A5036457471- Electrochemical Analysis and Applications
- Electrochemical sensors and biosensors
- Advanced biosensing and bioanalysis techniques
- Analytical Chemistry and Sensors
- Lipid Membrane Structure and Behavior
- Photoreceptor and optogenetics research
- Biosensors and Analytical Detection
- Neuroscience and Neural Engineering
- Cellular transport and secretion
- Nitric Oxide and Endothelin Effects
- Mitochondrial Function and Pathology
- Plasma Applications and Diagnostics
- Molecular Junctions and Nanostructures
- Advanced Fluorescence Microscopy Techniques
- ATP Synthase and ATPases Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Bioactive Compounds and Antitumor Agents
- Metabolomics and Mass Spectrometry Studies
- bioluminescence and chemiluminescence research
- Conducting polymers and applications
- Ion channel regulation and function
- Micro and Nano Robotics
- Electron Spin Resonance Studies
- Force Microscopy Techniques and Applications
- Advanced Chemical Sensor Technologies
Université de Bordeaux
2015-2024
Institut Polytechnique de Bordeaux
2015-2024
Centre National de la Recherche Scientifique
2015-2024
Chimie et Biologie des Membranes et des Nanoobjects
2022-2024
Institut Européen de Chimie et Biologie
2022-2024
Institut des Sciences Moléculaires
2014-2023
École Nationale Supérieure de Chimie, de Biologie et de Physique
2015-2018
Institut de Mécanique et d'Ingénierie
2017
Canadian Nautical Research Society
2014-2016
Ministère de la Culture
2015
We report here the development of coreactant-based electrogenerated chemiluminescence (ECL) as a surface-confined microscopy to image single cells and their membrane proteins. Labeling entire cell allows one demonstrate that, by contrast with fluorescence, ECL emission is only detected from fluorophores located in immediate vicinity electrode surface (i.e., 1–2 μm). Then, present potential diagnostic applications our approach, we selected carbon nanotubes (CNT)-based inkjet-printed...
Herein is reported a surface-confined microscopy based on electrochemiluminescence (ECL) that allows to image the plasma membrane of single cells at interface with an electrode. By analyzing photoluminescence (PL), ECL and AFM images mammalian CHO cells, we demonstrate that, in contrast wide-field fluorescence, emission confined immediate vicinity electrode surface only basal cell becomes luminescent. The resulting reveals details are not resolved by classic fluorescence microscopy, without...
Mapping the reactivity of a redox-sensitive luminescent microobject positioned in fluxes reactive species allows analyzing complex mechanistic processes such as electrogenerated chemiluminescence model systems used immunoassays.
Here we report on a label-free electrochemiluminescence (ECL) microscopy using exceptionally low concentrations of the [Ru(bpy)3 ]2+ luminophore. This work addresses central point minimal concentration ECL luminophore required to image single entities. We demonstrate possibility record images cells and mitochondria at down nM pM. is 7 orders magnitude lower than classically-used corresponds few hundreds luminophores diffusing around biological Yet, it produces remarkably sharp negative...
Macrophages are key cells of the immune system. During phagocytosis, macrophage engulfs a foreign bacterium, virus, or particle into vacuole, phagosome, wherein oxidants produced to neutralize and decompose threatening element. These derive from in situ production superoxide nitric oxide by specific enzymes. However, chemical nature sequence release these compounds is far being completely determined. The aim present work was study fundamental mechanism oxidant macrophages at level single...
Cold Atmospheric Plasmas (CAPs) are increasingly used for biomedical applications, their various reactive components must be then better determined. We demonstrate that peroxynitrite (ONOO<sup>−</sup>) is effectively a major species generated by CAPs.
Mitochondria are the subcellular bioenergetic organelles. The analysis of their morphology and topology is essential to provide useful information on activity metabolism. Herein, we report a label-free shadow electrochemiluminescence (ECL) microscopy based spatial confinement ECL-emitting reactive layer image single living mitochondria deposited electrode surface. ECL mechanism freely-diffusing [Ru(bpy)3 ]2+ dye with sacrificial tri-n-propylamine coreactant restrains light-emitting region...
The tight coupling between increased neuronal activity and local cerebral blood flow, known as functional hyperemia, is essential for normal brain function. However, its cellular molecular mechanisms remain poorly understood. In the cerebellum, hyperemia depends almost exclusively on nitric oxide (NO). Here, we investigated role of different populations in control microvascular tone by situ amperometric detection NO infrared videomicroscopy microvessel movements rat cerebellar slices. Bath...
