A5039347550- Biosensors and Analytical Detection
- Vibrio bacteria research studies
- Legionella and Acanthamoeba research
- Salmonella and Campylobacter epidemiology
- Hepatitis Viruses Studies and Epidemiology
- Viral gastroenteritis research and epidemiology
- Advanced biosensing and bioanalysis techniques
- SARS-CoV-2 detection and testing
- Bacterial Identification and Susceptibility Testing
- Hepatitis C virus research
- Advanced Chemical Sensor Technologies
- Antibiotic Resistance in Bacteria
- Aquaculture disease management and microbiota
- Oral microbiology and periodontitis research
- Viral Infections and Immunology Research
- Pharmaceutical and Antibiotic Environmental Impacts
- Listeria monocytogenes in Food Safety
Peter Doherty Institute
2015-2024
The University of Melbourne
2015-2024
ABSTRACT Whole-genome sequencing (WGS) has emerged as a powerful tool for comparing bacterial isolates in outbreak detection and investigation. Here we demonstrate that WGS performed prospectively national epidemiologic surveillance of Listeria monocytogenes the capacity to be superior our current approaches using pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), variable-number tandem-repeat analysis (MLVA), binary typing, serotyping. Initially 423 L. underwent...
Introduction. The SARS-CoV-2 pandemic of 2020 has resulted in unparalleled requirements for RNA extraction kits and enzymes required virus detection, leading to global shortages. This necessitated the exploration alternative diagnostic options alleviate supply chain issues. Aim. To establish validate a reverse transcription loop-mediated isothermal amplification (RT- LAMP) assay detection from nasopharyngeal swabs. Methodology. We used commercial RT-LAMP mastermix OptiGene combination with...
Vibrio alginolyticus causes vibriosis of marine vertebrates, invertebrates, and humans, while there have been several reports multidrug resistance in V. alginolyticus, carbapenem is rare. strain AUSMDU00064140 was isolated Melbourne, Australia, from imported prawns. Routine genomic surveillance detected the presence a full-length blaNDM-1 gene, subsequently shown to be collocated with additional acquired antimicrobial genes on cassette largest chromosome, flanked by mobilization gene...
The shedding patterns of Salmonella spp. and MLVA profiles enterica subspecies (I) serotype 1,4,[5],12:i:- were monitored in a 12-month longitudinal observational study five pig herds to inform management; provide indications potential hazard load at slaughter; assist evaluation for use by animal public health practitioners. Twenty pooled faecal samples, stratified age group, collected quarterly. When was cultured, multiple colonies characterized serotyping where S. Typhimurium-like serovars...
Fundamental to effective Legionnaires' disease outbreak control is the ability rapidly identify environmental source(s) of causative agent,
2. Abstract Introduction The SARS-CoV-2 pandemic of 2020 has resulted in unparalleled requirements for RNA extraction kits and enzymes required virus detection, leading to global shortages. This necessitated the exploration alternative diagnostic options alleviate supply chain issues. Aim To establish validate a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay detection from nasopharyngeal swabs. Methodology We used commercial RT-LAMP mastermix OptiGene Ltd...
ABSTRACT Hepatitis A virus (HAV) infections are an increasing public health concern in low-endemicity regions due to outbreaks from food-borne and sustained transmission among vulnerable groups, including persons experiencing homelessness, those who inject drugs, men have sex with men, which is further compounded by aging, unvaccinated populations. DNA sequence characterization of HAV for source tracking performed comparing small subgenomic the virus. While this approach has been successful...
ABSTRACT Fundamental to effective Legionnaires’ disease outbreak control is the ability rapidly identify environmental source(s) of causative agent, Legionella pneumophila . Genomics has revolutionised pathogen surveillance but L. a complex ecology and population structure that can limit source inference based on standard core genome phylogenetics. Here we present powerful machine learning approach assigns geographical outbreaks more accurately than current comparisons. Models were developed...
Abstract Hepatitis A virus (HAV) infections are an increasing public health concern in low-endemicity regions due to outbreaks from foodborne and sustained transmission among vulnerable groups, including persons experiencing homelessness, those who inject drugs, men have sex with (MSM), which is further compounded by aging, unvaccinated populations. DNA sequence characterisation of HAV for source tracking performed comparing small subgenomic the virus. While this approach has been successful...