A5022332197- Celiac Disease Research and Management
- Microscopic Colitis
- Eosinophilic Esophagitis
- Galectins and Cancer Biology
- Digestive system and related health
- Helicobacter pylori-related gastroenterology studies
- Diabetes and associated disorders
- Monoclonal and Polyclonal Antibodies Research
- Autoimmune Bullous Skin Diseases
- Gastrointestinal disorders and treatments
- Transgenic Plants and Applications
- Immunodeficiency and Autoimmune Disorders
- Microbial Metabolites in Food Biotechnology
- Blood properties and coagulation
- Bartonella species infections research
- Gut microbiota and health
- Viral gastroenteritis research and epidemiology
- Food Allergy and Anaphylaxis Research
- Diet and metabolism studies
- Medical Coding and Health Information
- Viral Infections and Immunology Research
- Viral Infections and Vectors
- Food composition and properties
- Healthcare Systems and Public Health
- Whipple's Disease and Interleukins
IRCCS Materno Infantile Burlo Garofolo
2015-2024
University of Trieste
2013-2022
BioCruces Health research Institute
2019
University of the Basque Country
2019
Health and Human Development (2HD) Research Network
2009
Istituti di Ricovero e Cura a Carattere Scientifico
1993-2005
University of Maryland, Baltimore
1998-2005
Clínica Santa María
2004
Royal Hallamshire Hospital
2002
Sheffield Hallam University
2002
<h3>Background</h3> Celiac disease (CD) is an immune-mediated enteropathic condition triggered in genetically susceptible individuals by the ingestion of gluten. Although common Europe, CD thought to be rare United States, where there are no large epidemiologic studies its prevalence. The aim this study was determine prevalence at-risk and not-at-risk groups States. <h3>Methods</h3> Serum antigliadin antibodies anti–endomysial (EMA) were measured. In EMA-positive subjects, human tissue...
Objective. Little is known about the interaction of gliadin with intestinal epithelial cells and mechanism(s) through which crosses barrier. We investigated whether has any immediate effect on zonulin release signaling. Material methods. Both ex vivo human small intestines cell monolayers were exposed to gliadin, changes in paracellular permeability monitored presence absence antagonism. Zonulin binding, cytoskeletal rearrangement, zonula occludens-1 (ZO-1) redistribution evaluated by...
AbstractBackground: Recent epidemiologic studies in Europe using antigliadin (AGA) and anti-endomysium antibodies (AEA) for initial screening have shown that the overall prevalence of celiac disease (CD) is about 1:300. There are no comparable scientific data USA, where CD considered rare. The main aim this study was to determine increased AEA healthy blood donors USA. Methods: Sera from 2000 were screened IgG AGA IgA with an enzyme-linked immunosorbent assay test. All those levels,...
Increased intestinal permeability has been observed in numerous human autoimmune diseases, including type-1 diabetes (T1D) and its' animal model, the BB-wor diabetic prone rat. We have recently described zonulin, a protein that regulates intercellular tight junctions. The objective of this study was to establish whether zonulin-dependent increased plays role pathogenesis T1D. In BB diabetic-prone rat model T1D, intraluminal zonulin levels were elevated 35-fold compared control...
To determine coeliac disease prevalence by an anti-transglutaminase antibody assay in a large paediatric population; to evaluate acceptance of the screening programme, dietary compliance, and long term health effects.Cross-sectional survey 3188 schoolchildren (aged 6-12) prospective follow up diagnosed cases. Main outcome measures were: defined intestinal biopsy or positivity both human tissue transglutaminase anti-endomysium antibodies HLA DQ2-8 positive subjects; percentage children whose...
OBJECTIVE: Tissue transglutaminase is the autoantigen recognized by sera of celiac patients. An enzyme-linked immunosorbent assay (ELISA) based on guinea-pig tissue was recently used to measure serum antibodies for diagnosis disease. We determine sensitivity and specificity an ELISA test use human recombinant transglutaminase, compared with guinea pig IgA antiendomysium antibodies. METHODS: Serum samples were tested from 65 patients intestinal biopsy proven disease, 10 Crohn's 150 healthy...
Celiac disease (CD) is an intestinal malabsorption characterized by intolerance to cereal proteins accompanied immunological responses dietary gliadins and tissue transglutaminase, autoantigen located in the endomysium. Tissue transglutaminase belongs family of enzymes that catalyze protein cross-linking reactions constitutively expressed many tissues as well being activated during apoptosis. The role eliciting immune response CD how linked primary reaction are still unclear. In this work,...
Tissue transglutaminase is the autoantigen recognized by sera of celiac patients. An enzyme-linked immunosorbent assay (ELISA) based on guinea-pig tissue was recently used to measure serum antibodies for diagnosis disease. We determine sensitivity and specificity an ELISA test use human recombinant transglutaminase, compared with guinea pig IgA antiendomysium antibodies.Serum samples were tested from 65 patients intestinal biopsy proven disease, 10 Crohn's 150 healthy blood donors.Human...
Background Celiac disease (CD) is an autoimmune gastrointestinal disorder characterized by the presence of anti-transglutaminase 2 (TG2) and anti-gliadin antibodies. Amongst neurological dysfunctions associated with CD, ataxia represents most common one. Methods We analyzed immunohistochemistry, anti-neural reactivity serum from 20 CD patients. To determine role anti-TG2 antibodies in ataxia, two single chain variable fragments (scFv), isolated a phage-display IgA antibody library, were...
Vibrio cholerae-derived zonula occludins toxin (Zot) is a multifunctional protein that reversibly disassembles intestinal tight junctions (tjs). Zot structure-function analysis has mapped this activity to aa 288–293, named AT1002. AT1002 reduced transepithelial electrical resistance across rat small intestine, ex vivo, as did and its processed mature form, ΔG. increased in vivo permeability sugar tracers, whereas scrambled control peptides not. Binding barrier assays proteinase activated...
We report here the design of a novel immunosensor and its application for celiac disease diagnosis, based on an electrogenerated chemiluminescence (ECL) readout, using membrane-templated gold nanoelectrode ensembles (NEEs) as detection platform. An original sensing strategy is presented by segregating spatially initial electrochemical reaction location immobilized biomolecules where ECL finally emitted. The recognition scaffold following: tissue transglutaminase (tTG) capturing agent...
<h3>Introduction</h3> Despite climate change being recognised as the greatest health threat of 21st century, current medical education curricula do not reflect urgency crisis. Preparing for climate-related repercussions requires educational institutions to disseminate planetary knowledge in a systematic way. We sought evaluate extent literature on inclusion undergraduate and postgraduate guide curricular development. <h3>Methods analysis</h3> A scoping review is undertaken with search...
Abstract Background Association of the interleukin-23 receptor ( IL23R ) with inflammatory bowel disease (IBD) has been confirmed in several populations. also associates psoriasis, suggesting that gene may be an important candidate for many chronic diseases. Methods We studied association single-nucleotide variants IBD Swedish patients, both Crohn's (CD) and ulcerative colitis (UC) subsets. The same genetic were Finnish patients psoriasis or celiac disease, Hungarian Italian disease. Results...
Anti-transglutaminase antibodies are the diagnostic markers of coeliac disease. A role is suggested for infectious agents in production anti-transglutaminase antibodies. The aim was to measure positive antibody levels children with diseases and compare immunological biological characteristics derived from these that patients. Two hundred twenty-two suffering were enrolled prospectively along seven biopsy-proven coeliacs. Serum samples tested anti-endomysium antibodies; coeliac-related human...