A5012816836- Viral gastroenteritis research and epidemiology
- Viral Infections and Immunology Research
- Animal Virus Infections Studies
- Virus-based gene therapy research
- Fecal contamination and water quality
- Hepatitis Viruses Studies and Epidemiology
- SARS-CoV-2 detection and testing
- Respiratory viral infections research
- Child Nutrition and Water Access
- SARS-CoV-2 and COVID-19 Research
- Salmonella and Campylobacter epidemiology
- Biosensors and Analytical Detection
- Multiple Myeloma Research and Treatments
- Cancer Treatment and Pharmacology
- Enzyme function and inhibition
- Chemical Synthesis and Analysis
- Bacteriophages and microbial interactions
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Cancer therapeutics and mechanisms
- Lung Cancer Research Studies
- COVID-19 diagnosis using AI
- Neuroendocrine Tumor Research Advances
- COVID-19 epidemiological studies
- Protein Degradation and Inhibitors
- Cancer survivorship and care
Institute of Environmental Science and Research
2015-2025
Canadian Council on International Law
2017-2019
University of Alberta
2004-2016
Centers for Disease Control and Prevention
2015
Erasmus MC
2015
National Institute for Public Health and the Environment
2015
Bournemouth University
2015
University of Auckland
2011-2013
Manchester Royal Infirmary
2011
Cancer Institute (WIA)
1998-2002
Noroviruses (NoVs) are the most common cause of viral gastroenteritis. Their high incidence and importance in health care facilities result a great impact on public health. Studies from around world describing increasing prevalence have been difficult to compare because differing nomenclatures for variants dominant genotype, GII.4. We studied global patterns GII.4 epidemiology relation its genetic diversity.Data NoV outbreaks with dates onset January 2001 through March 2007 were collected 15...
Globally, surveillance systems showed an increase in norovirus activity late 2012. Molecular data shared through the NoroNet network suggest that this is related to emergence of a new genotype II.4 variant, termed Sydney Healthcare institutions are advised be prepared for severe season.
In the winter of 2014/15 a novel GII.P17-GII.17 norovirus strain (GII.17 Kawasaki 2014) emerged, as major cause gastroenteritis outbreaks in China and Japan. Since their emergence these viruses have replaced previously dominant GII.4 genotype Sydney 2012 variant some areas Asia but were only detected limited number cases on other continents. This perspective provides an overview available information GII.17 order to gain insight viral host characteristics this genotype. We further discuss...
Abstract Noroviruses are a leading cause of acute gastroenteritis (AGE) among adults and children worldwide. NoroSurv is global network for norovirus strain surveillance <5 years age with AGE. Participants in 16 countries across 6 continents used standardized protocols dual typing (genotype polymerase type) uploaded 1,325 dual-typed sequences to the web portal during 2016–2020. More than 50% submitted were GII.4 Sydney[P16] or Sydney[P31] strains. Other common strains included GII.2[P16],...
Human and animal fecal pollution of the environment presents a risk to human health because presence pathogenic viruses bacteria. To distinguish between sources pollution, we designed specific real-time reverse transcription (RT)-PCR assays for enteric viruses, including norovirus genogroups I, II, III; porcine adenovirus types 3 5; ovine adenovirus; atadenovirus; species C F, which are excreted by infected humans, pigs, cattle, sheep, deer, goats, detection F+ RNA bacteriophage I IV,...
Background.Acute gastroenteritis is commonly associated with norovirus genogroup II (GII) infection. Norovirus GII has 17 classified genotypes (GII.1–GII.17), but only 1 genotype (GII.4) global epidemics of gastroenteritis. In 2006, an increase in activity was observed. Methods.During the period from December 2005 through August a total 231 fecal samples were obtained patients acute Australia and New Zealand. RNA amplified sequenced to determine relatedness known epidemic GII.4 variants....
Abstract Worldwide, noroviruses are a leading cause of gastroenteritis. They can be transmitted from person to directly or indirectly through contaminated food, water, environments. To estimate the proportion foodborne infections caused by on global scale, we used norovirus transmission and genotyping information multiple international outbreak surveillance systems (Noronet, CaliciNet, EpiSurv) systematic review peer-reviewed literature. The outbreaks food was determined genotype and/or...
Aims: To determine the suitability of murine norovirus (MNV) as a surrogate for human (HuNoV) in heat inactivation studies. Methods and Results: MNV, hepatitis A virus (HAV) HuNoV genogroup I II (GI GII) specific real‐time quantitative reverse transcription (qRT)‐PCR assays were used to effects exposure (63 72°C) up 10 min water milk. Using culture assays, MNV HAV showed similar reductions infectivity over time. Both GI GII lower log qRT‐PCR titre following than either or HAV. No significant...
Human noroviruses are the most common cause of acute gastroenteritis worldwide. Recently, cell culture systems have been described using either human embryonic intestinal epithelial cells (Int-407) or colorectal adenocarcinoma (Caco-2) growing on collagen-I porous micro carrier beads in a rotating bioreactor under conditions physiological fluid shear. Here, we describe efforts from two independent laboratories to implement this three dimensional (3D) system for replication norovirus. Int-407...
ABSTRACT In July 2006, public health services investigated an outbreak of acute gastroenteritis among staff and visitors a popular ski resort in southern New Zealand. The source the was drinking water supply contaminated by human sewage. virological component investigation played major role confirming outbreak. Drinking water, stream 31 fecal specimens from cases were analyzed for presence norovirus (NoV). Water samples concentrated ultrafiltration, real-time reverse transcription-PCR...
In this study, we developed a triplex real-time reverse transcription-PCR (RT-PCR)-based method that detects and distinguishes between noroviruses belonging to genogroups I, II, III targets the junction regions of open reading frame 1 (ORF1) ORF2. This is first assay include all three RT-PCR-based for detection bovine noroviruses. The was shown be broadly reactive against wide spectrum norovirus genotypes, including GI/1 through GI/7, GII/1 GII/8, GII/10, GII/12, GII/17, in different...
Analysis of complete capsid sequences the emerging norovirus GII.17 Kawasaki 308 from 13 countries demonstrated that they originated a single haplotype since initial emergence in China late 2014. Global spread sublineage SL2 was identified. A new SL3 emerged 2016.
The etiology of an outbreak gastroenteritis in humans cannot always be determined, and ∼25% outbreaks remain unsolved New Zealand. It is hypothesized that novel viruses may account for a proportion cases, new unbiased high-throughput sequencing methods hold promise their detection. Analysis the fecal metagenome can reveal presence viruses, bacteria, parasites which have evaded routine diagnostic testing. Thirty-one samples from 26 unknown occurring Zealand between 2011 2012 were selected de...
Norovirus is estimated to cause 677 million annual cases of gastroenteritis worldwide, resulting in 210,000 deaths. As viral generally self-limiting, clinical samples for epidemiological studies only partially represent circulating noroviruses the population and biased towards severe symptomatic cases. infected individuals from both asymptomatic shed viruses into sewerage system at a high concentration, waste water are useful molecular analysis norovirus genotypes level. Using Illumina MiSeq...