A5060030666- Viral gastroenteritis research and epidemiology
- Respiratory viral infections research
- Influenza Virus Research Studies
- SARS-CoV-2 detection and testing
- Virus-based gene therapy research
- Animal Virus Infections Studies
- Viral Infections and Immunology Research
- SARS-CoV-2 and COVID-19 Research
- Animal Disease Management and Epidemiology
- Biosensors and Analytical Detection
- Viral Infections and Vectors
- Bacteriophages and microbial interactions
- Immune Cell Function and Interaction
- Parvovirus B19 Infection Studies
- Advanced biosensing and bioanalysis techniques
- COVID-19 Clinical Research Studies
- Immune Response and Inflammation
- Microbial Community Ecology and Physiology
- Viral Infections and Outbreaks Research
- interferon and immune responses
- Advanced Thermodynamic Systems and Engines
- Infection Control and Ventilation
- Gut microbiota and health
- COVID-19 epidemiological studies
- Plant Virus Research Studies
Erasmus MC
2014-2024
Erasmus University Rotterdam
2012-2024
KWR Water Research Institute
2021
University of Edinburgh
2013-2021
Technical University of Denmark
2021
Chinese University of Hong Kong
2020
University College London
2020
Research Institute for Tropical Medicine
2020
Centers for Disease Control and Prevention
2020
National Institute for Public Health and the Environment
2016-2020
Generation of Swine Flu As the newly emerged influenza virus starts its journey to infect world's human population, genetic secrets 2009 outbreak swine A(H1N1) are being revealed. In extensive phylogenetic analyses, Garten et al. (p. 197 , published online 22 May) confirm that eight elements virus, basic components encoded by hemagglutinin, nucleoprotein, and nonstructural genes originated in birds transferred pigs 1918. Subsequently, these formed a triple reassortant with RNA polymerase PB1...
Intestinal organoids as an infection model Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes influenza-like disease with a transmission route; however, patients often present gastrointestinal symptoms such diarrhea, vomiting, and abdominal pain. Moreover, the virus has been detected in anal swabs, cells inner-gut lining express receptor that SARS-CoV-2 uses to gain entry cells. Lamers et al. used human intestinal organoids, “mini-gut” cultured dish, demonstrate readily...
ABSTRACT A novel human coronavirus (HCoV-EMC/2012) was isolated from a man with acute pneumonia and renal failure in June 2012. This report describes the complete genome sequence, organization, expression strategy of HCoV-EMC/2012 its relation known coronaviruses. The contains 30,119 nucleotides at least 10 predicted open reading frames, 9 which are to be expressed nested set seven subgenomic mRNAs. Phylogenetic analysis replicase gene coronaviruses completely sequenced genomes showed that...
Noroviruses are genetically diverse RNA viruses associated with acute gastroenteritis in mammalian hosts. Phylogenetically, they can be segregated into different genogroups as well P (polymerase)-groups and further genotypes P-types based on amino acid diversity of the complete VP1 gene nucleotide RNA-dependent polymerase (RdRp) region ORF1, respectively. In recent years, several new noroviruses have been reported that warrant an update existing classification scheme. Using previously...
In the winter of 2014/15 a novel GII.P17-GII.17 norovirus strain (GII.17 Kawasaki 2014) emerged, as major cause gastroenteritis outbreaks in China and Japan. Since their emergence these viruses have replaced previously dominant GII.4 genotype Sydney 2012 variant some areas Asia but were only detected limited number cases on other continents. This perspective provides an overview available information GII.17 order to gain insight viral host characteristics this genotype. We further discuss...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has rapidly become a major global health problem, and public surveillance is crucial to monitor prevent virus spread. Wastewater-based epidemiology been proposed as an addition disease-based because shed in the feces of ≈40% infected persons. We used next-generation sequencing sewage samples evaluate diversity SARS-CoV-2 at community level Netherlands Belgium. Phylogenetic analysis revealed presence most prevalent clades (19A, 20A,...
