A5035919793- Cytomegalovirus and herpesvirus research
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- Toxoplasma gondii Research Studies
- Angiogenesis and VEGF in Cancer
- Antifungal resistance and susceptibility
- Long-Term Effects of COVID-19
- Herpesvirus Infections and Treatments
- Virus-based gene therapy research
- MicroRNA in disease regulation
- Aquaculture disease management and microbiota
- RNA regulation and disease
- Invertebrate Immune Response Mechanisms
- Viral gastroenteritis research and epidemiology
- Lipid metabolism and disorders
- DNA Repair Mechanisms
- Respiratory viral infections research
- HIV Research and Treatment
- Neuroinflammation and Neurodegeneration Mechanisms
- Atherosclerosis and Cardiovascular Diseases
- Circular RNAs in diseases
- Intensive Care Unit Cognitive Disorders
- Nanoparticles: synthesis and applications
- interferon and immune responses
Christie (Canada)
2014-2025
McGill University Health Centre
2014-2025
McGill University
2014-2025
University of Alabama at Birmingham
1992-2024
Oxford University Hospitals NHS Trust
2020-2024
Andalusian Health Service
2024
Junta de Andalucía
2024
Hospital Universitario Reina Sofía
2024
John Radcliffe Hospital
2023
Universidade de Vigo
2023
<h2>Summary</h2><h3>Background</h3> Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a global pandemic in 2020. Testing is crucial for mitigating public health and economic effects. Serology considered key to population-level surveillance potentially individual-level risk assessment. However, immunoassay performance not been compared on large, identical sample sets. We aimed investigate the of four high-throughput commercial SARS-CoV-2 antibody immunoassays novel...
Treatment of severe COVID-19 is currently limited by clinical heterogeneity and incomplete description specific immune biomarkers. We present here a comprehensive multi-omic blood atlas for patients with varying severity in an integrated comparison influenza sepsis versus healthy volunteers. identify signatures correlates host response. Hallmarks disease involved cells, their inflammatory mediators networks, including progenitor cells myeloid lymphocyte subsets, features the repertoire,...
<ns3:p><ns3:bold>Background:</ns3:bold> The COVID-19 pandemic caused >1 million infections during January-March 2020. There is an urgent need for reliable antibody detection approaches to support diagnosis, vaccine development, safe release of individuals from quarantine, and population lock-down exit strategies. We set out evaluate the performance ELISA lateral flow immunoassay (LFIA) devices.</ns3:p><ns3:p> <ns3:bold>Methods:</ns3:bold> tested plasma COVID (severe acute respiratory...
ABSTRACT Background The COVID-19 pandemic caused >1 million infections during January-March 2020. There is an urgent need for reliable antibody detection approaches to support diagnosis, vaccine development, safe release of individuals from quarantine, and population lock-down exit strategies. We set out evaluate the performance ELISA lateral flow immunoassay (LFIA) devices. Methods tested plasma COVID (SARS-CoV-2) IgM IgG antibodies by using nine different LFIA used a panel samples who...
Sepsis arises from diverse and incompletely understood dysregulated host response processes following infection that leads to life-threatening organ dysfunction. Here we showed neutrophils emergency granulopoiesis drove a maladaptive during sepsis. We generated whole-blood single-cell multiomic atlas (272,993 cells, n = 39 individuals) of the sepsis immune identified populations immunosuppressive mature immature neutrophils. In co-culture, CD66b+ inhibited proliferation activation CD4+ T...
Human adenoviruses typically cause self-limited respiratory, gastrointestinal, and conjunctival infections in healthy children. In late 2021 early 2022, several previously children were identified with acute hepatitis human adenovirus viremia.
During October-November 2021, clinicians at a children's hospital in Alabama identified five pediatric patients with severe hepatitis and adenovirus viremia upon admission. In November clinicians, the Department of Public Health, Jefferson County CDC began an investigation. This activity was reviewed by conducted consistent applicable federal law policy.
The assembly of human cytomegalovirus (HCMV) is thought to be similar that which has been proposed for alphaherpesviruses and involve envelopment tegumented subviral particles at the nuclear membrane followed by export from cell a poorly defined pathway. However, several studies have shown least two tegument virion proteins remain in cytoplasm during HCMV replicative cycle, thereby suggesting cannot acquire its final envelope envelope. We investigated determining intracellular trafficking...
Invasive candidiasis is a major nosocomial infection that difficult to diagnose. Few biochemically defined markers of invasive are known. Initial findings suggested the presence candida enolase in blood may be novel marker for candidiasis.
Journal Article Nosocomial Acquisition of Candida albicans: An Epidemiologic Study Get access Jose A. Vazquez, Vazquez Departments Internal Medicine (Division Infectious Diseases) and Biostatistics, Wayne State University, Detroit, William Beaumont Hospital, Royal Oak, VA Medical Center, Allen Park, Michigan Reprints or correspondence: Dr. Division oflnfectious Diseases, Harper Professional Bldg., 4160 John R., Suite 202, MI 48201. Search for other works by this author on: Oxford Academic...
