A5080756371- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- Advanced Electron Microscopy Techniques and Applications
- RNA modifications and cancer
- Enzyme Structure and Function
- Bacterial Genetics and Biotechnology
- Protein Structure and Dynamics
- RNA Interference and Gene Delivery
- X-ray Diffraction in Crystallography
- Telomeres, Telomerase, and Senescence
- Crystallization and Solubility Studies
- Heat shock proteins research
- Photosynthetic Processes and Mechanisms
- Viral gastroenteritis research and epidemiology
- Genomics and Phylogenetic Studies
- Plant Virus Research Studies
- Ion channel regulation and function
- Metalloenzymes and iron-sulfur proteins
- Alzheimer's disease research and treatments
- Electron and X-Ray Spectroscopy Techniques
- Acute Lymphoblastic Leukemia research
- T-cell and Retrovirus Studies
- DNA Repair Mechanisms
- ATP Synthase and ATPases Research
- Escherichia coli research studies
Birkbeck, University of London
2015-2025
Institute of Structural and Molecular Biology
2013-2025
University of Chicago
2024
St Petersburg University
2023
Novosibirsk State Technical University
2023
Russian State Agrarian Correspondence University
2022
Institute of Theoretical and Experimental Biophysics
2012-2019
University College London
2008-2019
International Medical Research (Germany)
2019
Research & Development Corporation
2019
The cross-β amyloid form of peptides and proteins represents an archetypal widely accessible structure consisting ordered arrays β-sheet filaments. These complex aggregates have remarkable chemical physical properties, the conversion normally soluble functional forms into structures is linked to many debilitating human diseases, including several common age-related dementia. Despite their importance, however, fibrils proved be recalcitrant detailed structural analysis. By combining...
Type IV secretion systems (T4SSs) are important virulence factors used by Gram-negative bacterial pathogens to inject effectors into host cells or spread plasmids harboring antibiotic resistance genes. We report the 15 angstrom resolution cryo–electron microscopy structure of core complex a T4SS. The is composed three proteins, each present in 14 copies and forming ∼1.1-megadalton two-chambered, double membrane–spanning channel. double-walled, with component apparently spanning large part...
Bacterial conjugation is the fundamental process of unidirectional transfer DNAs, often plasmid from a donor cell to recipient cell1. It primary means by which antibiotic resistance genes spread among bacterial populations2,3. In Gram-negative bacteria, mediated large transport apparatus-the conjugative type IV secretion system (T4SS)-produced and embedded in both its outer inner membranes. The T4SS also elaborates long extracellular filament-the pilus-that essential for DNA transfer4,5....
Protein misfolding and aggregation cause serious degenerative conditions such as Alzheimer’s, Parkinson, prion diseases. Damage to membranes is thought be one of the mechanisms underlying cellular toxicity a range amyloid assemblies. Previous studies have indicated that fibrils can membrane leakage elicit damage, these effects are enhanced by fragmentation fibrils. Here we report direct 3D visualization damage specific interactions lipid bilayer with amyloid-like formed in vitro from β 2...
Human low density lipoproteins (LDL) are the major cholesterol carriers in blood. Elevated concentration of LDL is a risk factor for atherosclerotic disease. Purified particles appear heterogeneous images obtained with 400-kV electron cryomicroscope. Using multivariate statistical and cluster analyses, an ensemble randomly oriented particle has been subdivided into homogeneous subpopulations, largest subset was used three-dimensional reconstruction. In contrast to general belief that below...