A5093559406- Viral gastroenteritis research and epidemiology
- Viral Infections and Immunology Research
- Animal Virus Infections Studies
- Respiratory viral infections research
Centers for Disease Control and Prevention
2023-2024
Cherokee Nation
2024
Government of the United States of America
2023
Polioviruses (PV), the main causative agent of acute flaccid paralysis (AFP), are positive-sense single-stranded RNA viruses family Picornaviridae. As we approach polio eradication, accurate and timely detection poliovirus in stool from AFP cases becomes vital to success for eradication efforts. Direct PV clinical diagnostic samples using nucleic acid (NA) extraction real-time reverse transcriptase polymerase chain reaction (rRT-PCR) instead current standard method virus isolation culture,...
In the Global Polio Laboratory Network (GPLN), poliovirus (PV) screening results from acute flaccid paralysis (AFP) surveillance is based on virus isolation (VI) through cell culture, entailing long turnaround times and amplification of live poliovirus. An alternative Direct Detection strategy (DD-ITD) for viral nucleic acid stools, bypassing need has been developed extensively validated by GPLN partners. A multi-laboratory demonstration project was conceived to field-test DD-ITD method...
Polioviruses (PV), the main causative agent of acute flaccid paralysis (AFP), are positive-sense single-stranded RNA viruses family Picornaviridae. As we approach global polio eradication, accurate and timely detection poliovirus in stool from AFP cases becomes vital to success for these efforts. Direct PV clinical diagnostic samples using nucleic acid (NA) extraction real-time reverse transcriptase polymerase chain reaction (rRT-PCR) instead current standard method virus isolation culture,...