A5069162354- Antimicrobial Resistance in Staphylococcus
- Bacterial biofilms and quorum sensing
- Bacterial Identification and Susceptibility Testing
- Antibiotic Resistance in Bacteria
- Streptococcal Infections and Treatments
- Mycobacterium research and diagnosis
- Enterobacteriaceae and Cronobacter Research
- Bacteriophages and microbial interactions
- Vibrio bacteria research studies
- Antibiotics Pharmacokinetics and Efficacy
- Pharmaceutical and Antibiotic Environmental Impacts
- Clostridium difficile and Clostridium perfringens research
- Infective Endocarditis Diagnosis and Management
- Nosocomial Infections in ICU
- Plant Pathogenic Bacteria Studies
- Antifungal resistance and susceptibility
- Urinary Tract Infections Management
- Pneumonia and Respiratory Infections
- Microbial infections and disease research
- Cystic Fibrosis Research Advances
- Microbial Metabolism and Applications
- Biochemical and Structural Characterization
- Antibiotic Use and Resistance
- Bacterial Infections and Vaccines
- Respiratory viral infections research
Université Libre de Bruxelles
2015-2024
Antwerp Management School
2024
Universitair Ziekenhuis Brussel
2011-2023
Erasmus Hospital
2013-2022
Laboratoire National de Référence
2018
Istituto Superiore di Sanità
2008
Washington University in St. Louis
1995
Pulsed-fieldgel electrophoresis (PFGE) is the most common genotypic method used in reference and clinical laboratories for typing methicillin-resistant Staphylococcus aureus (MRSA). Many different protocols have been developed that extensive experience with technique established national databases. However, comparabilities of European PFGE MRSA various clones themselves had not addressed until now. This multinational Union (EU) project has first time a database representative epidemic...
Abstract The discovery of antibiotics more than 80 years ago has led to considerable improvements in human and animal health. Although antibiotic resistance environmental bacteria is ancient, pathogens thought be a modern phenomenon that driven by the clinical use 1 . Here we show particular lineages methicillin-resistant Staphylococcus aureus —a notorious pathogen—appeared European hedgehogs pre-antibiotic era. Subsequently, these spread within local hedgehog populations between secondary...
To evaluate the usefulness of phenotypic and genotypic analyses for epidemiologic typing methicillin-resistant Staphylococcus aureus (MRSA), we characterized 64 epidemic MRSA isolates 10 sporadic methicillin-susceptible S. from a university hospital 18 hospitals in different geographical areas. Chromosomal DNA macrorestriction analysis with SstII was resolved by pulsed-field gel electrophoresis compared antibiotype analysis, phage type standard genomic restriction BglII. Indices...
ABSTRACT The susceptibilities of 12 antimicrobial agents for two collections Staphylococcus aureus , isolated in the 1970s and 2006 from poultry, were determined. For eight antibiotics, percentage resistance was significantly higher recent isolates. Ten isolates methicillin resistant had spa types t011 t567, belonging to multilocus sequence type 398. This is first report “livestock-associated” S. healthy poultry.
ABSTRACT Current DNA amplification-based typing methods for bacterial pathogens often lack interlaboratory reproducibility. In this international study, sequence-based of the Staphylococcus aureus protein A gene ( spa , 110 to 422 bp) showed 100% intra- and reproducibility without extensive harmonization protocols 30 blind-coded S. samples sent 10 laboratories. Specialized software automated sequence analysis ensured a common nomenclature.
We analyzed a representative sample of methicillin-resistant Staphylococcus aureus (MRSA) from 11 European countries (referred to as the HARMONY collection) using three molecular typing methods used within group examine their usefulness for large, multicenter MRSA surveillance networks that use these different laboratory methodologies. isolates were collected based on prevalence in each center and genetic diversity, assessed by pulsed-field gel electrophoresis (PFGE). PFGE groupings (< or =...
ABSTRACT We describe the molecular characterization of a multiresistant Pseudomonas aeruginosa clone causing an outbreak in intensive care unit (ICU) tertiary-care university hospital. Analysis included antimicrobial susceptibility profile, O-serotyping, pulsed-field gel electrophoresis, and amplified fragment length polymorphism. Resistance mechanisms were characterized, including production naturally occurring acquired β-lactamases, porin expression, efflux pump systems. Eighteen patients...
Worldwide spread of a limited number Panton-Valentine leucocidin (PVL) -producing methicillin-resistant Staphylococcus aureus (MRSA) clones has been reported in various communities. The objective this study was to describe the molecular characteristics first PVL-positive MRSA strains isolated Belgium.Clinical isolates (n = 41) collected from 2002 2004 Belgian patients were investigated for PVL gene by PCR. genotyped PFGE, staphylococcal cassette chromosome mec (SCCmec) typing, spa sequence...
