A5030313195- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Bacteriophages and microbial interactions
- Advanced Biosensing Techniques and Applications
- Protein purification and stability
- Viral Infectious Diseases and Gene Expression in Insects
- Celiac Disease Research and Management
- T-cell and B-cell Immunology
- RNA and protein synthesis mechanisms
- Transgenic Plants and Applications
- Biosimilars and Bioanalytical Methods
- Advanced Fluorescence Microscopy Techniques
- Advanced biosensing and bioanalysis techniques
- Photochromic and Fluorescence Chemistry
- CAR-T cell therapy research
- Nanofabrication and Lithography Techniques
- Neuroscience and Neuropharmacology Research
- Enzyme Structure and Function
- Galectins and Cancer Biology
- vaccines and immunoinformatics approaches
- Autoimmune Bullous Skin Diseases
- Photosynthetic Processes and Mechanisms
- Microscopic Colitis
- Biofuel production and bioconversion
- Advanced Proteomics Techniques and Applications
Specifica (United States)
2018-2025
IQVIA (United States)
2024-2025
Q2 Solutions (United States)
2022-2024
Los Alamos National Laboratory
2009-2021
Los Alamos Medical Center
2012-2015
Bioscience Research
2014
Center for Integrated Nanotechnologies
2008-2012
Louisiana State University
2012
University of California System
2011
University of New Mexico
2009
To save millions of dollars and dramatically improve reproducibility, protein-binding reagents must be defined by their sequences produced as recombinant proteins, say Andrew Bradbury, Andreas Plückthun 110 co-signatories.
Cerebellar granule cells undergo apoptosis in culture after deprivation of potassium and serum. During this process we found that tau, a neuronal microtubule-associated protein plays key role the maintenance architecture, pathology which correlates with intellectual decline Alzheimer's disease, is cleaved. The final product cleavage soluble dephosphorylated tau fragment 17 kDa unable to associate microtubules accumulates perikarya dying cells. appearance inhibited by both caspase calpain...
Trends in increased tuberculosis infection and a fatality rate of approximately 23% have necessitated the search for alternative biomarkers using newly developed postgenomic approaches. Here we provide systematic analysis Mycobacterium (Mtb) by directly profiling its gene products. This combines high-throughput proteomics computational approaches to elucidate globally expressed complements three subcellular compartments (the cell wall, membrane, cytosol) Mtb. We report identifications 1044...
To determine coeliac disease prevalence by an anti-transglutaminase antibody assay in a large paediatric population; to evaluate acceptance of the screening programme, dietary compliance, and long term health effects.Cross-sectional survey 3188 schoolchildren (aged 6-12) prospective follow up diagnosed cases. Main outcome measures were: defined intestinal biopsy or positivity both human tissue transglutaminase anti-endomysium antibodies HLA DQ2-8 positive subjects; percentage children whose...
OBJECTIVE: Tissue transglutaminase is the autoantigen recognized by sera of celiac patients. An enzyme-linked immunosorbent assay (ELISA) based on guinea-pig tissue was recently used to measure serum antibodies for diagnosis disease. We determine sensitivity and specificity an ELISA test use human recombinant transglutaminase, compared with guinea pig IgA antiendomysium antibodies. METHODS: Serum samples were tested from 65 patients intestinal biopsy proven disease, 10 Crohn's 150 healthy...
PC3 is an immediate early gene induced by nerve growth factor in PC12 cells, a cell line derived from tumor of the adrenal medulla that undergoes neuronal differentiation presence factor. This induction independent new protein synthesis as it can occur cycloheximide. also with similar kinetics, but at lower levels, membrane depolarization (both vivo and vitro) epidermal It much levels fibroblast interleukin 6. In found expressed tissues, such brain embryonic day 13.5, placenta, amnion,...
Monoclonal antibodies are commonly assumed to be monospecific, but anecdotal studies have reported genetic diversity in antibody heavy chain and light genes found within individual hybridomas. As the prevalence of such has never been explored, we analyzed 185 random hybridomas, a large multicenter dataset. The hybridomas were not biased towards those with cloning difficulties or known additional chains. Of evaluated, 126 (68.1%) contained no productive chains, while remaining 59 (31.9%) one...
Because of its great potential for diversity, the immunoglobulin heavy-chain complementarity-determining region 3 (HCDR3) is taken as an antibody molecule's most important component in conferring binding activity and specificity. For this reason, HCDR3s have been used unique identifiers to investigate adaptive immune responses vivo characterize vitro selection outputs where display systems were employed. Here, we show that many different can be identified within a target-specific population...
We have identified a class of tau fragments inducing apoptosis in different cellular contexts, including human teratocarcinoma‐derived cell line (NT2 cells) representing committed neuronal precursors. found transition point inside the molecule beyond which lose their ability to induce apoptosis. This is located around one putative caspase‐3 cleavage sites. only site that can be effectively used by vitro, releasing C‐terminal 19 amino acids tau. These results establish as substrate for an...
Celiac disease (CD) is an intestinal malabsorption characterized by intolerance to cereal proteins accompanied immunological responses dietary gliadins and tissue transglutaminase, autoantigen located in the endomysium. Tissue transglutaminase belongs family of enzymes that catalyze protein cross-linking reactions constitutively expressed many tissues as well being activated during apoptosis. The role eliciting immune response CD how linked primary reaction are still unclear. In this work,...
Tissue transglutaminase is the autoantigen recognized by sera of celiac patients. An enzyme-linked immunosorbent assay (ELISA) based on guinea-pig tissue was recently used to measure serum antibodies for diagnosis disease. We determine sensitivity and specificity an ELISA test use human recombinant transglutaminase, compared with guinea pig IgA antiendomysium antibodies.Serum samples were tested from 65 patients intestinal biopsy proven disease, 10 Crohn's 150 healthy blood donors.Human...
We have developed a high-throughput protein expression and interaction analysis platform that combines cDNA phage display library selection massive gene sequencing using the 454 platform. A of open reading frame (ORF) fragments was created from mRNA derived different tissues. This used to study network enzyme transglutaminase 2 (TG2), multifunctional involved in regulation cell growth, differentiation apoptosis, associated with many pathologies. After two rounds panning TG2 we assayed...
Therapeutic antibodies must have "drug-like" properties. These include high affinity and specificity for the intended target, biological activity, additional characteristics now known as "developability properties": long-term stability resistance to aggregation when in solution, thermodynamic prevent unfolding, expression yields facilitate manufacturing, low self-interaction, among others. Sequence-based liabilities may affect one or more of these characteristics. Improving developability a...
Phage display technology involves the of proteins or peptides, as coat protein fusions, on surface a phage phagemid particles. Using standard technology, helper are essential for replication and assembly particles, during library production biopanning. We have eliminated need to add by using 'bacterial packaging cell lines' that provide same functions. These lines contain M13-based plasmids express which assemble particles efficiently phage, but without contamination. This results in...
Many cellular activities are controlled by post-translational modifications, the study of which is hampered lack specific reagents due in large part to their ubiquitous and non-immunogenic nature. Although antibodies against specifically modified sequences relatively easy obtain, it extremely difficult derive recognizing modifications independently sequence context surrounding modification. In this study, we examined possibility selecting such from phage antibody libraries using...