A5087493610- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Viral Infections and Immunology Research
- Virus-based gene therapy research
- Hepatitis Viruses Studies and Epidemiology
- Respiratory viral infections research
- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- Infection Control and Ventilation
- Milk Quality and Mastitis in Dairy Cows
- Viral Infections and Vectors
- Animal Disease Management and Epidemiology
- Food Allergy and Anaphylaxis Research
- Congenital Anomalies and Fetal Surgery
- Biochemical Analysis and Sensing Techniques
- Advanced Thermodynamic Systems and Engines
- COVID-19 and healthcare impacts
- Microbial Metabolism and Applications
- Biosensors and Analytical Detection
- Coccidia and coccidiosis research
- COVID-19 epidemiological studies
- COVID-19 diagnosis using AI
- Animal Nutrition and Physiology
- Veterinary medicine and infectious diseases
- Parvovirus B19 Infection Studies
Centers for Disease Control and Prevention
2016-2025
National Center for Immunization and Respiratory Diseases
2014-2023
Oak Ridge Institute for Science and Education
2021
University of Wisconsin–Madison
2021
University of Wisconsin–Oshkosh
2021
Chinese University of Hong Kong
2020
University College London
2020
Research Institute for Tropical Medicine
2020
National Institute for Public Health and the Environment
2020
Erasmus MC
2020
Noroviruses are genetically diverse RNA viruses associated with acute gastroenteritis in mammalian hosts. Phylogenetically, they can be segregated into different genogroups as well P (polymerase)-groups and further genotypes P-types based on amino acid diversity of the complete VP1 gene nucleotide RNA-dependent polymerase (RdRp) region ORF1, respectively. In recent years, several new noroviruses have been reported that warrant an update existing classification scheme. Using previously...
Antigen-based tests for SARS-CoV-2, the virus that causes coronavirus disease 2019 (COVID-19), are inexpensive and can return results within 15 minutes (1). Antigen have received Food Drug Administration (FDA) Emergency Use Authorization (EUA) use in asymptomatic symptomatic persons first 5-12 days after symptom onset (2). These been used at U.S. colleges universities other congregate settings (e.g., nursing homes correctional detention facilities), where serial testing of might facilitate...
Noroviruses are the most frequent cause of epidemic acute gastroenteritis in United States. Between September 2013 and August 2016, 2,715 genotyped norovirus outbreaks were submitted to CaliciNet. GII.4 Sydney viruses caused 58% during these years. A virus with a novel GII.P16 polymerase emerged November 2015, causing 60% all 2015-2016 season. Several genotypes detected associated more than one type, including GI.3, GII.2, GII.3, Sydney, GII.13, GII.17, four which harbored polymerases....
Although rotavirus and norovirus cause nearly 40% of severe endemic acute gastroenteritis (AGE) in children <5 years age the United States, there are limited data on etiologic role other enteric viruses this group.We conducted active population-based surveillance presenting with AGE to hospitals, emergency departments, primary care clinics 3 US counties. Stool specimens from these age-matched healthy controls collected between October 2008 September 2009 were tested for adenovirus,...
Abstract Background Real-time reverse transcription polymerase chain reaction (rRT-PCR) and antigen tests are important diagnostics for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Sensitivity of has been shown to be lower than that rRT-PCR; however, data evaluate epidemiologic characteristics affect test performance limited. Methods Paired mid-turbinate nasal swabs were collected from university students staff tested SARS-CoV-2 using both Quidel Sofia SARS Antigen...
We report an increase in GII.17 norovirus outbreaks and sporadic infections of acute gastroenteritis Austria, Germany, France, Ireland, the Netherlands, England United States during 2023/24 season. A decrease GII.4 coincided with prevalence increasing to between 17% 64% all GII detections. Overall, 84% strains clustered closely first reported Romania 2021 two new sub-lineages were identified. Norovirus surveillance molecular characterisation should be prioritised this winter.
