A5079572315- Microbial infections and disease research
- Salmonella and Campylobacter epidemiology
- Antibiotic Resistance in Bacteria
- Bacteriophages and microbial interactions
- Antimicrobial Resistance in Staphylococcus
- Bacterial Identification and Susceptibility Testing
- Pharmaceutical and Antibiotic Environmental Impacts
- Aquaculture disease management and microbiota
- Animal Nutrition and Physiology
- Viral gastroenteritis research and epidemiology
- Antibiotics Pharmacokinetics and Efficacy
- Bacterial biofilms and quorum sensing
- Meat and Animal Product Quality
- Vibrio bacteria research studies
- Plant Virus Research Studies
- Listeria monocytogenes in Food Safety
- Streptococcal Infections and Treatments
- Bacterial Genetics and Biotechnology
- Bacterial Infections and Vaccines
- Clostridium difficile and Clostridium perfringens research
- Escherichia coli research studies
- Veterinary medicine and infectious diseases
- Genomics and Phylogenetic Studies
- Plant Pathogenic Bacteria Studies
- Antimicrobial agents and applications
Justus-Liebig-Universität Gießen
2019-2025
University of Veterinary Medicine Hannover, Foundation
2010-2019
Faktor 10 – Institut für nachhaltiges Wirtschaften gemeinnützige
1999-2019
University of Veterinary Medicine Vienna
2010-2014
Friedrich-Loeffler-Institut
2007-2009
Institut für Tier-, Natur- und Umweltethik
2006
Institute of Pathology Celle
2001
A novel multidrug resistance phenotype mediated by the Cfr rRNA methyltransferase is observed in Staphylococcus aureus and Escherichia coli. The cfr gene has previously been identified as a phenicol lincosamide on plasmids isolated from spp. of animal origin recently shown to encode that modifies 23S at A2503. Antimicrobial susceptibility testing shows S. E. coli strains expressing exhibit elevated MICs number chemically unrelated drugs. named PhLOPSA for following drug classes: Phenicols,...
A total of 302 chloramphenicol-resistant Staphylococcus isolates were screened for the presence florfenicol/chloramphenicol resistance genes fexA and cfr their localization on mobile genetic elements. Of 114 from humans, only a single aureus isolate showed an elevated MIC to florfenicol, but did not carry either known genes, or fexA. In contrast, 11 188 staphylococci animal sources considered florfenicol resistant carried (one isolate), (five isolates), both isolates). nine cases we...
Abstract The discovery of antibiotics more than 80 years ago has led to considerable improvements in human and animal health. Although antibiotic resistance environmental bacteria is ancient, pathogens thought be a modern phenomenon that driven by the clinical use 1 . Here we show particular lineages methicillin-resistant Staphylococcus aureus —a notorious pathogen—appeared European hedgehogs pre-antibiotic era. Subsequently, these spread within local hedgehog populations between secondary...
Salmonella Pullorum, the causative agent of pullorum disease, posing a significant threat to global production poultry meat and eggs. However, existing detection methods have substantial limitations in efficiency accuracy. Herein, we developed genomic deletion-targeted TaqMan qPCR assay for identification enabling precise differentiation from other serovars. The assay's limit was 5 copies/μL plasmid 4 CFU/μL bacterial DNA. Furthermore, collected 676 chicken samples an established infection...
Summary The gene product of cfr from Staphylococcus sciuri confers resistance to chloramphenicol, florfenicol and clindamycin in spp. Escherichia coli . Cfr is not similar any other known chloramphenicol determinant. Comparative investigation E. with without a plasmid‐encoded showed decreased drug binding ribosomes the presence Cfr. As chloramphenicol/florfenicol have partly overlapping sites on ribosome, most likely explanation that modifies RNA site. This hypothesis was supported by...
The 16.5-kbp plasmid pSCFS1 from Staphylococcus sciuri mediated combined resistance to chloramphenicol and florfenicol. gene responsible for this property, cfr, was cloned sequenced. amino acid sequence of the Cfr protein revealed no homology known acetyltransferases or efflux proteins involved in and/or florfenicol other whose functions are known.
ABSTRACT Twenty-five distinct tetracycline-resistant gram-negative bacteria recovered from four Chilean fish farms with no history of recent antibiotic use were examined for the presence tetracycline resistance ( tet ) genes. Sixty percent isolates carried 1 22 known genes examined. The distribution was as follows. (A) gene found in six isolates. (B) two isolates, including first description genus Brevundimonas . Two (34) and genes, Pseudomonas Serratia (H) which includes genera Moraxella...
