A5038215233- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Viral Infections and Immunology Research
- Plant Virus Research Studies
- Bacteriophages and microbial interactions
- SARS-CoV-2 and COVID-19 Research
- RNA and protein synthesis mechanisms
- Virus-based gene therapy research
- Viral Infections and Vectors
- Plant and Fungal Interactions Research
- Mosquito-borne diseases and control
- Viral Infections and Outbreaks Research
- CRISPR and Genetic Engineering
- Respiratory viral infections research
- Bacterial Genetics and Biotechnology
- Polyomavirus and related diseases
- Animal Disease Management and Epidemiology
- RNA modifications and cancer
- RNA regulation and disease
- Hepatitis C virus research
- COVID-19 epidemiological studies
- Evolution and Genetic Dynamics
- RNA Research and Splicing
- Virology and Viral Diseases
- interferon and immune responses
Leiden University Medical Center
2016-2025
Lomonosov Moscow State University
2015-2024
Bioengineering Center
2020
Leiden University
1995-2015
Moscow State University
2006-2015
European Molecular Biology Laboratory
2009
National Institutes of Health
1999-2004
Centre National de la Recherche Scientifique
2004
Architecture et Fonction des Macromolécules Biologiques
2004
National Center for Biotechnology Information
2004
The present outbreak of a coronavirus-associated acute respiratory disease called coronavirus 19 (COVID-19) is the third documented spillover an animal to humans in only two decades that has resulted major epidemic. Coronaviridae Study Group (CSG) International Committee on Taxonomy Viruses, which responsible for developing classification viruses and taxon nomenclature family Coronaviridae, assessed placement human pathogen, tentatively named 2019-nCoV, within Coronaviridae. Based phylogeny,...
Abstract The present outbreak of lower respiratory tract infections, including distress syndrome, is the third spillover, in only two decades, an animal coronavirus to humans resulting a major epidemic. Here, Coronavirus Study Group (CSG) International Committee on Taxonomy Viruses, which responsible for developing official classification viruses and taxa naming (taxonomy) Coronaviridae family, assessed novelty human pathogen tentatively named 2019-nCoV. Based phylogeny, taxonomy established...
During the summer of 2012, in Jeddah, Saudi Arabia, a hitherto unknown coronavirus (CoV) was isolated from sputum patient with acute pneumonia and renal failure (1, 2). The isolate provisionally called human Erasmus Medical Center (EMC) (3). Shortly thereafter, September same type virus, named England 1, recovered severe respiratory illness who had been transferred Gulf region Middle East to London, United Kingdom (4) (GenBank accession no. KC164505.2). onset new disease traced back an even...
In the course of systematic analysis protein sequences containing purine NTP-binding motif, a new superfamily was delineated which included 25 established or putative halicases Escherichia coli, yeast, insects, mammals, pox- and herpesvirusea, yeast mitochondriel plasmid three groups positive strand RNA viruses. These proteins contained 7 distinct highly conserved segments two corresponded to "A" "B" sites NTP–binding motif. Typical an abridged consensus for site, GxGKS/T, instead classical...
ABSTRACT A novel human coronavirus (HCoV-EMC/2012) was isolated from a man with acute pneumonia and renal failure in June 2012. This report describes the complete genome sequence, organization, expression strategy of HCoV-EMC/2012 its relation known coronaviruses. The contains 30,119 nucleotides at least 10 predicted open reading frames, 9 which are to be expressed nested set seven subgenomic mRNAs. Phylogenetic analysis replicase gene coronaviruses completely sequenced genomes showed that...
A novel coronavirus is the causative agent of current epidemic severe acute respiratory syndrome (SARS). Coronaviruses are exceptionally large RNA viruses and employ complex regulatory mechanisms to express their genomes. Here, we determined sequence SARS (SARS-CoV), isolate Frankfurt 1, characterized key elements protein functions involved in viral genome expression. Important mechanisms, such as (discontinuous) synthesis eight subgenomic mRNAs, ribosomal frameshifting post-translational...
Replication of the giant RNA genome severe acute respiratory syndrome (SARS) coronavirus (CoV) and synthesis as many eight subgenomic (sg) mRNAs are mediated by a viral replicase-transcriptase outstanding complexity that includes an essential endoribonuclease activity. Here, we show CoV replicative machinery, unlike other viruses, also uses exoribonuclease (ExoN) activity, which is associated with nonstructural protein (nsp) 14. Bacterially expressed forms SARS-CoV nsp14 were shown to act on...
Significance The 2003 severe acute respiratory syndrome (SARS) epidemic and recent emergence of Middle East highlight the potential lethality zoonotic coronavirus infections in humans. No specific antiviral treatment options are available. Coronaviruses possess largest known RNA virus genomes encode a complex replication machinery consisting 16 viral nonstructural proteins (nsps). Our study reveals that SARS-coronavirus polymerase (nsp12) needs to associate with nsp7 nsp8 activate its...
Computer-assisted comparison of the nonstructural polyprotein hepatitis E virus (HEV) with proteins other positive-strand RNA viruses allowed identification following putative functional domains: (i) RNA-dependent polymerase, (ii) helicase, (iii) methyltransferase, (iv) a domain unknown function ("X" domain) flanking papain-like protease domains in polyproteins animal viruses, and (v) cysteine distantly related to rubella (RubV). Comparative analysis polymerase helicase sequences belonging...
The family Picornaviridae comprises small non-enveloped viruses with RNA genomes of 6.7 to 10.1 kb, and contains >30 genera >75 species. Most the known picornaviruses infect mammals birds, but some have also been detected in reptiles, amphibians fish. Many are important human veterinary pathogens may cause diseases central nervous system, heart, liver, skin, gastrointestinal tract or upper respiratory tract. transmitted by faecal-oral routes. This is a summary International Committee on...
The Polyomaviridae constitute a family of small DNA viruses infecting variety hosts. In humans, polyomaviruses can cause infections the central nervous system, urinary tract, skin, and possibly respiratory tract. Here we report identification new human polyomavirus in plucked facial spines heart transplant patient with trichodysplasia spinulosa, rare skin disease exclusively seen immunocompromized patients. spinulosa-associated (TSV) genome was amplified through rolling-circle amplification...
Computer-assisted comparisons of the large proteins involved in replication viral RNA have revealed a novel domain located near N termini these and conserved throughout so-called 'Sindbis-like' supergroup positive-strand viruses. This encompasses four distinct motifs, with motifs I, II IV containing an invariant His residue, AspXXArg signature Tyr respectively. Each two groups viruses within this supergroup, 'altovirus' group (alphaviruses, tobamoviruses, tobraviruses, hordeiviruses,...
Statistically significant similarity was revealed between amino acid sequences of NTP-binding pattern-containing domains which are among the most conserved protein segments in dissimilar groups ss and dsDNA viruses (papova-, parvo-, geminiviruses P4 bacteriophage), RNA (picorna-, como- nepoviruses) with small genomes. Within aligned 100-120 residues, three highly sequence have been identified, i.e. 'A' 'B' motifs pattern, a third, C-terminal motif 'C', not described previously. The proteins...