow, where viruses are screened after cell culture isolation before a subset verified by sequencing. The ITD series assays that screens cytopathic effect (CPE)-positive cultures standard WHO method for virus isolation. Because screening critical procedure in poliovirus identification validation performance...
Polioviruses (PV), the main causative agent of acute flaccid paralysis (AFP), are positive-sense single-stranded RNA viruses family Picornaviridae. As we approach polio eradication, accurate and timely detection poliovirus in stool from AFP cases becomes vital to success for eradication efforts. Direct PV clinical diagnostic samples using nucleic acid (NA) extraction real-time reverse transcriptase polymerase chain reaction (rRT-PCR) instead current standard method virus isolation culture,...
The GPLN is a global surveillance system composed of 146 laboratories in 92 countries, each the six World Health Organization regions. Laboratory for PV relies on virus isolation by cell culture to identify stool samples from AFP cases.
Acute flaccid paralysis (AFP) surveillance has been used to identify polio cases and target vaccination campaigns since the inception of Global Poliovirus Eradication Initiative (GPEI) in 1988. To date, only Afghanistan Pakistan have failed interrupt wild poliovirus transmission. Circulation vaccine-derived polioviruses (VDPV) continues be a problem high-risk areas Eastern Mediterranean, African, Southeast Asian regions. Environmental (ES) is an important adjunct AFP surveillance, helping...
Polioviruses are positive-sense, single-stranded RNA picornaviruses and the principal cause of poliomyelitis. Global poliovirus surveillance has relied on isolation in cells, which may take a minimum 10 days, involves maintaining two cell lines, propagates virus high titers. With eradication underway, major objective Polio Eradication Initiative (GPEI) is to develop culture-independent detection polioviruses as an alternative method complement current technique. A poliovirus-positive stool...
The Global Specialized Polio Laboratory at CDC supports the Poliovirus Network with environmental surveillance (ES) to detect presence of vaccine strain polioviruses, vaccine-derived and wild polioviruses in high-risk countries. Environmental sampling provides valuable supplementary information, particularly areas gaps acute flaccid paralysis (AFP) mainly children less than 15 years. In collaboration Guatemala’s National Health (Laboratorio Nacional de Salud Guatemala), monthly sewage...
In the Global Polio Laboratory Network (GPLN), poliovirus (PV) screening results from acute flaccid paralysis (AFP) surveillance is based on virus isolation (VI) through cell culture, entailing long turnaround times and amplification of live poliovirus. An alternative Direct Detection strategy (DD-ITD) for viral nucleic acid stools, bypassing need has been developed extensively validated by GPLN partners. A multi-laboratory demonstration project was conceived to field-test DD-ITD method...
Enteroviruses can cause human infectious disease. We report 16 near-complete genome sequences of enteroviruses that were isolated through environmental surveillance wastewater in Guatemala.
Polioviruses (PV), the main causative agent of acute flaccid paralysis (AFP), are positive-sense single-stranded RNA viruses family Picornaviridae. As we approach global polio eradication, accurate and timely detection poliovirus in stool from AFP cases becomes vital to success for these efforts. Direct PV clinical diagnostic samples using nucleic acid (NA) extraction real-time reverse transcriptase polymerase chain reaction (rRT-PCR) instead current standard method virus isolation culture,...