Kate Templeton
A5071196544- Respiratory viral infections research
- SARS-CoV-2 and COVID-19 Research
- Viral gastroenteritis research and epidemiology
- Viral Infections and Immunology Research
- SARS-CoV-2 detection and testing
- COVID-19 Clinical Research Studies
- Pneumonia and Respiratory Infections
- Antimicrobial Resistance in Staphylococcus
- Bacterial Identification and Susceptibility Testing
- Animal Virus Infections Studies
- COVID-19 epidemiological studies
- Influenza Virus Research Studies
- Bacterial Infections and Vaccines
- Hepatitis C virus research
- Reproductive tract infections research
- Hepatitis B Virus Studies
- HIV/AIDS drug development and treatment
- Biosensors and Analytical Detection
- Antibiotic Resistance in Bacteria
- HIV Research and Treatment
- Mycobacterium research and diagnosis
- Infective Endocarditis Diagnosis and Management
- HIV/AIDS Research and Interventions
- Parvovirus B19 Infection Studies
- Cytomegalovirus and herpesvirus research
Edinburgh Royal Infirmary
2016-2025
NHS Lothian
2016-2025
University of Edinburgh
2013-2025
Royal Victoria Hospital
2015-2024
University of Ulster
2015-2024
Western General Hospital
2022-2023
Quadram Institute
2022
Imperial College London
2022
Boston Children's Hospital
2022
National Institute for Health Research
2021
ABSTRACT Four human coronaviruses (HCoV-229E, HCoV-HKU1, HCoV-NL63, and HCoV-OC43) are associated with a range of respiratory outcomes, including bronchiolitis pneumonia. Their epidemiologies clinical characteristics poorly described often reliant on case reports. To address these problems, we conducted large-scale comprehensive screening for all four by analysis 11,661 diagnostic samples collected in Edinburgh, United Kingdom, over 3 years between July 2006 June 2009 using novel four-way...
Vaccines based on the spike protein of SARS-CoV-2 are a cornerstone public health response to COVID-19. The emergence hypermutated, increasingly transmissible variants concern (VOCs) threaten this strategy. Omicron (B.1.1.529), fifth VOC be described, harbours multiple amino acid mutations in spike, half which lie within receptor-binding domain. Here we demonstrate substantial evasion neutralization by BA.1 and BA.2 vitro using sera from individuals vaccinated with ChAdOx1, BNT162b2...
ABSTRACT Laboratory diagnosis of viral respiratory infections is generally performed by virus isolation in cell culture and immunofluorescent assays. Reverse transcriptase PCR now recognized as a sensitive specific alternative for detection RNA viruses. A rapid real-time multiplex assay was developed the influenza B viruses, human syncytial (RSV), parainfluenza 1 (PIV1), PIV2, PIV3, PIV4 two-tube reaction which used molecular beacons to discriminate pathogens. total 358 samples taken over...
The widespread use of antibiotics in association with high-density clinical care has driven the emergence drug-resistant bacteria that are adapted to thrive hospitalized patients. Of particular concern globally disseminated methicillin-resistant Staphylococcus aureus (MRSA) clones cause outbreaks and epidemics associated health care. most rapidly spreading tenacious health-care-associated clone Europe currently is EMRSA-15, which was first detected UK early 1990s subsequently spread...
We report severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike ΔH69/V70 in multiple independent lineages, often occurring after acquisition of receptor binding motif replacements such as N439K and Y453F, known to increase affinity the ACE2 confer antibody escape. In vitro, we show that, although itself is not an evasion mechanism, it increases infectivity associated with enhanced incorporation cleaved into virions. able partially rescue proteins that have acquired Y453F escape...
Entamoeba histolytica, Giardia lamblia, and Cryptosporidium are three of the most important diarrhea-causing parasitic protozoa. For many years, microscopic examination stool samples has been considered to be "gold standard" for diagnosis E. G. C. parvum infections. Recently, more specific sensitive alternative methods (PCR, enzyme-linked immunosorbent assay, direct fluorescent-antibody assay) have introduced all these However, incorporation in a routine diagnostic laboratory...
Background. The frequent lack of a microbiological diagnosis in community-acquired pneumonia (CAP) impairs pathogen-directed antimicrobial therapy. This study assessed the use comprehensive multibacterial, multiviral molecular testing, including quantification, adults hospitalized with CAP. Methods. Clinical and laboratory data were collected for 323 radiologically-confirmed CAP admitted to 2 UK tertiary care hospitals. Sputum (96%) or endotracheal aspirate (4%) specimens cultured as per...
There are thousands of survivors the 2014 Ebola outbreak in west Africa. virus can persist for months immune-privileged sites; however, viral relapse causing life-threatening and potentially transmissible disease has not been described. We report a case late patient who had treated severe with high load (peak cycle threshold value 13.2).A 39-year-old female nurse from Scotland, assisted humanitarian effort Sierra Leone, received intensive supportive treatment experimental antiviral...
Understanding the longitudinal trajectory of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies is crucial for diagnosis prior infection and predicting future immunity.We conducted a analysis disease 2019 convalescent patients, with neutralizing antibody assays SARS-CoV-2 serological assay platforms using spike (S) or nucleocapsid (N) antigens.Sensitivities in diagnosing changed time. One widely used commercial platform that had an initial sensitivity >95% declined to...
