A5049085262- Viral gastroenteritis research and epidemiology
- Viral Infections and Immunology Research
- Hepatitis Viruses Studies and Epidemiology
- Animal Virus Infections Studies
- Hepatitis B Virus Studies
- Liver Diseases and Immunity
- Virus-based gene therapy research
- Liver Disease and Transplantation
- Congenital Anomalies and Fetal Surgery
- Child Nutrition and Water Access
- Hepatitis C virus research
- Systemic Lupus Erythematosus Research
- Salmonella and Campylobacter epidemiology
- Peripheral Neuropathies and Disorders
- Animal Disease Management and Epidemiology
- Clostridium difficile and Clostridium perfringens research
- Infective Endocarditis Diagnosis and Management
- Cardiac Structural Anomalies and Repair
- Parvovirus B19 Infection Studies
- Animal health and immunology
- Pericarditis and Cardiac Tamponade
- Hemoglobinopathies and Related Disorders
- Advanced Thermodynamic Systems and Engines
- Liver Disease Diagnosis and Treatment
- Mycobacterium research and diagnosis
ICMR-National Institute of Virology
2011-2020
Maharashtra Animal and Fishery Sciences University
2018
Baba Raghav Das Medical College
2009-2018
National Institute of Immunology
1988
Current, nationally representative data on rotavirus disease burden and strains in India are needed to understand the potential health benefits of vaccination.The Indian Rotavirus Strain Surveillance Network was established with 4 laboratories 10 hospitals 7 different regions India. At each hospital, children aged <5 years who presented acute gastroenteritis required hospitalization rehydration for at least 6 h were enrolled. A fecal specimen obtained tested use a commercial enzyme...
Hand, foot and mouth disease (HFMD), a common contagious that usually affects children, can be caused by enteroviruses. Coxsackievirus A16 (CV-A16) enterovirus 71(EV-71) are the major aetiological agents of HFMD. Other EV serotypes, CV-A4-7, CV-A9-10, CV-B1-3, CV-B5, E-4 E-19, have also been found associated with both sporadic infections outbreaks In India, HFMD documented; however, molecular characterization has rarely reported. Cases were identified during 2009-2010 on basis clinical...
Abstract An outbreak of viral encephalitis occurred in northern India 2006. Attempts to identify an etiologic agent cerebrospinal fluid by using reverse transcription–PCR showed positivity enterovirus (EV) 66 (21.6%) 306 patients. Sequencing and phylogenetic analyses PCR products from 59 (89.3%) specimens similarity with EV-89 EV-76 sequences.
Non-polio enteroviruses (NPEVs) have been reported frequently in association with acute flaccid paralysis (AFP) cases during Polio Surveillance Programs (PSPs) worldwide. However, there is limited understanding on the attributes of their infections. This study reports characteristics NPEVs isolated from AFP cases, investigated PSPs held 2009–2010, Karnataka and Kerala states south-western India having varied climatic conditions. NPEV cell culture isolates derived stool specimens that were...
The epidemiology of hepatitis A virus (HAV) and E (HEV) was assessed among age‐stratified urban high socioeconomic, lower middle socioeconomic status rural populations from western India in 1998. When compared with previous surveys, a clear shift to intermediate endemicity HAV evident only for higher population (1982–98), raising the possibility outbreaks this category. decrease anti‐HAV positivity noted children aged 6–10 years. Lower circulation HEV < 25‐year‐old individuals. For both...
The study was conducted to investigate the molecular epidemiology of noroviruses (NoVs) from western India. A total 830 fecal specimens were collected during July 2005-June 2007 children, < or =7 years age suffering acute gastroenteritis in Pune, Nagpur, and Aurangabad cities. All subjected RT-PCR, sequencing phylogenetic analysis for detection characterization Genogroup I (GI) GII NoVs. NoV positivity varied between 6.3% 12.6% different cities with predominance (96.6%). infections very...
Faecal specimens collected from two outbreaks of acute gastroenteritis that occurred in southern Mumbai, India March and October, 2006 were tested for seven different enteric viruses. Among the 218 tested, 95 (43.6%) positive, 73 (76.8%) a single virus 22 (23.2%) multiple Single viral infections both, October showed predominance enterovirus (EV, 33.3% 40%) rotavirus A (RVA, 25%). The other viruses detected these months norovirus (NoV, 12.1% 10%), B (RVB, adenovirus (AdV, 6.1% 7.5%),...
