Human hair proteins were isolated and purified for the fabrication of tissue-engineering scaffolds. Their cellular compatibility was studied using NIH3T3 mice fibroblast cells. The characterized FTIR spectroscopy, sodium dodecyl sulfate-polyacrylamide gel electrophoresis molecular weights two-dimensional polyacrylamide their isoelectric points (pIs). keratins in range 40-60 kilo-Daltons (kDa) matrix 15-30 kDa. pIs found to be 4.5-5.3. Sponges formed by lyophilization. Scanning electron...

Live oral rotavirus vaccines have been less immunogenic and efficacious among children in poor developing countries compared with middle income industrialized for reasons that are not yet completely understood. We assessed whether the neutralizing activity of breast milk could lower titer vaccine virus explain this difference vitro.Breast samples were collected from mothers who breast-feeding infants 4 to 29 weeks age (ie, eligible age) India (N = 40), Vietnam 77), South Korea 34), United...

A total of 62,475 children <5 years old from a defined population approximately 500,000 and adults slums in New Delhi, India, were assessed for 1 year by means passive surveillance, to identify who hospitalized diarrhea. The incidence severe rotavirus diarrhea was estimated, the G P types infecting strains determined correlated with clinical severity Of 584 diarrhea, 137 (23.5%) had detected stool specimens (incidence diarrhea-associated hospitalizations, 337 hospitalizations/100,000 age)....

The prospect that rotavirus diarrhea in children may soon be prevented by vaccines has placed a new priority on understanding the diversity of strains and mechanism which these evolve over time. We have characterized total 465 collected North India from 2000 to 2007 for G P types reverse transcription-PCR sequencing. novel G12 recently detected other countries were first 2001 emerged as predominant Delhi, India, during 2005 2007. While VP7 sequence was highly homologous among isolated...

Background Chikungunya (CHIKV) has recently seen a re-emergence in India with high morbidity. However, the epidemiology and disease burden remain largely undetermined. A prospective multi-centric study was conducted to evaluate clinical, epidemiological virological features of chikugunya infection patients acute febrile illness from various geographical regions India. Methods Findings total 540 fever up 7days duration were enrolled at Karnataka Institute Medical Sciences (KIMS), (South);...

Live oral rotavirus vaccines have been less immunogenic and efficacious for children of developing countries than those in middle income industrialized countries, the basis these differences is not fully understood. Recently, we demonstrated that breastmilk from mothers India had significantly higher IgA neutralizing activity against could reduce effective titer reaching gut when compared with United States. We extended our study to understand specific contribution nonantibody components...

Rotaviral antigen and RNA have recently been identified in the serum of patients with rotaviral gastroenteritis, but roles they play disease remains undetermined.Rotaviral were quantified by enzyme-linked immunosorbant assay quantitative reverse-transcription polymerase chain reaction stool specimens from children diarrhea (n=102), nonrotaviral (n=40), nondiarrheal control (n=30).Rotaviral antigenemia was detected 64%, 3%, 0% diarrhea, children, respectively. The level approximately 2x10(2)...

Cashew apple is considered as a waste in cashew nut processing industry. An undistilled alcoholic beverage (wine) was prepared by fermenting juice with wine yeast, Saccharomyces cerevisiae var. bayanus. The slightly yellowish, acidic taste (titratable acidity [1.21 ± 0.0 g tartaric acid/100 mL]), high tannin (1.9 0.22 mg/100 mL) and low alcohol (7%) concentration. Although sensory evaluation rated the quite acceptable an beverage, significant differences (P < 0.01) exist between commercial...

There are very few studies that have assessed multiple viral agents causing Acute‐Gastroenteritis (AGE) in India. The present study compared the changing pattern of prevalence and genetic diversity five enteric viruses associated with acute‐diarrhea Delhi children within a gap 5 years. Fecal samples were collected from diarrheal (&lt;4 years) during two winter seasons: year 2009‐2010 ( n = 59) 2014‐2015 85). Samples individually tested for rotavirus‐A, norovirus, astrovirus, adenovirus,...