Objectives: Methicillin-resistant Staphylococcus aureus (MRSA) isolates from pet animals were characterized and compared with human clonal complexes most prevalent in Central Europe.
Methicillin-resistant Staphylococcus aureus clonal lineage ST398 and methicillin-susceptible ST9 strains have their main reservoir in swine but can colonize cause infections humans. The phenicol/lincosamide/oxazolidinone/pleuromutilin/streptogramin A multidrug resistance gene cfr was detected isolates of both lineages, rendering a spread to humans with exposure farming possible.
Paratyphoid avian salmonellosis is considered one of the leading causes poultry death, resulting in significant economic losses to industries worldwide. In China, especially Shandong province, producer products, several recurrent outbreaks have been reported during last decade where precise causal agent remains unknown. Moreover, establishment earlier and more accurate recognition pathogens a key factor prevent further dissemination resistant and/or hypervirulent clones. Here, we aim use...
Invasive nontyphoidal Salmonella (iNTS) causes extraintestinal infections with ~15% case fatality in many countries. However, the mechanism by which iNTS emerged China remains unaddressed. We conducted clinical investigations of infection recurrent treatment failure, caused underreported enterica serovar Livingstone (SL). Genomic epidemiology demonstrated five clades SL population and suggested that international animal feed trade was a likely vehicle for their introduction into China, as...
Understanding changes in pathogen behavior (e.g. increased virulence, a shift transmission channel) is critical for the public health management of emerging infectious diseases. Genome degradation via gene depletion or inactivation recognized as pathoadaptive feature evolving with host. However, little known about exact role genome affecting pathogenic behavior, and underlying molecular detail has yet to be examined. Using large-scale global avian-restricted Salmonella genomes spanning more...
Objectives: A Salmonella enterica serovar Infantis isolate of avian origin was investigated for the presence gene qnrS, its transferability and association with other resistance genes. Methods: The susceptibility to antimicrobial agents plasmid content. Hybridization experiments PCR assays were performed identify genes while transformation conjugation studies conducted show their transferability. quinolone resistance-determining regions gyrA, gyrB, parC parE sequenced. Moreover, extended...
During a study of florfenicol-resistant porcine staphylococci from Denmark, the genes cfr and fexA were detected in chromosomal DNA or on plasmids Staphylococcus hyicus, warneri, simulans. A novel variant phenicol resistance transposon Tn558 was ca. 43-kb plasmid pSCFS6 S. warneri simulans isolates. Sequence analysis 22,010-bp segment revealed that new harbored an additional gene region integrated into tnpC reading frame. This consisted clindamycin exporter lsa(B) for combined to phenicols,...
Contaminated poultry meat is considered to be the main source of human infection with Campylobacter spp., a pathogen that asymptomatically colonizes broiler chickens during fattening and contaminates carcasses slaughter. To prevent or reduce colonization flocks applying different organic acids, especially in combinations, via feed drinking water seems promising approach. However, only very few combinations acids have been tested for their antibacterial efficacy against spp. Therefore, vitro...
The Staphylococcus lentus plasmid pSCFS2 carries a novel florfenicol-chloramphenicol resistance gene, designated fexA, encoding protein of 475 amino acids with 14 transmembrane domains. FexA differs from all previously known proteins involved in the efflux chloramphenicol and florfenicol. Induction fexA expression by florfenicol occurs via translational attenuation.
ABSTRACT A florfenicol resistance gene almost identical to floR of Salmonella enterica serovar Typhimurium DT104 was detected on 110- 125-kb plasmids in Escherichia coli isolates animal origin. Analysis the flanking regions one showed that they were different from those encountered S. DT104.
Objectives: The multiresistance plasmid pSCFS1 from Staphylococcus sciuri was sequenced completely and analysed with regard to its gene organization the putative role of a novel ABC transporter in antimicrobial resistance.
The florfenicol-chloramphenicol exporter gene fexA is part of the novel transposon Tn558 from Staphylococcus lentus. Similarities between and Tn554 aureus included arrangement transposase genes tnpA to -C an att554-like target sequence. Circular forms were detected suggest functional activity this transposon.
The lack of differentiation between viable and nonviable bacterial cells limits the implementation PCR-based methods for routine diagnostic approaches. Recently, combination a quantitative real-time PCR (qPCR) ethidium monoazide (EMA) or propidium (PMA) pretreatment has been described to circumvent this disadvantage. In regard suitability approach Campylobacter spp., conflicting results have reported. Thus, we compared suitabilities EMA PMA in various concentrations viability qPCR method....