A5082549004- Antimicrobial Resistance in Staphylococcus
- Bacterial biofilms and quorum sensing
- Bacterial Identification and Susceptibility Testing
- Streptococcal Infections and Treatments
- Pneumonia and Respiratory Infections
- Bacterial Infections and Vaccines
- Biochemical and Structural Characterization
- Mycobacterium research and diagnosis
- Mathematics and Applications
- Bacteriophages and microbial interactions
- Salmonella and Campylobacter epidemiology
- History and Theory of Mathematics
- Clostridium difficile and Clostridium perfringens research
- Respiratory viral infections research
- Antibiotic Resistance in Bacteria
- Viral gastroenteritis research and epidemiology
- Bacterial Genetics and Biotechnology
- graph theory and CDMA systems
- Genomics and Phylogenetic Studies
- Plant Pathogenic Bacteria Studies
- Microbial Metabolism and Applications
- Tuberculosis Research and Epidemiology
- RNA and protein synthesis mechanisms
- Pneumocystis jirovecii pneumonia detection and treatment
- Vibrio bacteria research studies
University of Mississippi Medical Center
2016-2025
Jackson Memorial Hospital
2010-2024
UK Centre for Ecology & Hydrology
2024
University of Alabama at Birmingham
1996-2024
State Street (United States)
2010-2016
University of California, Irvine
2012
Medtronic (Ireland)
2012
Stryker (United States)
2012
Abbott (Germany)
2012
The Ohio State University Wexner Medical Center
2012
Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of hospital-acquired infections that are becoming increasingly difficult to combat because emerging resistance all current antibiotic classes. The evolutionary origins MRSA poorly understood, no rational nomenclature exists, and there consensus on the number clones or relatedness described from different countries. We resolve these issues provide more thorough precise analysis evolution than has previously been possible....
Since its discovery in the early 2000s, methicillin-resistant Staphylococcus aureus (MRSA) clonal complex 398 (CC398) has become a rapidly emerging cause of human infections, most often associated with livestock exposure. We applied whole-genome sequence typing to characterize diverse collection CC398 isolates (n = 89), including MRSA and methicillin-susceptible S. (MSSA) from animals humans spanning 19 countries four continents. identified 4,238 single nucleotide polymorphisms (SNPs) among...
Staphylococcus aureus is an important human pathogen and represents a growing public health burden owing to the emergence spread of antibiotic-resistant clones, particularly within hospital environment. Despite this, basic questions about evolution population biology species, with regard extent impact homologous recombination, remain unanswered. We address these issues through analysis sequence data obtained from characterization by multilocus typing (MLST) 334 isolates S. aureus, recovered...
Vancomycin-resistant enterococci (VRE) have caused hospital outbreaks worldwide, and the vancomycin-resistance gene (vanA) has crossed genus boundaries to methicillin-resistant Staphylococcus aureus. Spread of VRE, therefore, represents an immediate threat for patient care creates a reservoir mobile resistance genes other, more virulent pathogens. Evolutionary genetics, population structure, geographic distribution 411 VRE vancomycin-susceptible Enterococcus faecium isolates, recovered from...
Abstract Vancomycin-resistant enterococci (VRE) have caused hospital outbreaks worldwide, and the vancomycin-resistance gene (vanA) has crossed genus boundaries to methicillin-resistant Staphylococcus aureus. Spread of VRE, therefore, represents an immediate threat for patient care creates a reservoir mobile resistance genes other, more virulent pathogens. Evolutionary genetics, population structure, geographic distribution 411 VRE vancomycin-susceptible Enterococcus faecium isolates,...
Device-associated infections involving biofilm remain a persistent clinical problem. We recently reported that four methicillin-resistant Staphylococcus aureus (MRSA) strains formed independently of the icaADBC-encoded exopolysaccharide. Here, we report MRSA development was promoted under mildly acidic growth conditions triggered by addition glucose to medium. Loss sortase, which anchors LPXTG-containing proteins peptidoglycan, reduced phenotype. Furthermore introduction mutations in fnbA...
ABSTRACT Five major lineages of methicillin-resistant Staphylococcus aureus (MRSA) have evolved since the introduction methicillin for treatment infections caused by penicillin-resistant S. in 1959. The clones these are responsible vast majority hospital-acquired MRSA disease globally. We constructed high-resolution evolutionary models each lineage using a parsimony approach with 15 partial gene sequences from 147 geographically diverse isolates. On basis models, we infer that has emerged at...
be seriously reconsidered and perhaps abandoned.Possibly
ABSTRACT A multilocus sequence typing (MLST) scheme based on seven housekeeping genes was used to investigate the epidemiology and population structure of Enterococcus faecalis . MLST 110 isolates from different sources geographic locations revealed 55 types that grouped into four major clonal complexes (CC2, CC9, CC10, CC21) by use eBURST. Two these complexes, CC2 are particularly fit in hospital environment, as includes previously described BVE complex identified an alternative CC9...
