A5078874316- Antimicrobial Resistance in Staphylococcus
- Bacterial biofilms and quorum sensing
- Biosimilars and Bioanalytical Methods
- Advanced Biosensing Techniques and Applications
- Islamic Finance and Banking Studies
- Gender, Labor, and Family Dynamics
- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- ICT Impact and Policies
- Immune Cell Function and Interaction
- HIV Research and Treatment
- Dialysis and Renal Disease Management
- Antibiotic Resistance in Bacteria
- Microfinance and Financial Inclusion
- SARS-CoV-2 detection and testing
- Bacterial Genetics and Biotechnology
- Labor market dynamics and wage inequality
- Mycobacterium research and diagnosis
- COVID-19 Clinical Research Studies
- Taxation and Compliance Studies
- Crystal structures of chemical compounds
- Renal function and acid-base balance
- Infectious Diseases and Mycology
- HIV/AIDS Research and Interventions
- Clinical Laboratory Practices and Quality Control
The University of Melbourne
2015-2024
Peter Doherty Institute
2015-2024
University of Chicago
2013-2024
Monash Health
2020-2024
California Polytechnic State University
2023-2024
American Society For Engineering Education
2024
University of Rochester Medical Center
2024
University of Illinois Chicago
2024
Emory University
2024
Temple University
2007-2023
We posit that household decision-making over fertility is characterized by moral hazard since most contraception can only be perfectly observed the woman. Using an experiment in Zambia varied whether women were given access to contraceptives alone or with their husbands, we find husbands 19 percent less likely seek family planning services, 25 use concealable contraception, and 27 more give birth. However, report a lower subjective well-being, suggesting psychosocial cost of making...
The multidrug-resistant bacterium Enterococcus faecium has become increasingly tolerant to the alcohols in hospital disinfectants.
Build more capacity, and stretch what we already have
AbstractWe identified potential epigenetic biomarkers for chronic kidney disease progression by comparing site-specific DNA methylation levels in more than 14,000 genes between African American and Hispanic diabetes patients with end stage renal (ESRD) without nephropathy. We 187 that are differentially methylated the two groups on at least CpG sites each gene extracted from saliva. Of whose mean differed groups, 39 genes, or closely related family members, have been reported to be involved...
Engineering of extracellular vesicles (EVs) without affecting biological functions remains a challenge, limiting the broad applications EVs in biomedicine. Here, we report method to equip with various functional agents, including fluorophores, drugs, lipids, and bio-orthogonal chemicals, an efficient controlled manner by engineering parental cells membrane fusogenic liposomes, while keeping intact. As demonstration how this can be applied, prepared containing azide-lipids, conjugated them...
There is an unmet medical need for pruritus associated with chronic kidney disease, a distressing complication characterized by generalized and persistent itch affecting 20% to 40% of patients undergoing hemodialysis. Here we report the results phase 2 trial evaluating efficacy safety novel peripherally restricted kappa opioid receptor agonist, difelikefalin, in adult hemodialysis pruritus.In this study, 174 moderate-to-severe were randomly assigned receive difelikefalin (0.5, 1.0, or 1.5...
Introduction. The SARS-CoV-2 pandemic of 2020 has resulted in unparalleled requirements for RNA extraction kits and enzymes required virus detection, leading to global shortages. This necessitated the exploration alternative diagnostic options alleviate supply chain issues. Aim. To establish validate a reverse transcription loop-mediated isothermal amplification (RT- LAMP) assay detection from nasopharyngeal swabs. Methodology. We used commercial RT-LAMP mastermix OptiGene combination with...
Multidrug-resistant bacterial pathogens like vancomycin-resistant Enterococcus faecium (VREfm) are a critical threat to human health1. Daptomycin is last-resort antibiotic for VREfm infections with novel mode of action2, but which resistance has been widely reported unexplained. Here we show that rifaximin, an unrelated used prophylactically prevent hepatic encephalopathy in patients liver disease3, causes cross-resistance daptomycin VREfm. Amino acid changes arising within the RNA...
To determine the influence of host genetics on human immunodeficiency virus (HIV) type 1 infection, we examined 94 repeatedly exposed seronegative (ES) individuals for polymorphisms in multiple genes and compared results with those 316 HIV-1-seropositive 425 HIV-1-seronegative individuals. The frequency homozygous C-C chemokine receptor (CCR) 5- Delta 32 was higher ES (3.2%) than (0.0%; P=.012). However, CCR5-59029A, CCR2-64I, stromal cell-derived factor (SDF)-1-3'A, RANTES (regulated...