The study was conducted to investigate the molecular epidemiology of noroviruses (NoVs) from western India. A total 830 fecal specimens were collected during July 2005-June 2007 children, < or =7 years age suffering acute gastroenteritis in Pune, Nagpur, and Aurangabad cities. All subjected RT-PCR, sequencing phylogenetic analysis for detection characterization Genogroup I (GI) GII NoVs. NoV positivity varied between 6.3% 12.6% different cities with predominance (96.6%). infections very...
Faecal specimens collected from two outbreaks of acute gastroenteritis that occurred in southern Mumbai, India March and October, 2006 were tested for seven different enteric viruses. Among the 218 tested, 95 (43.6%) positive, 73 (76.8%) a single virus 22 (23.2%) multiple Single viral infections both, October showed predominance enterovirus (EV, 33.3% 40%) rotavirus A (RVA, 25%). The other viruses detected these months norovirus (NoV, 12.1% 10%), B (RVB, adenovirus (AdV, 6.1% 7.5%),...
Noroviruses are the major cause of acute gastroenteritis (AGE) in people all ages globally. Standardized genotyping is key for outbreak investigations and surveillance networks.Here we describe validation a one-step conventional RT-PCR assay sequence-based dual typing GI GII noroviruses. This polymerase (P) capsid (C) uses combination previously published oligonucleotide primers amplifying genomic region spanning 3'-end ORF1 5'end ORF2 resulting 579 bp product 570 viruses.The limit detection...
Noroviruses are the leading cause of acute gastroenteritis worldwide with GII.4 Sydney viruses responsible for majority infections until 2023. To study evolutionary dynamics noroviruses in US (2011-2023), we sequenced and analyzed 406 VP1 335 RdRp sequences submitted to CaliciNet. Time-scale analysis showed average rate strains was 5.56 x 10-3 substitutions/site/year emergence a new cluster within every 4 years starting Sydney[P31] from 2011-2015 followed by Sydney[P16] 2016-2020, most...
Human noroviruses are a leading cause of epidemic and sporadic gastroenteritis worldwide. Because most infections either spread directly via the person-to-person route or indirectly through environmental surfaces food, contaminated fomites inanimate important vehicles for virus during norovirus outbreaks. We developed evaluated protocol using macrofoam swabs detection typing human from hard surfaces. Compared with fiber-tipped antistatic wipes, allow recovery (range 1.2-33.6%) toilet seat up...
Abstract Background Norovirus is a leading cause of acute gastroenteritis worldwide, yet there limited information on homotypic or heterotypic protection following natural infection to guide vaccine development. Methods A total 6020 stools collected from 299 Peruvian children between 2010 and 2014 were tested by norovirus real-time reverse-transcription polymerase chain reaction followed sequence-based genotyping. Cox proportional hazards models used derive adjusted hazard ratios (HRs) among...
We report a new norovirus GII.4 variant, Hong Kong, with low-level circulation in 4 Eurasia countries since mid-2017. Amino acid substitutions key residues on the virus capsid associated emergence of pandemic noroviruses suggest that Kong has potential to become next variant.
Abstract We detected a novel GII.4 variant with an amino acid insertion at the start of epitope A in viral protein 1 noroviruses from United States, Gabon, South Africa, and Kingdom collected during 2017–2022. Early identification variants is crucial for assessing pandemic potential informing vaccine development.
This study pertains to the characterization of a human rotavirus strain (NIV929893) with rare specificity G1P[19]. Three structural genes (VP4, VP6 and VP7) one non-structural gene (NSP4) NIV929893 were subjected RT-PCR for amplification entire coding regions. All amplicons sequenced carry out phylogenetic analysis. The complete amino acid sequences VP7 VP4 products showed clustering G1 strains origin P[19] porcine origin. two viral proteins NSP4, described previously as genetically linked...
Abstract After the introduction of rotavirus vaccine into Universal Immunization Program in India 2016, relatively few studies have assessed prevalence and epidemiological patterns acute gastroenteritis (AGE) among hospitalized children ≤5 years age. We used a uniform protocol to recruit with AGE as well standardized testing typing protocols. Stool specimens from younger than 5 age admitted six hospitals three cities were collected January 2017 through December 2019. Norovirus was detected...