ABSTRACT Pulsed-field gel electrophoresis (PFGE) of genomic macrorestriction fragments has been used by the Belgian Reference Laboratory for Staphylococci national hospital surveys methicillin-resistant Staphylococcus aureus since 1992. The sequencing polymorphic X region protein A gene ( spa typing) offers significant advantages over PFGE in terms speed, ease interpretation, and exportability. To validate its potential use surveillance, we evaluated robustness typing compared with that...
Genome macrorestriction fingerprinting with XbaI and DraI was used to analyze the relatedness of 166 Pseudomonas aeruginosa isolates collected from 31 cystic fibrosis patients over a 1- 20-month period correlate their genotype patterns resistance 14 antimicrobial agents. Quantitative comparison intra- interpatient similarities P. disclosed two discrete ranges that clearly discriminated subclonal variation (> 80% relatedness) clonal diversity (10 70% relatedness). Cloning-derived mutants...
Objectives16S rRNA methylase-mediated high-level resistance to aminoglycosides has been reported recently in clinical isolates of Gram-negative bacilli only from a limited number countries. This study was conducted investigate the occurrence this type Enterobacteriaceae two Belgian hospitals and characteristics strains.
We report on the evolution and epidemiology of carbapenem non-susceptible Enterobacteriaceae (CNSE) including carbapenemase-producing (CPE) in Belgium between January 2007 April 2011. Significantly higher numbers CNSE CPE were detected 2010 2011 compared to years 2009. The majority patients carrying did not have history travel abroad. rapid emergence autochthonous strains since warrants strengthened epidemiological surveillance at national level.
ABSTRACT Staphylococcus epidermidis is a major cause of catheter-related bloodstream infections (CRBSIs). Recent studies suggested the existence well-adapted, highly resistant, hospital-associated S. clones. The molecular epidemiology in Belgian hospitals and community has not been explored yet. We compared set 33 isolates causing CRBSI hospitalized patients with commensal isolates. factors analyzed included resistance to antibiotics genetic diversity as determined by pulsed-field gel...
ABSTRACT Methicillin-resistant Staphylococcus aureus isolates lacking mec genes ( n = 32), collected from Belgian hospitals, were characterized for their β-lactamase production and the presence of mutations in pbp genes, pbp4 promoter, involved penicillin-binding protein 4 overproduction gdpP yjbH ). Twelve hyperproducers (BHPs), while 12 non-BHP might produce an incomplete GdpP protein. Most showed nucleotide missense genes. A few also promoter.
Abstract Objectives In August 2018, a public health alert was issued in Belgium regarding clusters of impetigo cases caused by the epidemic European fusidic acid-resistant clone (EEFIC) Staphylococcus aureus. As result, Belgian national reference centre (NRC) commissioned to update epidemiology S. aureus causing community-onset skin and soft tissues infection (CO-SSTI) assess proportion EEFIC among them. Methods For 1 year, clinical laboratories were asked send their first three isolated...
Pulsed-field gel electrophoresis of DNA macrorestriction fragments (macrorestriction analysis) allows epidemiologic typing and delineation genetic relatedness methicillin-resistant Staphylococcus aureus (MRSA) by indexing variations in the global chromosome architecture. Polymerase chain reaction (PCR)-mediated genome fingerprinting can also discriminate MRSA strains detecting locally variable motifs. To assess correlation between these methods, 48 epidemic collected from 20 hospitals over a...
During a PCR-based surveillance study of β-lactam resistance, 125 multidrug-resistant (MDR) Acinetobacter baumannii isolates were obtained from 18 hospitals in Belgium January 2008 to December 2009. Nine GES-positive A. detected at 6 Belgian hospitals. DNA sequencing the bla(GES) genes identified GES-11, GES-12, and novel variant GES-14, which differs GES-11 by single amino acid substitution (Gly170Ser). All index travel associated originated patients transferred Turkey (n = 2), Egypt...
Surveillance of hospital-acquired methicillin-resistant Staphylococcus aureus (HA-MRSA) infections has shown the emergence and spread several epidemic MRSA clones over past 10 years in Belgium. Whether these have been imported from abroad or else arisen locally via staphylococcal cassette chromosome mec (SCCmec) acquisition by successful methicillin-susceptible S. (MSSA) is unknown.We determined PFGE, spa typing, multi-locus sequence typing (MLST) agr group analysis genetic relatedness 103...
ObjectivesTo investigate the presence of extended-spectrum β-lactamases (ESBLs) among Pseudomonas aeruginosa clinical isolates referred to two Belgian reference laboratories.