Alicia Sotomayor-González
A5033593900- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- COVID-19 Clinical Research Studies
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- CRISPR and Genetic Engineering
- Vaccine Coverage and Hesitancy
- Respiratory viral infections research
- Kawasaki Disease and Coronary Complications
- Liver Disease and Transplantation
- Hepatitis Viruses Studies and Epidemiology
- Inflammasome and immune disorders
- COVID-19 epidemiological studies
- Spectroscopy Techniques in Biomedical and Chemical Research
- Bacteriophages and microbial interactions
- Bacillus and Francisella bacterial research
- RNA modifications and cancer
- Extracellular vesicles in disease
- Mosquito-borne diseases and control
- Cancer-related molecular mechanisms research
- Virology and Viral Diseases
- vaccines and immunoinformatics approaches
- COVID-19 Impact on Reproduction
- Systemic Lupus Erythematosus Research
University of California, San Francisco
2020-2024
Abbott (United States)
2020-2022
Global Viral
2020-2022
Universidad Católica de Santa Fe
2021
Universidad Nacional Autónoma de México
2016-2019
The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome 2 (SARS-CoV-2) has spread globally, with >365,000 cases in California as of 17 July 2020. We investigated the genomic epidemiology SARS-CoV-2 Northern from late January to mid-March 2020, using samples 36 patients spanning nine counties and Grand Princess cruise ship. Phylogenetic analyses revealed cryptic introduction at least seven different lineages into California, including epidemic WA1 strains...
Abstract We identified a novel SARS-CoV-2 variant by viral whole-genome sequencing of 2,172 nasal/nasopharyngeal swab samples from 44 counties in California. Named B.1.427/B.1.429 to denote its 2 lineages, the emerged around May 2020 and increased 0% >50% sequenced cases September 1, January 29, 2021, exhibiting an 18.6-24% increase transmissibility relative wild-type circulating strains. The carries 3 mutations spike protein, including L452R substitution. Our analyses revealed 2-fold...
Abstract SARS-CoV-2 Delta and Omicron are globally relevant variants of concern. Although individuals infected with at risk developing severe lung disease, infection often causes milder symptoms, especially in vaccinated 1,2 . The question arises whether widespread infections could lead to future cross-variant protection, accelerating the end pandemic. Here we show that without vaccination, induces a limited humoral immune response mice humans. Sera from overexpressing human ACE2 receptor...
Abstract Given the limited availability of serological testing to date, seroprevalence SARS-CoV-2-specific antibodies in different populations has remained unclear. Here, we report very low SARS-CoV-2 two San Francisco Bay Area populations. Seroreactivity was 0.26% 387 hospitalized patients admitted for non-respiratory indications and 0.1% 1,000 blood donors early April 2020. We additionally describe longitudinal dynamics immunoglobulin-G (IgG), immunoglobulin-M (IgM), vitro neutralizing...
ABSTRACT An outbreak of novel betacoronavirus, SARS-CoV-2 (formerly named 2019-nCoV), began in Wuhan, China December 2019 and the COVID-19 disease associated with infection has since spread rapidly to multiple countries. Here we report development DETECTR, a rapid (∼30 min), low-cost, accurate CRISPR-Cas12 based lateral flow assay for detection from respiratory swab RNA extracts. We validated this method using contrived reference samples clinical infected US patients demonstrated comparable...
SARS-CoV-2 infection elicits a distinct host response in nasal swabs and blood that can be used to diagnose COVID-19.
ABSTRACT We report very low SARS-CoV-2 seroprevalence in two San Francisco Bay Area populations. Seropositivity was 0.26% 387 hospitalized patients admitted for non-respiratory indications and 0.1% 1,000 blood donors. additionally describe the longitudinal dynamics of immunoglobulin-G, immunoglobulin-M, vitro neutralizing antibody titers COVID-19 patients. Neutralizing antibodies rise tandem with immunoglobulin levels following symptom onset, exhibiting median time to seroconversion within...
The human gut virome and its early life development are poorly understood. Prior studies have captured single-point assessments with the evolution of infant remaining largely unexplored. We performed viral metagenomic sequencing on stool samples collected longitudinally from a cohort 53 infants age 2 weeks to 3 years (80.7 billion reads), their mothers (9.8 reads) examine compare viromes. asymptomatic consisted bacteriophages, nonhuman dietary/environmental viruses, human-host predominantly...
As novel SARS-CoV-2 variants continue to emerge, it is critical that their potential cause severe disease and evade vaccine-induced immunity rapidly assessed in humans studied animal models. In early January 2021, a variant designated B.1.429 comprising 2 lineages, B.1.427 B.1.429, was originally detected California (CA) shown have enhanced infectivity vitro decreased antibody neutralization by plasma from convalescent patients vaccine recipients. Here we examine the virulence,...