In the winter of 2014/15 a novel GII.P17-GII.17 norovirus strain (GII.17 Kawasaki 2014) emerged, as major cause gastroenteritis outbreaks in China and Japan. Since their emergence these viruses have replaced previously dominant GII.4 genotype Sydney 2012 variant some areas Asia but were only detected limited number cases on other continents. This perspective provides an overview available information GII.17 order to gain insight viral host characteristics this genotype. We further discuss...
BackgroundCOVID-19 has affected care home residents internationally, but detailed information on outbreaks is scarce. We aimed to describe the evolution of COVID-19 in all homes one large health region Scotland.MethodsWe did a population analysis testing, cases, and deaths National Health Service (NHS) Lothian UK. obtained data for testing (PCR nasopharyngeal swabs severe acute respiratory syndrome coronavirus 2 [SARS-CoV-2]) (COVID-19-related non-COVID-19-related), we analysed by several...
Abstract Vaccines based on the spike protein of SARS-CoV-2 are a cornerstone public health response to COVID-19. The emergence hypermutated, increasingly transmissible variants concern (VOCs) threaten this strategy. Omicron, fifth VOC be described, harbours 30 amino acid mutations in including 15 receptor-binding domain. Here, we demonstrate substantial evasion neutralisation by Omicron vitro using sera from vaccinated individuals. Importantly, these data mirrored reduction real-world...
Abstract Since its first identification in Scotland, over 1,000 cases of unexplained paediatric hepatitis children have been reported worldwide, including 278 the UK 1 . Here we report an investigation 38 cases, 66 age-matched immunocompetent controls and 21 immunocompromised comparator participants, using a combination genomic, transcriptomic, proteomic immunohistochemical methods. We detected high levels adeno-associated virus 2 (AAV2) DNA liver, blood, plasma or stool from 27 28 cases....
Community-acquired pneumonia (CAP) remains an important cause of morbidity and mortality. Polymerase chain reaction (PCR) has been shown to be more sensitive than current standard microbiological methods and, therefore, may improve the accuracy diagnosis for patients with CAP.Conventional detection techniques multiplex real-time PCR atypical bacteria respiratory viruses were performed on samples collected from 105 adults enrolled in a prospective study. An infiltrate was visible each...
Human bocavirus (HBoV) and PARV4 are newly discovered human parvoviruses. HBoV, which was first detected in respiratory samples, has a potential role the development of disease. The present study compared frequencies, epidemiological profiles, clinical backgrounds HBoV infections with those other virus infections, by evaluating diagnostic samples referred to Specialist Virology Laboratory (SVL) at Royal Infirmary Edinburgh (Edinburgh, United Kingdom).Anonymized subject information were...
ABSTRACT Infections with human parechoviruses (HPeVs) are prevalent in young children and have been associated mild gastroenteritis and, less frequently, meningitis neonatal sepsis. To investigate the involvement of these viruses respiratory disease, a highly sensitive nested PCR was used to screen large archive specimens, collected between January December 2007. Respiratory samples had previously tested for eight viruses, including syncytial virus adenovirus, by PCR. HPeV detected 34 3,844...
ABSTRACT Serology and nucleic acid amplification are the main diagnostic tools for diagnosis of Mycoplasma pneumoniae infection. Since no reference standard is generally accepted, serologic assays M. have not been evaluated on a broad scale. In this study, 12 commercially available (for immunoglobulin G [IgG] IgM) complement fixation test (CFT) were by using DNA detection real-time PCR as “gold standard.” The tested Platelia EIA (Bio-Rad), SeroMP (Savyon), Serion classic (Virion/Serion),...
Hospital-associated infections caused by methicillin-resistant Staphylococcus aureus (MRSA) are a global health burden dominated small number of bacterial clones. The pandemic EMRSA-16 clone (ST36-II) has been widespread in UK hospitals for 20 y, but its evolutionary origin and the molecular basis hospital association unclear. We carried out Bayesian phylogenetic reconstruction on genome sequences 87 S. isolates including 60 27 additional clonal complex 30 (CC30) isolates, collected from...
Human parechoviruses (HPeVs), along with human enteroviruses (HEVs), are associated neonatal sepsis and meningitis. We determined the relative importance of these viruses specific HPeV types involved in development central nervous system-associated disease.A total 1575 cerebrospinal fluid (CSF) samples obtained during 2006-2008 were screened for by means nested polymerase chain reaction. All which results positive typed sequencing viral protein (VP) 3/VP1. Screening HEV was performed...
ABSTRACT The Luminex xTAG Respiratory Virus Panel (RVP) assay has been shown to offer improved diagnostic sensitivity over traditional viral culture methods and have a comparable those of individual real-time nucleic acid tests for respiratory viruses. objective this retrospective study was test new, streamlined version assay, the RVP Fast which requires considerably less run time operator involvement. compared performance with culture, direct fluorescent (DFA), panel single multiplex PCRs...
The frequent lack of a positive and timely microbiological diagnosis in patients with lower respiratory tract infection (LRTI) is an important obstacle to antimicrobial stewardship. Patients are typically prescribed broad-spectrum empirical antibiotics while microbiology results awaited, but, because these often slow, negative, or inconclusive, de-escalation narrow-spectrum agents rarely occurs clinical practice. aim this study was develop evaluate two multiplex real-time PCR assays for the...