A5008191157- CRISPR and Genetic Engineering
- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- Probiotics and Fermented Foods
- Genomics and Phylogenetic Studies
- Cytomegalovirus and herpesvirus research
- Bacterial Genetics and Biotechnology
- Streptococcal Infections and Treatments
- Innovation and Socioeconomic Development
- Vibrio bacteria research studies
- Microbial infections and disease research
- Advanced biosensing and bioanalysis techniques
- Insect symbiosis and bacterial influences
- Neonatal and Maternal Infections
- Biosensors and Analytical Detection
- RNA regulation and disease
- Microbial Metabolites in Food Biotechnology
- Plant Virus Research Studies
- Peptidase Inhibition and Analysis
- Microbial Metabolic Engineering and Bioproduction
- Evolution and Genetic Dynamics
- Bacterial biofilms and quorum sensing
- Biochemical and Molecular Research
- Chemotherapy-related skin toxicity
- Biochemical and Structural Characterization
DuPont (France)
2012-2021
DuPont (United States)
2012-2013
Vilnius University
2011
Université Laval
2007
Rush University Medical Center
1995
Clustered regularly interspaced short palindromic repeats (CRISPR) are a distinctive feature of the genomes most Bacteria and Archaea thought to be involved in resistance bacteriophages. We found that, after viral challenge, bacteria integrated new spacers derived from phage genomic sequences. Removal or addition particular modified phage-resistance phenotype cell. Thus, CRISPR, together with associated cas genes, provided against phages, specificity is determined by spacer-phage sequence similarity.
Clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems provide adaptive immunity against viruses and plasmids in bacteria archaea. The silencing of invading nucleic acids is executed by ribonucleoprotein complexes preloaded with small, interfering CRISPR RNAs (crRNAs) that act as guides for targeting degradation foreign acid. Here, we demonstrate the Cas9–crRNA complex Streptococcus thermophilus CRISPR3/Cas system introduces vitro a double-strand...
ABSTRACT Clustered regularly interspaced short palindromic repeats (CRISPR) and their associated genes are linked to a mechanism of acquired resistance against bacteriophages. Bacteria can integrate stretches phage-derived sequences (spacers) within CRISPR loci become phage resistant. In this study, we further characterized the efficiency CRISPR1 as in Streptococcus thermophilus . First, show that is distinct from previously known defense systems effective two main groups S. phages. Analyses...
The CRISPR/Cas adaptive immune system provides resistance against phages and plasmids in Archaea Bacteria.CRISPR loci integrate short DNA sequences from invading genetic elements that provide small RNA-mediated interference subsequent exposure to matching nucleic acids.In Streptococcus thermophilus, it was previously shown the CRISPR1/Cas can immunity by integrating novel spacers following these foreign subsequently direct specific cleavage of invasive homologous sequences.Here, we show S....
ABSTRACT Clustered regularly interspaced short palindromic repeats (CRISPR) are hypervariable loci widely distributed in prokaryotes that provide acquired immunity against foreign genetic elements. Here, we characterize a novel Streptococcus thermophilus locus, CRISPR3, and experimentally demonstrate its ability to integrate spacers response bacteriophage. Also, analyze CRISPR diversity activity across three distinct several S. strains. We show both cas genes locus specific functionally...
The Cas9-crRNA complex of the Streptococcus thermophilus DGCC7710 CRISPR3-Cas system functions as an RNA-guided endonuclease with crRNA-directed target sequence recognition and protein-mediated DNA cleavage. We show here that additional RNA molecule, tracrRNA (trans-activating CRISPR RNA), co-purifies Cas9 protein isolated from heterologous E. coli strain carrying S. system. provide experimental evidence is required for Cas9-mediated interference both in vitro vivo. specifically promotes...
Abstract CRISPR-Cas systems are bacterial anti-viral systems, and viruses (bacteriophages, phages) can carry anti-CRISPR (Acr) proteins to evade that immunity. Acrs also fine-tune the activity of CRISPR-based genome-editing tools. While prevalent in phages capable lying dormant a CRISPR-carrying host, their orthologs have been observed only infrequently virulent phages. Here we identify AcrIIA6, an Acr encoded 33% Streptococcus thermophilus phage genomes. The X-ray structure AcrIIA6 displays...
In streptococcal species, the key step of competence development is transcriptional induction comX, which encodes alternative sigma factor sigma(X), positively regulates genes necessary for DNA transformation. Streptococcus species belonging to mitis and mutans groups, comX relies on activation a three-component system consisting secreted pheromone, histidine kinase, response regulator. thermophilus, salivarius group, oligopeptide transporter Ami essential expression under...
Well-studied innate immune systems exist throughout bacteria and archaea, but a more recently discovered genomic locus may offer prokaryotes surprising immunological adaptability. Mediated by cassette-like termed Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR), the microbial adaptive system differs from its eukaryotic analogues incorporating new immunities unidirectionally. CRISPR thus stores genomically recoverable timelines of virus-host coevolution in natural organisms...
The broad ecological distribution of L. casei makes it an insightful subject for research on genome evolution and lifestyle adaptation. To explore evolutionary mechanisms that determine genomic diversity casei, we performed comparative analysis 17 genomes representing strains collected from dairy, plant, human sources. Differences in inventory revealed open pan-genome comprised 1,715 core 4,220 accessory genes. Extrapolation data indicates has a supragenome approximately 3.2 times larger...
ABSTRACT Bifidobacteria are important members of the human gut flora, especially in infants. Comparative genomic analysis two Bifidobacterium animalis subsp. lactis strains revealed evolution by internal deletion consecutive spacer-repeat units within a novel clustered regularly interspaced short palindromic repeat locus, which represented largest differential content between genomes. Additionally, 47 single nucleotide polymorphisms were identified, consisting primarily nonsynonymous...
Interactions between bacteria and their coexisting phage populations impact evolution can strongly influence biogeochemical processes in natural ecosystems. Periodically, mutation or migration results exposure of a host to which it has no immunity; alternatively, may be exposed cannot infect. To explore the by coexisting, co-evolving hosts establish, we cultured Streptococcus thermophilus DGCC7710 with 2972 tracked CRISPR (clustered regularly interspaced short palindromic repeats)...
Abstract CRISPR–Cas systems are defense mechanisms against phages and other nucleic acids that invade bacteria archaea. In Escherichia coli, it is generally accepted inactive in laboratory conditions due to a transcriptional repressor. natural isolates, has been shown CRISPR arrays remain stable over the years most spacer targets (protospacers) unknown. Here, we re-examine E. coli isolates investigate viral bacterial genomes for using bioinformatics approach coupled unique biological...