Abstract Serological detection of antibodies to SARS-CoV-2 is essential for establishing rates seroconversion in populations, and seeking evidence a level antibody that may be protective against COVID-19 disease. Several high-performance commercial tests have been described, but these require centralised laboratory facilities are comparatively expensive, therefore not available universally. Red cell agglutination do special equipment, read by eye, short development times, low cost can...
Although the assembly of herpesviruses has remained an active area investigation, considerable controversy continues to surround cellular location tegument and envelope acquisition. This is particularly evident when proposed pathways for alpha- beta-herpesvirus are compared. We have approached this aspect human cytomegalovirus (HCMV) assembly, specifically, envelopment, by investigating intracellular trafficking viral proteins which localize in cytoplasms infected cells. In study we...
New tools for applying force to animals, tissues, and cells are critically needed in order advance the field of mechanobiology, as few existing enable simultaneous imaging tissue cell deformation well cellular activity live animals. Here, we introduce a novel microfluidic device that enables high-resolution optical deformations while precise mechanical stimuli surface worm's cuticle with pneumatic pressure reservoir. To evaluate performance, compared analytical numerical simulations...
ABSTRACT Candida glabrata has emerged as an important nosocomial pathogen, yet little is known about its epidemiology. We prospectively followed 98 patients admitted to a medical intensive care unit and the bone marrow transplant of university hospital. Samples from environmental surfaces hands hospital personnel were also cultured. Patients with newly acquired C. strains compared controls who culture negative for glabrata. was recovered multiple sites 24 three surfaces. Sixteen (17%) after...
Candida species are important nosocomial pathogens; however, little is known about the epidemiology of lusitaniae, an organism frequently resistant to amphotericin B. We evaluated 98 patients admitted bone marrow transplant and medical intensive care units a tertiary-care hospital. Each patient with C. lusitaniae was matched control patients. Restriction fragment analysis DNA used determine strain relatedness. Seven (7.1%) were identified; five acquired after admission study unit. All...
Phorbol ester tumor promoters activate gene transcription by regulating both the synthesis and posttranslational modification of activator protein 1 (AP-1) factor, c-Jun JunB are components mammalian AP-1 complex. Here we demonstrate that in U-937 human leukemic cells, phorbol esters stimulate phosphorylation amino terminus (JUN) but not (JUNB). Mutational analysis indicates serine-63 -73, which reside within putative regulatory domain JUN, required for constitutive 12-myristate...
In 'a treatment effectiveness trial', a program is evaluated in real-world setting, with an emphasis on achieving high implementation fidelity. Through fidelity assessment, the link between and outcomes systematically ultimately leads to greater understanding of success or failure. This paper reports results study 'Reconnecting Youth' (RY) prevention program. The research questions were (i) was implemented fidelity? (ii) did better predict outcomes? RY indicated drug abuse for school...
Bacterial lipopolysaccharides (LPS) induce innate immune inflammatory responses in endothelial cells by activating toll-like receptor 4 (TLR4) signalling. Here, we investigate the effects of angiopoietin-1 (Ang-1) on LPS-induced TLR4 signalling and role miR-146 family micro RNAs Ang-1 TRL4 signalling.Leucocyte adhesion to human umbilical vein (HUVECs) was detected using fluorescence microscopy. Adhesion molecule, pro-inflammatory cytokine, miR-146a, miR-146b-5p expressions HUVECs were...
Angiopoietin-1 (Ang-1) and its receptor Tie-2 promote vascular integrity angiogenesis. MicroRNAs (miRNAs) are involved in the regulation of many cellular functions, including endothelial cell (EC) survival, proliferation, differentiation. Several reports indicate that these effects miRNAs on EC functions mediated through modulation angiogenesis factor signaling growth (VEGF). To date, very little is known about roles played by promoted Ang-1-Tie-2 axis. Our high-throughput screening...
The human cytomegalovirus (HCMV) IE2 86-kDa protein is a key viral transactivator and an important regulator of HCMV infections. We used the genome cloned as bacterial artificial chromosome (BAC) to construct four mutants with disruptions in regions 86 that are predicted be for its transactivation autoregulatory functions. Three these have mutations remove amino acids 356 359, 427 435, 505 511, which disrupts region implicated activation early promoters, zinc finger domain, putative...
Human cytomegalovirus (HCMV) infection leads to dysregulation of multiple cell cycle-regulatory proteins. In this study, we examined the effects inhibition cyclin-dependent kinase (cdk) activity on viral replication. With drug Roscovitine, a specific inhibitor kinases 1, 2, 5, 7, and 9, have shown that during first 6 h infection, kinase-dependent events occurred included regulated processing accumulation immediate-early (IE) UL122-123 transcripts UL36-37 transcripts. Altered led loss IE1-72...