A5007726682- Salmonella and Campylobacter epidemiology
- Antibiotic Resistance in Bacteria
- Reproductive Physiology in Livestock
- Animal health and immunology
- Antimicrobial Resistance in Staphylococcus
- Bacterial Identification and Susceptibility Testing
- Vibrio bacteria research studies
- Escherichia coli research studies
- Trace Elements in Health
- Viral gastroenteritis research and epidemiology
- Ruminant Nutrition and Digestive Physiology
- Microbial infections and disease research
- Listeria monocytogenes in Food Safety
- Food Safety and Hygiene
- Genetic and phenotypic traits in livestock
- Bacterial biofilms and quorum sensing
- Animal Disease Management and Epidemiology
- Bacteriophages and microbial interactions
- Aquaculture disease management and microbiota
- Probiotics and Fermented Foods
- Metabolism and Genetic Disorders
- Identification and Quantification in Food
- Viral Infections and Immunology Research
- Insect and Pesticide Research
- Viral Infections and Vectors
Justus-Liebig-Universität Gießen
2003-2025
University of Bern
2024
University Hospital of Bern
2024
IDEXX Laboratories (Germany)
2011-2022
Rijnstate Hospital
2022
Klinikum St. Georg
2021
University of Veterinary Medicine Hannover, Foundation
2016-2020
Klinik und Poliklinik für Kinder- und Jugendmedizin
2001-2016
Swiss Federal University for Vocational Education and Training SFUVET
2011
Zentrum für Kinderheilkunde
2007
Salmonella Pullorum, the causative agent of pullorum disease, posing a significant threat to global production poultry meat and eggs. However, existing detection methods have substantial limitations in efficiency accuracy. Herein, we developed genomic deletion-targeted TaqMan qPCR assay for identification enabling precise differentiation from other serovars. The assay's limit was 5 copies/μL plasmid 4 CFU/μL bacterial DNA. Furthermore, collected 676 chicken samples an established infection...
Paratyphoid avian salmonellosis is considered one of the leading causes poultry death, resulting in significant economic losses to industries worldwide. In China, especially Shandong province, producer products, several recurrent outbreaks have been reported during last decade where precise causal agent remains unknown. Moreover, establishment earlier and more accurate recognition pathogens a key factor prevent further dissemination resistant and/or hypervirulent clones. Here, we aim use...
Invasive nontyphoidal Salmonella (iNTS) causes extraintestinal infections with ~15% case fatality in many countries. However, the mechanism by which iNTS emerged China remains unaddressed. We conducted clinical investigations of infection recurrent treatment failure, caused underreported enterica serovar Livingstone (SL). Genomic epidemiology demonstrated five clades SL population and suggested that international animal feed trade was a likely vehicle for their introduction into China, as...
ABSTRACT Pestiviruses belong to the family Flaviviridae , and their genome is a single-stranded RNA of positive polarity encoding one large polyprotein which further processed into mature proteins. Noncytopathogenic (noncp) strains pestivirus bovine viral diarrhea virus (BVDV) can establish persistent infection. In persistently infected animals, noncp BVDVs occasionally acquire mutations in nonstructural protein 2 (NS2) that give rise cytopathogenic (cp) BVDV variants, and, eventually, lead...
Abstract Background Although the Netherlands is a country with low endemic level of methicillin-resistant Staphylococcus aureus (MRSA), national MRSA surveillance has been in place since 1989. In 2003 livestock emerged as major reservoir and currently livestock-associated (clonal complex CC398) make up 25% all isolates. To assess possible transfer resistant strains or resistance genes, obtained from humans animals were characterized detail. Methods The sequenced genomes 6327 isolates 332...
ABSTRACT Polyprotein processing control is a crucial step in the life cycle of positive-strand RNA viruses. Recently, vital autoprotease generating an essential viral replication factor was identified such virus, namely, pestivirus bovine diarrhea virus. Surprisingly, activity this protease, which resides nonstructural protein 2 (NS2), diminishes early after infection, resulting limitation replication. Here, we describe that cellular chaperone termed Jiv (J-domain interacting with protein)...
Blood plasma and hepatic parameters were identified that describe the differences between metabolically robust or vulnerable dairy cows grouped according to their past health status. Data from a field study on used which challenged selected had milk fat percentage of >4.5 mg/g protein ratio >1.5 in previous early lactation. The either classified as based occurrence various metabolic (re)production disorders lactations. liver tissue samples collected week 3 ante partum (a.p.) (-3 wk), 4 (+4...
Avian cellulitis in broilers, caused by avian pathogenic Escherichia coli, is a major cause for carcass rejections during meat inspection, resulting significant economic losses. In this study, we analysed E. coli isolates obtained from broiler chickens affected their genetic relatedness and antimicrobial resistance phenotype genotype. The objective was to determine whether there clonal spread or these clinical differ. For purpose, isolated swab samples collected diseased broilers across 77...