Acute gastroenteritis is a major cause of childhood morbidity and mortality worldwide. Rotavirus (RV) Norovirus (NoV) are the leading disease. Despite use improved diagnostic methods significant proportion cases remained undiagnosed. Though nonpolio enteroviruses (NPEVs) have been reported frequently in children with acute gastroenteritis, their etiologic role has not established. To investigate epidemiology NPEVs which negative for causative agents, 955 RV NoV stool specimens from...
Abstract Enteric adenoviruses are important etiological agents associated with sporadic infections and outbreaks of acute gastroenteritis in infants children. Fecal samples were collected from 439 hospitalized patients the years 2005–2007 Pune, Aurangabad, Nagpur cities western India to identify most prevalent strains enteric adenoviruses. The viruses detected by PCR characterized sequencing phylogenetic analysis. prevalence Aurangabad was found be 9% (10/111), 7% (7/100), 7.5% (17/228),...
A vast diversity in rotaviruses at inter- and intra-genotypic level underscores the need for monitoring of circulating rotavirus strains. The aim this study was to update data on disease strains period from January 2009 December 2012 Pune, western India which has been one sites Indian Rotavirus Strain Surveillance Network since November 2005.Children aged <5 years admitted acute gastroenteritis three different hospitals Pune city were included study. stool specimens collected tested antigen...
A total of 1,591 fecal specimens were collected in 1993-1996 and 2004-2007 from adolescents adults with acute gastroenteritis Pune, India for detection characterization rotavirus. At the two time points, group rotavirus was detected 8.6% 16.2% 5.2% 17.2% adults, respectively. Reverse transcription-PCR consensus primers followed by multiplex genotyping PCR common strains G1P[8], G2P[4], G3P[8], G4P[8] a 53.1% samples 1993 to 1996, while only prevalent strain identified G2P[4] (23.5% total)....
The majority of human group A rotaviruses possess the P[8] VP4 genotype. Recently, a genetically distinct subtype genotype, also known as OP354-like or lineage P[8]-4, emerged in several countries. However, it is unclear for how long gene has been circulating humans and spread. In global collaborative effort 98 (near-)complete sequences were obtained used phylogeographic analysis to determine viral migration patterns. During sampling period, 1988–2012, we found that South East Asia acted...
Rotavirus is an important cause of severe diarrhea requiring hospitalization, accounting for approximately 78,000 deaths annually in Indian children below 5 years age. We present epidemiological data on rotavirus disease collected during hospital-based surveillance India before the introduction oral vaccine into national immunization schedule.The National Surveillance Network was created involving 28 hospital sites and 11 laboratories across four geographical regions India. From September...
Abstract Recently, rotavirus antigenemia and viremia have been identified in patients with acute gastroenteritis. This study examined children hospitalized for gastroenteritis order to establish its association fecal shedding of rotavirus, infecting genotypes antibody marker infection. Thirty‐one pairs stool–serum specimens were collected from November 2004 February 2005 together clinical information. All paired screened RNA by RT‐PCR using the VP6 gene primers. stool serum tested antigen...
This study pertains to the characterization of a human rotavirus strain (NIV929893) with rare specificity G1P[19]. Three structural genes (VP4, VP6 and VP7) one non-structural gene (NSP4) NIV929893 were subjected RT-PCR for amplification entire coding regions. All amplicons sequenced carry out phylogenetic analysis. The complete amino acid sequences VP7 VP4 products showed clustering G1 strains origin P[19] porcine origin. two viral proteins NSP4, described previously as genetically linked...
The G1P[8] rotaviruses are a common cause of rotavirus diarrhoea among children in India. Two vaccines licensed India, Rotarix and RotaTeq, contain strains with G1 P[8] genotypes. A comparative analysis these genotypes the live circulating is essential for assessment diversity.G1P[8] detected during surveillance diarrhoeic hospitalized Pune 1992-1993 2006-2008, were included study. Amplification, sequencing phylogenetic VP7 VP4 genes carried out identification lineages, respectively....
Abstract Background From 2016, the Government of India introduced oral rotavirus vaccine into national immunization schedule. Currently, two indigenously developed vaccines (ROTAVAC, Bharat Biotech; ROTASIIL, Serum Institute India) are included in Indian program. We report disease burden and diversity genotypes from 2005 to 2016 a multi-centric surveillance study before introduction vaccines. Methods A total 29,561 stool samples collected (7 sites during 2005–2009, 3 2009 2012, 28 2012–2016)...