BackgroundRotaviruses cause acute viral gastroenteritis worldwide. It has been estimated that, each year, 440,000 deaths that occur among children are attributed to rotavirus infection, mainly in developing countries. In India, the diversity of rotaviruses reported during 1980s and 1990s emphasizes need for surveillance cocirculating strains, follow rapid changes circulation detect novel strains.

Polylactide (PLA) and polylactide-co-glycolide (PLGA) particles entrapping rotavirus (strain SA11) were formulated using a solvent evaporation technique. To minimize denaturation of viral antigen during the emulsification process, serum albumin was used as stabilizer. Use NaHCO3 sucrose primary step resulted in uniform stabilized rotavirus. Sonication emulsion homogenization secondary process sizes 2–8 μm, whereas nanoparticles formed when sonication both processes. Scanning electron atomic...

Rotavirus genotype G12 strains were detected for the first time among newborns with asymptomatic rotavirus infection (74% of 39 isolated from infected infants G12) in nursery All India Institute Medical Sciences during a period 2005 to 2006. Sequence analysis VP7 genes these neonatal indicated high level homology other reported worldwide, suggesting recent emergence humans. Such nosocomial infections represent potential source introduction novel serotypes into community.

Since its discovery, Chikungunya fever caused by a virus (CHIKV) has ravaged most of Africa and Southeast Asia. Despite there being more than million reported cases in India alone the seriousness disease chronic phase, clear understanding pathogenesis host response remains elusive. Here, we use microarray technology quantitative PCR method to establish complete miRNA, snoRNA mRNA signature upon CHIKV infection human cell line model, HEK293T. The results were further validated primary cells...

Chikungunya virus (CHIKV) infection is a long known mosquito-borne disease that associated with severe morbidity, characterized by fever, headache, rashes, joint pain, and myalgia. It believed load has relation severity of clinical features.We performed this study to assess the relationship between features in children during acute phase CHIKV infection, order draw insights for better-informed treatment.Between June 1, 2009, May 31, 2010, 338 patients fever susceptive first 4 days illness...

Abstract Scaffolds of agar and gelatin were developed using a novel entrapment method where molecules mutually entrapped one another forming stable cell adhesive matrices. Glutaraldehyde was used as crosslinking agent for gelatin. Three types hybrid matrices prepared in different proportions the weight ratio 1:1, 2:1, 3:1. Surface characterization dry scaffolds carried out by scanning electron microscope. Swelling studies phosphate buffer saline (PBS) at physiological pH 7.4. The integral...

In the present investigation we molecularly characterized nontypeable rotavirus strains previously identified during surveillance in New Delhi, India. The majority of were demonstrated to belong genotype G1 (54.5%) or P[8] (77.8%) on basis nucleotide sequencing fragments from their VP7 and VP4 genes. other genotypes detected included G2, G8, G9, G12, P[4]. A G8P[6] strain, strain DS108, was for first time northern gene DS108 most homologous with a bovine G8 A5 (98.9%), indicating its...

Rotavirus is the leading cause of severe gastroenteritis in young children worldwide and responsible for around 100,000 deaths India annually. Vaccination against rotavirus (RV) a high priority: 'ROTAVAC' an indigenous vaccine will soon be licensed India. Surveillance to determine impact vaccines on emerging RV strains required. In this study we compared pattern circulating Delhi over 5 year period with past 12 years. The most commonly detected G genotypes were G1 (22.4%), G2 (17.2%), G9...

Abstract There are limited reports on the etiology of multiple enteric viruses causing acute gastroenteritis (AGE) in North India. In present study we have determined prevalence three viruses, namely rotavirus, astrovirus (AstV) and adenovirus (AdV) a total 312 diarrheic children (&lt;5 years) hospitalized at Lala Lajpat Rai Memorial Medical College, Meerut, Uttar Pradesh from August 2014 to July 2016; results were compared with data Delhi. The fecal samples individually screened for group A...