From 2004 until 2016, 21 Père David's deer (Elaphurus davidianus) have died for unknown reason at Zoo Duisburg. These deer, also known as milu, succumbed from a myopathy that occurred seasonally in autumn and spring. The clinical signs shown by the animals closely resembles those of disease called equine atypical (EAM), which is formerly horses. cause EAM Europe was found ingestion hypoglycin A, contained samaras seedlings sycamore maple tree (Acer pseudoplatanus). To test hypothesis...
Background Bacteria that have acquired antimicrobial resistance, in particular ESBL-producing Enterobacteriaceae, are an important healthcare concern. Therefore, transmission routes and risk factors of interest, especially for the carriage E. coli. Since there is enhanced pig slaughterhouse employees to carry associated with animal contact as potential factor, present study investigated occurrence Enterobacteriaceae poultry employees. Due higher level resistant primary production than...
Extended-spectrum β-lactamase (ESBL)-producing Escherichia coli and methicillin-resistant Staphylococcus aureus (MRSA) represent major healthcare concerns. The role of wildlife in the epidemiology these bacteria is unclear. purpose this study was to determine their prevalence wild boars Germany characterize individual isolates. A total 375 fecal samples 439 nasal swabs were screened for presence ESBL-/AmpC-E. MRSA, respectively. associations seven demographic anthropogenic variables with...
Food products of animal origin can serve as a vehicle for Staphylococcus (S.) aureus, facultative pathogen involved in variety diseases. As result, international trade and illegal transportation foodstuffs facilitate the distribution S. aureus over long distances. In this study, we investigated isolates recovered from meat confiscated passengers returning non-EU countries at two German airports samples legally imported meats countries. The aim was to characterize regard their genetic...
Antimicrobial resistance is a global health threat that involves complex, opaque transmission processes in the environment. In particular, wildlife appears to function as reservoir and vector for antimicrobial-resistant bacteria well genes. present study, occurrence of Escherichia coli was determined marine mammals various fish species North Baltic Seas. Rectal or faecal swabs were collected from 66 live-caught stranded 40 specimens. The antimicrobial phenotypes genotypes isolated E. using...
Efficient proteolytic release of nonstructural protein 3 (NS3) from the viral polyprotein is considered to be crucial for cytopathogenicity pestiviruses. Here we describe a novel cytopathogenic (cp) bovine diarrhea virus strain (BVDV CP8) with complex insertion composed and cell-derived sequences, including two fragments cellular J-domain Jiv (J-domain interacting protein) located in N-terminal region polyprotein. BVDV CP8 expresses fusion 513 amino acids addition complete set proteins. This...
Though imports of products animal origin into the European Union (EU) have to comply with legal requirements and quality standards community, food consignment rejections at external EU borders been increasing in recent years. This study explored microbiological metrics according national target critical values valid for samples consumer level 498 fresh poultry meat 136 pork filets from consignments subjected physical checks during clearing border inspection post Hamburg harbour between...
Food products of animal origin brought into the EU from third countries, both legally and illegally, can harbor foodborne pathogens such as Salmonella enterica. In this study, we examined five S. enterica isolates recovered either imported chicken meat (n = 3) or confiscated air travel passengers arriving in Germany 2). The were serotyped further characterized by antimicrobial susceptibility testing, PCR-detection sequencing genes associated with resistances, macrorestriction analysis....
Phosphorus (P) deficiency in early lactating dairy cows is receiving increased attention because of incentives aiming at curtailing environmental pollution with P by reducing dietary ruminant diets. An in-vitro study using bovine hepatocytes incubated for 7 days phosphate (Pi) concentrations 0.9, 1.8 or 2.7 mmol/L, and an in-vivo feeding late pregnant diets either adequate (0.28% 0.44% DM ante-partum post-partum respectively) low content (0.15% 0.20% from 4 weeks before to after calving were...
Raw beef imported into the European Union (EU) from third countries must fulfill same legal requirements as products EU member states. Recently, microbiological quality of 100 raw samples was investigated. Samples were quantitatively analyzed according to DIN ISO standard methods for aerobic mesophilic colony counts (ACC), Enterobacteriaceae, Escherichia coli, Listeria monocytogenes, coagulase-positive staphylococci and qualitatively L. monocytogenes Salmonella spp. also screened presence...
Conditions to which the cardiac graft is exposed during transplantation with donation after circulatory death (DCD) can trigger recruitment of macrophages that are either unpolarized (M0) or pro-inflammatory (M1) as well release extracellular vesicles (EV). We aimed characterize effects M0 and M1 macrophage-derived EV administration on post-ischaemic functional recovery glucose metabolism using an isolated rat heart model DCD. Isolated hearts were subjected 20 min aerobic perfusion, followed...