A5013451849- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Enzyme Structure and Function
- Bacterial Genetics and Biotechnology
- Glycosylation and Glycoproteins Research
- Genomics and Phylogenetic Studies
- Enzyme Catalysis and Immobilization
- Insect and Pesticide Research
- Protein Structure and Dynamics
- Neurobiology and Insect Physiology Research
- Vibrio bacteria research studies
- Escherichia coli research studies
- Enzyme Production and Characterization
- Pancreatic function and diabetes
- Insect and Arachnid Ecology and Behavior
- Olfactory and Sensory Function Studies
- Biochemical and Molecular Research
- Viral gastroenteritis research and epidemiology
- Photosynthetic Processes and Mechanisms
- Microbial Metabolic Engineering and Bioproduction
- Cancer Research and Treatments
- Protein purification and stability
- Healthcare and Venom Research
- Plant Virus Research Studies
Centre National de la Recherche Scientifique
2016-2025
Aix-Marseille Université
2016-2025
Institut de Microbiologie de la Méditerranée
2022-2025
University College Cork
2018-2025
Laboratoire d'Ingénierie des Systèmes Macromoléculaires
2022-2025
APC Microbiome Institute
2022-2024
Architecture et Fonction des Macromolécules Biologiques
2014-2023
Alpha MOS (France)
2022-2023
Laboratoire de Photochimie et d'Ingénierie Macromoléculaire
2023
Building Bridges
2016
Replication of the giant RNA genome severe acute respiratory syndrome (SARS) coronavirus (CoV) and synthesis as many eight subgenomic (sg) mRNAs are mediated by a viral replicase-transcriptase outstanding complexity that includes an essential endoribonuclease activity. Here, we show CoV replicative machinery, unlike other viruses, also uses exoribonuclease (ExoN) activity, which is associated with nonstructural protein (nsp) 14. Bacterially expressed forms SARS-CoV nsp14 were shown to act on...
Abstract CRISPR-Cas systems are bacterial anti-viral systems, and viruses (bacteriophages, phages) can carry anti-CRISPR (Acr) proteins to evade that immunity. Acrs also fine-tune the activity of CRISPR-based genome-editing tools. While prevalent in phages capable lying dormant a CRISPR-carrying host, their orthologs have been observed only infrequently virulent phages. Here we identify AcrIIA6, an Acr encoded 33% Streptococcus thermophilus phage genomes. The X-ray structure AcrIIA6 displays...
The recently identified etiological agent of the severe acute respiratory syndrome (SARS) belongs to Coronaviridae (CoV), a family viruses replicating by poorly understood mechanism. Here, we report crystal structure at 2.7-Å resolution nsp9, hitherto uncharacterized subunit SARS-CoV replicative polyproteins. We show that nsp9 is single-stranded RNA-binding protein displaying previously unreported, oligosaccharide/oligonucleotide fold-like fold. presence this type has not been detected in...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe 2.46 .ANG. Resolution Structure of the Pancreatic Lipase-Colipase Complex Inhibited by a C11 Alkyl PhosphonateMarie-Pierre Egloff, Frank Marguet, Gerard Buono, Robert Verger, Christian Cambillau, and Herman van TilbeurghCite this: Biochemistry 1995, 34, 9, 2751–2762Publication Date (Print):March 7, 1995Publication History Published online1 May 2002Published inissue 7 March 1995https://doi.org/10.1021/bi00009a003RIGHTS & PERMISSIONSArticle...
While most organisms grow at temperatures ranging between 20 and 50 °C, many archaea a few bacteria have been found capable of withstanding close to 100 or beyond, such as <i>Pyrococcus</i> <i>Aquifex</i>. Here we report the results two independent large scale unbiased approaches identify global protein properties correlating with an extreme thermophile lifestyle. First, performed comparative proteome analyses using 30 complete genome sequences from three kingdoms. A difference proportions...
Macro domains constitute a protein module family found associated with specific histones and proteins involved in chromatin metabolism. In addition, small number of animal RNA viruses, such as corona- toroviruses, alphaviruses, hepatitis E virus, encode macro for which, however, structural functional information is extremely limited. Here, we characterized the from Semliki Forest severe acute respiratory syndrome coronavirus (SARS-CoV). The crystal structure SARS-CoV domain was determined at...
Pheromone-binding proteins (PBPs), located in the sensillum lymph of pheromone-responsive antennal hairs, are thought to transport hydrophobic pheromones chemosensory membranes olfactory neurons. It is currently unclear what role PBPs may play recognition and discrimination species-specific pheromones. We have investigated binding properties specificity fromMamestra brassicae (MbraPBP1), Antheraea polyphemus (ApolPBP1), Bombyx mori (BmorPBP), a hexa-mutant MbraPBP1 (Mbra1-M6), mutated at...