A5004359762- Bacterial Genetics and Biotechnology
- Escherichia coli research studies
- Bacteriophages and microbial interactions
- Vibrio bacteria research studies
- RNA and protein synthesis mechanisms
- Antibiotic Resistance in Bacteria
- ATP Synthase and ATPases Research
- Galectins and Cancer Biology
- Mitochondrial Function and Pathology
- Genomics and Phylogenetic Studies
- Enzyme Structure and Function
- Helicobacter pylori-related gastroenterology studies
- Advanced Electron Microscopy Techniques and Applications
- Photosynthetic Processes and Mechanisms
- Bacterial biofilms and quorum sensing
- Monoclonal and Polyclonal Antibodies Research
- Lipid Membrane Structure and Behavior
- DNA Repair Mechanisms
- Force Microscopy Techniques and Applications
- Immune Response and Inflammation
- Legume Nitrogen Fixing Symbiosis
- Microbial Community Ecology and Physiology
- melanin and skin pigmentation
- Yersinia bacterium, plague, ectoparasites research
- Bacterial Infections and Vaccines
Institut Européen de Chimie et Biologie
2003-2025
Université de Bordeaux
2017-2025
Centre National de la Recherche Scientifique
2016-2025
Institut Polytechnique de Bordeaux
2017-2025
Laboratoire de Microbiologie Fondamentale et Pathogénicité
2018-2024
Institut Pasteur
2010-2017
Centre de Gestion Scientifique
2017
Laboratoire des Biomolécules
2016
Birkbeck, University of London
2007-2009
Institute of Structural and Molecular Biology
2008-2009
Type IV secretion systems (T4SSs) are important virulence factors used by Gram-negative bacterial pathogens to inject effectors into host cells or spread plasmids harboring antibiotic resistance genes. We report the 15 angstrom resolution cryo–electron microscopy structure of core complex a T4SS. The is composed three proteins, each present in 14 copies and forming ∼1.1-megadalton two-chambered, double membrane–spanning channel. double-walled, with component apparently spanning large part...
Natural genetic transformation is widely distributed in bacteria and generally occurs during a genetically programmed differentiated state called competence. This process promotes genome plasticity adaptability Gram-negative Gram-positive bacteria. Transformation requires the binding internalization of exogenous DNA, mechanisms which are unclear. Here, we report discovery pilus at surface competent Streptococcus pneumoniae cells. Type IV-like pilus, primarily composed ComGC pilin, required...
Abstract Secreted exopolysaccharides present important determinants for bacterial biofilm formation, survival, and virulence. Cellulose secretion typically requires the concerted action of a c-di-GMP-responsive inner membrane synthase (BcsA), an accessory membrane-anchored protein (BcsB), several additional Bcs components. Although BcsAB catalytic duo has been studied in great detail, its interplay with co-expressed subunits remains enigmatic. Here we show that E . coli proteins partake...
Strength in numbers Human cluster of differentiation 20 (CD20) is expressed on malignant B cells and the target therapeutic antibodies used cancer immunotherapy. Kumar et al. now present structures that explain why so-called type I efficiently activate complement pathway to kill cells, whereas II do not. Type each bind two CD20 dimers form clusters facilitate binding a component pathway. The second-generation antibody ofatumumab has molecular features make it more efficient at clustering...
Abstract Homologous recombination (HR) is a central process of genome biology driven by conserved recombinase, which catalyses the pairing single-stranded DNA (ssDNA) with double-stranded to generate D-loop intermediate. Bacterial RadA HR effector acting RecA recombinase promote ssDNA integration. The mechanism this RadA-mediated assistance unknown. Here, we report functional and structural analyses from human pathogen Streptococcus pneumoniae . found facilitate RecA-driven over long genomic...
Bacteria have evolved macromolecular machineries that secrete effectors and toxins to survive thrive in diverse environments. The type VI secretion system (T6SS) is a contractile machine related Myoviridae phages. It composed of phage tail-like structure inserted the bacterial cell envelope by membrane complex (MC) comprising TssJ, TssL TssM proteins. We previously reported low-resolution negative-stain electron microscopy enteroaggregative Escherichia coli MC proposed rotational 5-fold...
Dopamine is an essential brain neuromodulator involved in reward and motor control. Dopaminergic (DA) neurons project to most areas, with particularly dense innervation the striatum. DA varicosities bind target striatal synapses form dopamine hub (DHS). However, basic features of release sites are still largely unknown. Here we studied ultrastructure fluorescent glutamatergic (GLU) synaptosomes isolated from striatum adult mice cryo-correlative light electron microscopy cryo-electron...
Abstract Acetaldehyde–alcohol dehydrogenase (AdhE) enzymes are a key metabolic enzyme in bacterial physiology and pathogenicity. They convert acetyl-CoA to ethanol via an acetaldehyde intermediate during fermentation anaerobic environment. This two-step reaction is associated NAD + regeneration, essential for glycolysis. The bifunctional AdhE conserved all kingdoms but also more phylogenetically distant microorganisms such as green microalgae. It found oligomeric form called spirosomes,...
Abstract Bacteria have evolved toxins to outcompete other bacteria or hijack host cell pathways. One broad family of bacterial polymorphic gathers multidomain proteins with a modular organization, comprising C-terminal toxin domain fused N-terminal that adapts the delivery apparatus. Polymorphic include bacteriocins, contact-dependent growth inhibition systems, and specialized Hcp, VgrG, PAAR Rhs Type VI secretion (T6SS) components. We recently described characterized Tre23, T6SS-associated...
Type VII secretion systems mediate protein extrusion from Gram-positive bacteria and are classified as T7SSa T7SSb in Actinobacteria Firmicutes , respectively. Despite the genetic divergence of T7SSb, high degree structural similarity their WXG100 substrates suggests similar mechanisms.
Type IV secretion (T4S) systems translocate DNA and protein effectors through the double membrane of Gram-negative bacteria. The paradigmatic T4S system in Agrobacterium tumefaciens is assembled from 11 VirB subunits VirD4. Two subunits, VirB9 VirB7, form an important stabilizing complex outer membrane. We describe here NMR structure a between C-terminal domain homolog TraO (TraO(CT)), bound to VirB7-like TraN plasmid pKM101. TraO(CT) forms beta-sandwich around which winds. Structure-based...