Over 400 professionals representing pharmaceutical companies, CROs, and multiple regulatory agencies participated in the 6th Workshop on Recent Issues Bioanalysis (WRIB). Like previous sessions, this event was format of a practical, focused, highly interactive informative workshop aiming for high-quality, improved compliance scientific excellence. Numerous 'hot' topics bioanalysis both small large molecules were shared discussed, leading to consensus recommendations among panelists attendees...
The 5th Workshop on Recent Issues in Bioanalysis (WRIB) was organized by the Calibration and Validation Group as a 2-day full immersion workshop for pharmaceutical companies, CROs regulatory agencies to discuss, review, share perspectives, provide potential solutions agree upon consistent approach recent issues bioanalysis of both small large molecules. High quality, better compliance regulations scientific excellence are foundation this workshop. As previous editions significant event,...
Using positron emission tomography imaging, we determined the hepatic concentrations and hepatobiliary transport of [ 11 C]rosuvastatin (RSV; i.v. injection) in absence ( n = 6) presence 4 cyclosporin A (CsA; infusion) following a therapeutic dose unlabeled RSV (5 mg, p.o.) healthy human volunteers. The sinusoidal uptake, efflux, biliary efflux clearance (CL; mL/minute) C]RSV, estimated through compartment modeling were 1,205.6 ± 384.8, 16.2 11.2, 5.1 1.8, respectively 6). CsA (blood...
A skewed tryptophan metabolism has been reported in patients with lupus. Here, we investigated the mechanisms by which it occurs lupus-susceptible mice, and how metabolites exacerbate T cell activation. Metabolomic analyses demonstrated that is differentially catabolized lupus mice compared to controls microbiota played a role this skewing. There was no evidence for differential expression of catabolic enzymes further supporting major contribution However, isolated cells processed...
The 2013 7th Workshop on Recent Issues in Bioanalysis was held Long Beach, California, USA, where close to 500 professionals from pharmaceutical and biopharmaceutical companies, CROs regulatory agencies convened discuss current topics of interest bioanalysis. These 'hot' topics, which covered both small large molecules, were the starting point for fruitful exchanges knowledge, sharing ideas among speakers, panelists attendees. discussions led specific recommendations pertinent bioanalytical...
Abstract WalKR (YycFG) is the only essential two-component regulator in human pathogen Staphylococcus aureus . regulates peptidoglycan synthesis, but this function alone does not explain its essentiality. Here, to further understand function, we investigate a suppressor mutant that arose when activity was impaired; histidine tyrosine substitution (H271Y) cytoplasmic Per-Arnt-Sim (PAS CYT ) domain of kinase WalK. Introducing WalK H271Y mutation into wild-type S. activates regulon. Structural...
Vaccinating the world's population quickly in a pandemic has enormous health and economic benefits. We analyze problem faced by governments determining scale structure of procurement for vaccines. alternative approaches to procurement, arguing that buyers should directly fund manufacturing capacity shoulder most risk failure while maintaining some direct incentives speed. analyzed optimal portfolio vaccine investments countries with different characteristics as well implications...
Coagulase-negative staphylococci (CoNS), such as Staphylococcus capitis , are major causes of bloodstream infections in neonatal intensive care units (NICUs). Recently, a distinct clone S. (designated NRCS-A) has emerged an important pathogen NICUs internationally.
The opportunistic human pathogen
Microglial activation is emerging as an important etiologic factor and therapeutic target in neurodegenerative neuroinflammatory diseases. Techniques have been lacking, however, for measuring the different components of microglial independently vivo. We describe a method proliferation rates vivo using heavy water (2H2O) labeling, its application screening drugs that suppress neuro-inflammation. Brain microglia were isolated by flow cytometry F4/80+, CD11b+, CD45(low) cells, 2H enrichment DNA...
Increasingly, commercial immunoassay kits are used to support drug discovery and development. Longitudinally consistent kit performance is crucial, but the degree which reagents characterized by manufacturers not standardized, nor approaches users adapt them evaluate their through validation prior use. These factors can negatively impact data quality. This paper offers a systematic approach assessment, method adaptation of for quantification biomarkers in development, expanding upon previous...
Staphylococcus epidermidis is a major cause of hospital-acquired infections, especially those related to implanted medical devices. Understanding how S. causes disease and devising ways combat these infections have been hindered by an inability genetically manipulate clinically significant hospital-adapted strains. Here, we provide the first comprehensive analyses barriers uptake foreign DNA in demonstrate that are distinct from described for aureus . Using insights, efficient approach...