Many spikes in amperometric records of exocytosis events initially exhibit a prespike feature, or foot, which represents steady-state flux neurotransmitter through stable fusion pore spanning both the vesicle and plasma membranes connecting lumen to extracellular fluid. Here, we present first evidence indicating that vesicular volume before secretion is strongly correlated with characteristics foot events. l -3,4-Dihydroxyphenylalanine reserpine have been used increase decrease,...
ADVERTISEMENT RETURN TO ISSUEArticleNEXTMonitoring an oxidative stress mechanism at a single human fibroblastStephane. Arbault, Paul. Pantano, Jeffrey A. Jankowski, Monique. Vuillaume, and Christian. AmatoreCite this: Anal. Chem. 1995, 67, 19, 3382–3390Publication Date (Print):October 1, 1995Publication History Published online1 May 2002Published inissue 1 October 1995https://pubs.acs.org/doi/10.1021/ac00115a004https://doi.org/10.1021/ac00115a004research-articleACS PublicationsRequest reuse...
Macrophages produce reactive oxygen and nitrogen species (ROS/RNS) in response to immunological challenges. We have previously reported the real-time detection quantification of released ROS/RNS by immunostimulated macrophages using constant potential amperometry, at four different potentials, with platinized carbon microelectrodes. As a methodological extension that work, we sought develop an electroanalytical method would allow for simultaneous monitoring several ROS/RNS. Triple...
We present herein the combined local enzymatic sensing and dynamic ECL reporting of a glucose concentration gradient explored by bipolar swimmer.
Electrogenerated chemiluminescence is extended to the 3D by generating light at level of millions micro-emitters addressed remotely bipolar electrochemistry.
We report here the design of a novel immunosensor and its application for celiac disease diagnosis, based on an electrogenerated chemiluminescence (ECL) readout, using membrane-templated gold nanoelectrode ensembles (NEEs) as detection platform. An original sensing strategy is presented by segregating spatially initial electrochemical reaction location immobilized biomolecules where ECL finally emitted. The recognition scaffold following: tissue transglutaminase (tTG) capturing agent...
Abstract Compelling evidence suggests that Cold Atmospheric Pressure Plasma (CAPP) has potential as a new cancer therapy. However, knowledge about cellular signaling events and toxicity subsequent to plasma treatment is still poorly documented. The aim of this study was focus on the interaction between 3 different types (He, He-O 2 , He-N ) human epithelial cell lines gain better insight into plasma-cell interaction. We provide reactive oxygen nitrogen species (RONS) are inducing death by...
Understanding the role of gaseous environment in plasma–liquid interactions is crucial for multiple applications CAPs.
The electrochemical signature of peroxynitrite oxidation is reported for the first time, and its mechanism discussed in light data obtained by steady-state transient voltammetry at microelectrodes. Peroxynitrite an important biological species generated aerobic cells presumably via near diffusion-limited coupling nitric oxide superoxide ion. Its production living has been previously suspected during cellular oxidative bursts as well several human pathologies (arthritis, inflammation,...
Macrophages are key cells of the immune system. Immunologically activated macrophages known to release a cocktail reactive oxygen and nitrogen species. In this work, RAW 264.7 were by interferon-gamma lipopolysaccharide, mixture released single was analyzed, in real time, amperometry at platinized carbon microelectrodes. comparison with untreated macrophages, significant increases amperometric responses observed for macrophages. Nitric oxide (NO*), nitrite (NO2*-), peroxynitrite (ONOO-) main...
Lead-based compounds were used during antiquity as both pigments and medicines in the formulation of makeup materials. Chemical analysis cosmetics samples found Egyptians tombs reconstitution ancient recipes reported by Greco-Roman authors have shown that two non-natural lead chlorides (laurionite Pb(OH)Cl phosgenite Pb2Cl2CO3) purposely synthesized fine powders eye lotions. According to Egyptian manuscripts, these essential remedies for treating illness skin ailments. This conclusion seems...
More transparency in bioanalysis: A microdevice based on transparent indium tin oxide (ITO) electrodes allows simultaneous total internal reflection fluorescence microscopy and amperometric measurements. Use of the device coupled optical electrochemical detection single exocytotic events is demonstrated with enterochromaffin BON cells (see picture).