A5090797096- Viral gastroenteritis research and epidemiology
- Viral Infections and Immunology Research
- Virus-based gene therapy research
- Animal Virus Infections Studies
- Respiratory viral infections research
- Hepatitis Viruses Studies and Epidemiology
- Child Nutrition and Water Access
- Salmonella and Campylobacter epidemiology
- Microbial Inactivation Methods
- Listeria monocytogenes in Food Safety
- Animal Disease Management and Epidemiology
- Infant Nutrition and Health
- Bacteriophages and microbial interactions
- Antimicrobial Resistance in Staphylococcus
- Advanced Thermodynamic Systems and Engines
- Veterinary medicine and infectious diseases
- Milk Quality and Mastitis in Dairy Cows
- Food Safety and Hygiene
- Parvovirus B19 Infection Studies
- Probiotics and Fermented Foods
- Bacterial Identification and Susceptibility Testing
- Plant Virus Research Studies
- Identification and Quantification in Food
- Infection Control and Ventilation
- Bacterial biofilms and quorum sensing
Centers for Disease Control and Prevention
2016-2024
The Centers
2024
Emory University
2020-2022
National Center for Immunization and Respiratory Diseases
2017-2022
Oak Ridge Associated Universities
2018-2020
Oak Ridge Institute for Science and Education
2018-2020
Universidad de Burgos
2013-2015
Instituto Tecnológico Agrario de Castilla y León
2010-2015
Junta de Castilla y León
2010-2015
Veterinary Research Institute
2012
We evaluated the prevalence of hepatitis E virus (HEV) in pork production chain Czech Republic, Italy, and Spain during 2010. A total 337 fecal, liver, meat samples from animals at slaughterhouses were tested for HEV by real-time quantitative PCR. Overall, was higher Italy (53%) (39%) than Republic (7.5%). detected most frequently feces (41%) liver (5%) (2.5%) Republic. Of 313 sausages sampled processing point sale, only (6%). sequencing confirmed g3 strains. Indicator (porcine adenovirus)...
Hepatitis E virus (HEV) genotype 3 and 4 can cause liver disease in human has its main reservoir pigs. HEV investigations pigs worldwide have been performed but there is still a lack of information on the infection dynamics pig populations.The transmission commercial farms six different European countries was studied. The data collected show prevalence weaners ranging from 8% to 30%. average growers between 20% 44%. fatteners ranged 73%. Sows similar all countries. Boar faeces were tested...
Noroviruses are the major cause of acute gastroenteritis (AGE) in people all ages globally. Standardized genotyping is key for outbreak investigations and surveillance networks.Here we describe validation a one-step conventional RT-PCR assay sequence-based dual typing GI GII noroviruses. This polymerase (P) capsid (C) uses combination previously published oligonucleotide primers amplifying genomic region spanning 3'-end ORF1 5'end ORF2 resulting 579 bp product 570 viruses.The limit detection...
Internal amplification controls (IACs) were constructed for incorporation into real-time nucleic acid assays bovine polyomavirus, hepatitis A virus, E human adenovirus, norovirus genogroup I, II, murine and porcine adenovirus. The addition of optimised amounts IAC the did not affect limits detection each specific target virus. poorly performed extraction viral acids was simulated, effectiveness IACs in identifying failed demonstrated. this study can be reliably used their to provide a robust...
Synthetic multiple-target RNA and DNA oligonucleotides were constructed for use as quantification standards nucleic acid amplification assays human norovirus genogroup I II, hepatitis E virus, murine norovirus, adenovirus, porcine adenovirus bovine polyomavirus. This approach overcomes the problems related to difficulty of obtaining practical quantities viral from these viruses. The capacity using was excellent in each case (R(2) > 0.998 PCR efficiency 0.89). copy numbers equivalent genome...
The role of histo-blood group on the burden and severity norovirus gastroenteritis in young infants has not been well documented.Norovirus was assessed 443 Nicaraguan children followed from birth until 3 years age. Stool samples were tested for by reverse-transcription quantitative polymerase chain reaction (RT-qPCR), antigens (HBGAs) determined phenotyping saliva blood. Hazard ratios predictors acute (AGE) outcome stratified HBGA estimated using Cox proportional hazards models.Of 1353 AGE...
Sample process controls (SPCs) are an essential component of methods to detect viruses in food, as they verify that the sample treatment has operated correctly. Also, use SPC can allow efficiency extraction target be estimated for each individual analysed. The murine norovirus is here described. Its from different food products was 39.47%, 24.79% and 36.29% strawberry, lettuce shellfish samples. An incorrectly performed modelled demonstrate effectiveness this control.
Sapovirus is increasingly recognized as an important cause of acute gastroenteritis (AGE) in children. We identified risk factors and characterized the clinical profile sapovirus AGE a birth cohort León, Nicaragua.We conducted case-control study nested within (n = 444). Fieldworkers weekly household surveillance. stools were tested for by reverse transcriptase quantitative polymerase chain reaction. For each first episode, we selected 2 healthy age-matched controls estimated independent...