A5050403187- Angiogenesis and VEGF in Cancer
- Zebrafish Biomedical Research Applications
- Systemic Lupus Erythematosus Research
- Viral Infectious Diseases and Gene Expression in Insects
- Atherosclerosis and Cardiovascular Diseases
- Congenital heart defects research
- Cell Adhesion Molecules Research
- Neurobiology and Insect Physiology Research
- Advanced Battery Materials and Technologies
- Blood Coagulation and Thrombosis Mechanisms
- Conducting polymers and applications
- Vasculitis and related conditions
- Platelet Disorders and Treatments
- Advanced NMR Techniques and Applications
- Oxidative Organic Chemistry Reactions
- Protease and Inhibitor Mechanisms
- Chronic Lymphocytic Leukemia Research
- Phagocytosis and Immune Regulation
- Renal Diseases and Glomerulopathies
- Autoimmune and Inflammatory Disorders Research
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Fuel Cells and Related Materials
- Inflammasome and immune disorders
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Systemic Sclerosis and Related Diseases
Sapporo City General Hospital
2015-2025
Tokyo Women's Medical University
2024
Ehime University
2023
Municipal Hirakata City Hospital
2020
RIKEN Center for Computational Science
2008-2020
University of Massachusetts Chan Medical School
2012-2017
Hokkaido University
2004-2014
Nanzan University
2014
Japan Science and Technology Agency
2002-2013
Health Sciences University of Hokkaido
2010
We demonstrated previously that a single injection of recombinant human macrophage colony-stimulating factor (rhM-CSF) is sufficient for osteoclast recruitment and survival in osteopetrotic (op/op) mice with deficiency osteoclasts resulting from mutation M-CSF gene. In this study, we show vascular endothelial growth (rhVEGF) can similarly induce op/op mice. Osteoclasts predominantly expressed VEGF receptor 1 (VEGFR-1), activity placenta on was comparable to rhVEGF, showing the signal...
The vertebrate olfactory system discriminates a wide variety of odorants by relaying coded information from sensory neurons in the epithelium to cortical areas brain. Recent studies have shown that first step odor discrimination is mediated approximately 1000 distinct receptors, which comprise largest family G-protein-coupled receptors. In present study, we used Ca(2+) imaging and single-cell reverse transcription-PCR techniques identify mouse responding an odorant subsequently clone...
The in vivo roles of the hundreds mammalian G protein–coupled receptors (GPCRs) are incompletely understood. To explore these roles, we generated mice expressing S1 subunit pertussis toxin, a known inhibitor Gi/o signaling, under control ROSA26 locus Cre recombinase–dependent manner (ROSA26PTX). Crossing ROSA26PTX to pancreatic β cells produced offspring with constitutive hyperinsulinemia, increased insulin secretion response glucose, and resistance diet-induced hyperglycemia. This phenotype...
Activated protein C (APC), a natural anticoagulant protease, can trigger cellular responses via protease-activated receptor-1 (PAR1), G protein-coupled receptor for thrombin. Whether this phenomenon contributes to the physiological effects of APC is unknown. Toward answering question, we compared kinetics PAR1 cleavage on endothelial cells by <i>versus</i> did cleave surface, and antibodies inhibited such cleavage. Importantly, however, was ∼10<sup>4</sup>-fold less potent than thrombin in...
Despite decades of studies suggesting that the in vivo adipocyte progenitor resides within vascular niche, exact nature this remains controversial because distinct have attributed adipogenic properties to multiple cell types. Using Cre recombinases labeling lineages, we conduct parallel lineage tracing experiments assess their degree contribution de novo adipogenesis. Although detect occasional adipocytes were traced by endothelial or mural recombinases, these are rare events. On other hand,...
Background —The vascular smooth muscle cell (VSMC) is the central component involved in fibroproliferative response atherogenesis. As lesion advances, VSMCs migrate from media into subendothelial space, thereby forming fibrous plaque lesions. Platelet-derived growth factor (PDGF) has been known to be a potent chemoattractant and mitogen for SMCs, but pathophysiological role of 2 PDGF receptors, receptor-α (PDGFR-α) receptor-β (PDGFR-β) atherogenesis poorly understood. To clarify this...
The coagulation protease Factor Xa (Xa)1 triggers a variety of cellular responses that may be important for inflammatory reactions to tissue injury. Protease-activated receptors (PAR1, PAR2, and PAR4) can mediate signaling in heterologous expression systems. However, other candidate have been described, the extent which one or more PARs account relevant differentiated cells is unknown. We examined endothelial from wild-type PAR-deficient mice. Wild-type responded agonists PAR1, PAR4....
We investigated the cells that express platelet-derived growth factor receptor alpha (PDGFR alpha) during mouse embryogenesis. PDGFR expression has been identified by in situ hybridization or immunohistochemistry using polyclonal antibodies on tissue sectins. Because no immunostaining study whole-mount specimens published to date, we established a new monoclonal antibody (MAb), APA5, for this purpose. Our results differed APA5 stained only paraxial mesoderm, whereas other investigators...
PDGFRα+ mesenchymal progenitor cells are associated with pathological fibro-adipogenic processes. Conversely, a beneficial role for these during homeostasis or in response to revascularization and regeneration stimuli is suggested, but remains be defined. We studied the molecular profile function of order understand mechanisms underlying their fibrosis versus regeneration. show that essential tissue restructuring through injury-stimulated remodeling stromal vascular components,...
The conduction propertes of PVDF polymer gel electrolytes prepared by the phase inversion method were investigated through diffusion coefficient and conductivity in order to confirm mechanism. is associated with total solution uptake which depends on gelation process. introduced stored pores then penetrates into chains for swelling network. In case a highly porous membrane, this process proceeds repeating steps form homogeneous finally. However, low porosity membrane would be stopped if...
Toward identifying the roles of protease-activated receptor-1 (PAR1) and other G protein-coupled receptors important for vascular development, we investigated role Gα 13 in endothelial cells mouse embryo. LacZ inserted into α exon 1 was highly expressed at midgestation. Endothelial-specific knockout embryos died embryonic days 9.5–11.5 resembled PAR1 knockout. Restoration expression by use a Tie2 promoter-driven transgene rescued development endothelial-specific as well day 9.5 phenotype...
Summary Miraculin is a taste‐modifying protein isolated from the red berries of Richadella dulcifica , shrub native to West Africa. by itself not sweet, but it able turn sour taste into sweet taste. This unique property has led increasing interest in this protein. In article, we report high‐yield production miraculin transgenic tomato plants. High and genetically stable expression was confirmed Western blot analysis enzyme‐linked immunosorbent assay. Recombinant accumulated high levels...
When cells die by necrosis in vivo they stimulate an inflammatory response. It is thought that this response triggered when the injured expose proinflammatory molecules, collectively referred to as damage associated molecular patterns (DAMPs), which are recognized or soluble molecules of innate adaptive immune system. Several putative DAMPs and/or their receptors have been identified, but whether and how much participate responses incompletely understood, not previously compared side-by-side...
Abstract Sterile particles cause several chronic, inflammatory diseases, characterized by repeating cycles of particle phagocytosis and cell death. Recent studies have proposed that these processes are driven the NLRP3 inflammasome, a platform activated phagocytosed particles, which controls both caspase-1–dependent death (pyroptosis) mature IL-1β secretion. After phagocytosis, can disrupt lysosomes, inhibitor suggested resulting release lysosomal protease—cathepsin B—into cytosol somehow...