SARS-CoV-2 Delta and Omicron strains are the most globally relevant variants of concern (VOCs). While individuals infected with at risk to develop severe lung disease 1 , infection causes less disease, mostly upper respiratory symptoms 2,3 . The question arises whether rampant spread could lead mass immunization, accelerating end pandemic. Here we show that Delta, but not Omicron, induces broad immunity in mice. sera from Omicron-infected mice only neutralize Delta-infected broadly effective...
Laboratory tests for the accurate and rapid identification of SARS-CoV-2 variants can potentially guide treatment COVID-19 patients inform infection control public health surveillance efforts. Here, we present development validation a variant DETECTR assay incorporating loop-mediated isothermal amplification (LAMP) followed by CRISPR-Cas12 based single nucleotide polymorphism (SNP) mutations in spike (S) gene. This targets L452R, E484K/Q/A, N501Y mutations, at least one which is found nearly...
Interest in porcine epidemic diarrhea has grown since the 2013 outbreak United States caused major losses, with mortality rates up to 100 % suckling piglets. In Mexico, an of diarrhea, characterized by piglets, began March 2014 State Mexico. The aim this study was confirm and identify virus (PEDV) samples from piglets suggestive clinical signs using virological, histological, molecular techniques. Necropsy performed on 13 two litters initial advanced signs. Suggestive lesions acute infection...
Abstract The COVID-19 pandemic caused by the novel coronavirus SARS-CoV-2 has spread globally, resulting in >300,000 reported cases worldwide as of March 21st, 2020. Here we investigate genetic diversity and genomic epidemiology Northern California using samples from returning travelers, cruise ship passengers, community transmission with unclear infection sources. Virus genomes were sampled 29 patients diagnosed Feb 3rd through Mar 15th. Phylogenetic analyses revealed at least 8...
Differential host responses in coronavirus disease 2019 (COVID-19) and multisystem inflammatory syndrome children (MIS-C) remain poorly characterized. Here, we use next-generation sequencing to longitudinally analyze blood samples from pediatric patients with COVID-19 or MIS-C across three hospitals. Profiling of plasma cell-free nucleic acids uncovers distinct signatures cell injury death between MIS-C, increased multiorgan involvement encompassing diverse types, including endothelial...
Summary Virus-like particle (VLP) and live virus assays were used to investigate neutralizing immunity Delta Omicron SARS-CoV-2 variants in 239 samples from 125 fully vaccinated individuals. In uninfected, non-boosted individuals, VLP neutralization titers reduced 2.7-fold 15.4-fold, respectively, compared wild-type (WT), while boosted individuals (n=23) had 18-fold increased titers. breakthrough infections (n=39) 57-fold 3.1-fold whereas (n=14) 5.8-fold 0.32-fold uninfected respectively....
Abstract Background As of early 2022, the Omicron variants are predominant circulating lineages globally. Understanding neutralizing antibody responses against BA.1 and BA.2 after vaccine breakthrough infections will provide insights into infectivity susceptibility to subsequent reinfection. Methods Live virus neutralization assays were used study immunity Delta in samples from 86 individuals, 24 unvaccinated (27.9%) 62 vaccinated (72.1%), who infected with (n = 42, 48.8%) or 44, 51.2%)....
The extent to which vaccinated persons diagnosed with coronavirus disease 2019 (COVID-19) can transmit other and unvaccinated is unclear.
ABSTRACT Inflammatory syndromes, including those caused by infection, are a major cause of hospital admissions among children and often misdiagnosed because lack advanced molecular diagnostic tools. In this study, we explored the utility circulating cell-free RNA (cfRNA) in plasma as an analyte for differential diagnosis characterization pediatric inflammatory syndromes. We profiled cfRNA 370 samples from patients with range conditions, Kawasaki disease (KD), Multisystem Syndrome Children...
Inflammatory syndromes, including those caused by infection, are a major cause of hospital admissions among children and often misdiagnosed because lack advanced molecular diagnostic tools. In this study, we explored the utility circulating cell-free RNA (cfRNA) in plasma as an analyte for differential diagnosis characterization pediatric inflammatory syndromes. We profiled cfRNA 370 samples from patients with range conditions, Kawasaki disease (KD), multisystem syndrome (MIS-C), viral...
Abstract As novel SARS-CoV-2 variants continue to emerge, it is critical that their potential cause severe disease and evade vaccine-induced immunity rapidly assessed in humans studied animal models. In early January 2021, a variant of concern (VOC) designated B.1.429 comprising 2 lineages, B.1.427 B.1.429, was originally detected California (CA) shown enhance infectivity vitro decrease antibody neutralization by plasma from convalescent patients vaccine recipients. Here we examine the...