Abstract COVID-19, caused by SARS-CoV-2, is an influenza-like disease with a respiratory route of transmission, yet clinical evidence suggests that the intestine may present another viral target organ. Indeed, SARS-CoV-2 receptor angiotensin converting enzyme 2 (ACE2) highly expressed on differentiated enterocytes. In human small intestinal organoids, enterocytes were readily infected SARS-CoV and as demonstrated confocal- electron-microscopy. Consequently, significant titers infectious...
Wastewater surveillance has shown to be a valuable and efficient tool obtain information about the trends of COVID-19 in community. Since recent emergence new variants, associated with increased transmissibility and/or antibody escape (variants concern), there is an urgent need for methods that enable specific timely detection quantification occurrence these variants In this study, we demonstrate use RT-ddPCR on wastewater samples mutation N501Y. This assay enabled simultaneous enumeration...
ABSTRACT The discovery of human metapneumovirus and its implications for respiratory tract disease have emphasized the need a sensitive, specific, rapid assay to detect this virus in clinical setting. It recently became clear that can be grouped into at least four genetic lineages. Previously described assays detection were developed by using limited sequence information failed viruses from all lineages with comparable sensitivities. Here we describe development evaluation real-time reverse...
ABSTRACT Influenza A(H3N2) viruses became widespread in humans during the 1968 H3N2 virus pandemic and have been a major cause of influenza epidemics ever since. These evolve continuously by reassortment genomic evolution. Antigenic drift is for need to update vaccines frequently. Using two data sets that span entire period circulation human viruses, it was shown evolution can be mapped 13 antigenic clusters. Here we analyzed full genomes 286 from these investigate patterns. Numerous events...
Over the course of Corona Virus Disease-19 (COVID-19) pandemic in 2020–2022, monitoring severe acute respiratory syndrome coronavirus 2 ribonucleic acid (SARS-CoV-2 RNA) wastewater has rapidly evolved into a supplementary surveillance instrument for public health. Short term trends (2 weeks) are used as basis policy and decision making on measures dealing with pandemic. Normalisation is required to account dilution rate domestic that can strongly vary due time- location-dependent sewer...
Human (HMPV) and avian (AMPV) metapneumoviruses are closely related viruses that cause respiratory tract illnesses in humans birds, respectively. Although HMPV was first discovered 2001, retrospective studies have shown has been circulating for at least 50 years. AMPV isolated the 1970s, can be classified into four subgroups, A-D. subgroup C is more to than any other subgroup, suggesting emerged from AMPV-C upon zoonosis. Presently, genetic lineages of circulate human populations - A1, A2,...
Each year, influenza viruses cause epidemics by evading pre-existing humoral immunity through mutations in the major glycoproteins: haemagglutinin (HA) and neuraminidase (NA). In 2004, antigenic evolution of HA human A (H3N2) was mapped (Smith et al. , Science 305 371–376, 2004) from its introduction humans 1968 until 2003. The current study focused on genetic NA compared it with using dataset Smith colleagues, updated to epidemic 2009/2010 season. Phylogenetic trees maps were constructed...
ABSTRACT The majority of currently circulating influenza A(H1N1) viruses are antigenically similar to the virus that caused 2009 pandemic. However, antigenic variants expected emerge as population immunity increases. Amino acid substitutions in hemagglutinin protein can result escape from neutralizing antibodies, affect viral fitness, and change receptor preference. In this study, we constructed mutants with A/Netherlands/602/09 an attenuated backbone explore amino changes may contribute...
Analysis of complete capsid sequences the emerging norovirus GII.17 Kawasaki 308 from 13 countries demonstrated that they originated a single haplotype since initial emergence in China late 2014. Global spread sublineage SL2 was identified. A new SL3 emerged 2016.
During viral infections cellular gene expression is subject to rapid alterations induced by both and antiviral mechanisms. In this study, we applied metabolic labeling of newly transcribed RNA with 4-thiouridine (4sU-tagging) dissect the real-time kinetics transcriptional activity during lytic murine cytomegalovirus (MCMV) infection. Microarray profiling on obtained at different times first six hours MCMV infection revealed discrete functional clusters genes regulated distinct surprising...