ABSTRACT Production of icaADBC -encoded polysaccharide intercellular adhesin, or poly- N -acetylglucosamine (PIA/PNAG), represents an important biofilm mechanism in staphylococci. We previously described a glucose-induced, ica -independent four methicillin-resistant Staphylococcus aureus (MRSA) isolates. Here, regulation by NaCl and glucose was characterized 114 MRSA 98 methicillin-sensitive S. (MSSA) isolates from diagnosed device-related infections. NaCl-induced development significantly...
Conjugative transfer and replacement of hundreds kilobases a bacterial chromosome can occur in vitro, but replacements nature are either an order magnitude smaller or involve the movement mobile genetic elements. We discovered that two lineages Staphylococcus aureus, including pandemic methicillin-resistant lineage, were founded by single chromosomal at least approximately 244 557 kb representing 10 20% chromosome, respectively, without obvious involvement The unprecedented natural...
Biofilm formation in tracheal tubes, its bacterial content, and interaction with ventilator gas flow were investigated. At least 50 mg (dry weight) of biofilm was found 30 40 tubes used intensive care patients for 2 h to 10 days. Electron microscopy showed bacteria this layer, quantitative studies that counts could reach up 10(6)/cm tube length. Bacteria cultured from the patient side 18 78 heat moisture exchanger-microbiological filter units removed circuits. Particles shown detach luminal...
Hospital-associated infections caused by methicillin-resistant Staphylococcus aureus (MRSA) are a global health burden dominated small number of bacterial clones. The pandemic EMRSA-16 clone (ST36-II) has been widespread in UK hospitals for 20 y, but its evolutionary origin and the molecular basis hospital association unclear. We carried out Bayesian phylogenetic reconstruction on genome sequences 87 S. isolates including 60 27 additional clonal complex 30 (CC30) isolates, collected from...
We analyzed a representative sample of methicillin-resistant Staphylococcus aureus (MRSA) from 11 European countries (referred to as the HARMONY collection) using three molecular typing methods used within group examine their usefulness for large, multicenter MRSA surveillance networks that use these different laboratory methodologies. isolates were collected based on prevalence in each center and genetic diversity, assessed by pulsed-field gel electrophoresis (PFGE). PFGE groupings (< or =...
The common factor in 13 recent outbreaks of Campylobacter jejuni enteritis was the consumption unpasteurised or incompletely pasteurised milk. C is a commensal alimentary tract milking cows, but it not clear how milk becomes contaminated with organism. Pasteurisation will readily eliminate organism from In England and Wales 3% retailed still unpasteurised, light these findings suggested that only should be sold to public.
The Bengal Bay clone (ST772) is a community-associated and multidrug-resistant Staphylococcus aureus lineage first isolated from Bangladesh India in 2004. In this study, we showed that the emerged virulent progenitor circulating on Indian subcontinent. Its subsequent global transmission was associated with travel or family contact region. ST772 progressively acquired specific resistance elements at limited cost to its fitness continues be exported globally, resulting small-scale community...
Background. The community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) epidemic in the United States is attributed to spread of USA300 clone. An CA-MRSA closely related has occurred northern South America (USA300 Latin-American variant, USA300-LV). Using phylogenomic analysis, we aimed understand relationships between these 2 epidemics.
ABSTRACT Staphylococcus aureus skin infection is a frequent and recurrent problem in children with the common inflammatory disease atopic dermatitis (AD). S. colonizes of majority AD exacerbates disease. The first step during colonization bacterial adhesion to cornified envelope corneocytes outer layer, stratum corneum. Corneocytes from are structurally different normal healthy skin. objective this study was identify proteins that promote adherence corneocytes. strains clonal complexes 1 8...
Clonal complex 5 methicillin-resistant Staphylococcus aureus (CC5-MRSA) includes multiple prevalent clones that cause hospital-associated infections in the Western Hemisphere. Here, we present a phylogenomic study of these MRSA to reveal their phylogeny, spatial and temporal population structure, evolution selected traits. We studied 598 genome sequences, including 409 newly generated from 11 countries Central, North, South America, references Asia Europe. An early-branching CC5-Basal clade...
ABSTRACT Community methicillin-resistant Staphylococcus aureus (CMRSA) strains are being isolated with increasing frequency around the world. In Western Australia CMRSA endemic in geographically remote communities and have been found to belong five different contour-clamped homogeneous electric field (CHEF) electrophoretic patterns. Representatives of each these CHEF patterns compared representative from other Australian states New Zealand. With one exception, all isolates were...
In October and November 1978 two outbreaks of enteritis occurred in the north England. Symptoms lasted to over eight days but no case necessitated admission hospital. Faecal specimens from most patients were found contain thermophilic Campylobacter sp. Inquiry disclosed that all had consumed unpasteurised milk local farms. Examination rectal swabs cattle concerned socks yielded strains sp indistinguishable those isolated patients. It was therefore concluded that, since campylobacters are not...