A5090447233- Animal Disease Management and Epidemiology
- Viral Infections and Immunology Research
- Vector-Borne Animal Diseases
- Animal Virus Infections Studies
- Hepatitis C virus research
- Hepatitis B Virus Studies
- Virus-based gene therapy research
- Viral gastroenteritis research and epidemiology
- HIV Research and Treatment
- Viral Infectious Diseases and Gene Expression in Insects
- Mosquito-borne diseases and control
- Viral Infections and Vectors
- RNA and protein synthesis mechanisms
- Plant Virus Research Studies
- Liver Disease Diagnosis and Treatment
- CRISPR and Genetic Engineering
- Advanced biosensing and bioanalysis techniques
- Biochemical and Molecular Research
- Glycosylation and Glycoproteins Research
- Signaling Pathways in Disease
- SARS-CoV-2 and COVID-19 Research
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Infectious Encephalopathies and Encephalitis
- Insect symbiosis and bacterial influences
- Influenza Virus Research Studies
University of Lübeck
2015-2025
Justus-Liebig-Universität Gießen
1996-2006
Rockefeller University
2006
Leiden University Medical Center
2004
New South Wales Department of Primary Industries
1994
Cornell University
1992
Organic Research Centre
1992
New York State College of Veterinary Medicine
1992
Roche (United States)
1990
The first marine pestivirus, Phocoena pestivirus (PhoPeV), isolated from harbor porpoise, has been recently described. To further characterize this unique its host cell tropism and growth kinetics were determined in different lines. In addition, the interaction of PhoPeV with innate immunity porcine epithelial cells role selected cellular factors involved viral entry RNA replication investigated comparison to closely distantly related pestiviruses. While Bungowannah (BuPV), a PhoPeV,...
ABSTRACT As an initial approach to define the requirements for replication of bovine viral diarrhea virus (BVDV), a member Flaviviridae family with positive-strand RNA genome, full-length genomic and subgenomic RNAs were originated by in vitro transcription diverse BVDV cDNA constructs transfected into eucaryotic host cells. was measured either directly RNase protection method or monitoring synthesis protein. When cRNA initially applied, negative-strand intermediates as well progeny detected...
ABSTRACT The genes encoding pestivirus E2 and NS2-3 are separated by a sequence that encodes small hydrophobic polypeptide with an apparent molecular mass of 6 to 7 kDa (p7). It has been shown cleavage between p7 is incomplete, resulting in proteins E2-p7, E2, p7. We found no precursor-product relationship E2-p7 which indicates stable nature E2-p7. To study the function region polyprotein, mutations were introduced into infectious cDNA bovine viral diarrhea virus (BVDV). When was abolished,...
ABSTRACT Pestiviruses belong to the family Flaviviridae , and their genome is a single-stranded RNA of positive polarity encoding one large polyprotein which further processed into mature proteins. Noncytopathogenic (noncp) strains pestivirus bovine viral diarrhea virus (BVDV) can establish persistent infection. In persistently infected animals, noncp BVDVs occasionally acquire mutations in nonstructural protein 2 (NS2) that give rise cytopathogenic (cp) BVDV variants, and, eventually, lead...
The pestivirus genome encodes a single polyprotein which is subject to co- and posttranslational processing by cellular viral proteases. map positions of all virus-encoded proteins are known with the exception hypothetical peptide (p?) interlinks glycoprotein E2 nonstructural protein NS2-3 approximately between amino acid 1060 1130. Expression studies recombinant vaccinia viruses bearing set C-terminally truncated E2-p?-NS2-encoding sequences derived from bovine diarrhea virus (BVDV) strain...
After cDNA cloning of the genome bovine viral diarrhea virus (BVDV) isolate CP7, a full-length clone was constructed. RNA transcribed in vitro from this construct shown to direct generation infectious BVDV upon transfection into cells. To confirm de novo cloned genetically tagged In comparison with parental BVDV, recombinant slightly retarded growth. The NS2 coding region CP7 contains duplication 27 nucleotides which is not present its noncytopathogenic counterpart, NCP7. Exchange small...
Two cytopathogenic isolates of bovine viral diarrhea virus (cpBVDV) have been analyzed. For both viruses two regions their genomic RNAs were found to be duplicated and rearranged. The genomes contain a small element (SD) derived from the 5' end far downstream its original context. This sequence is followed by larger duplication which encompasses region coding for protein p80(LD), molecular marker cpBVDV. SD codes protease p20. In case analyzed here aminoterminus p80 generated autoproteolytic...
Abstract Numerous anti-hepatitis C virus (HCV) drugs targeting either the viral nonstructural 3 (NS3) protease or NS5B polymerase are currently in clinical testing. However, rapid resistance development is a major problem and optimal therapy will clearly require combination of multiple mechanisms action. Cyclosporine A (CsA) its nonimmunosuppressant derivatives among more promising under development. Based on work with subgenomic HCV replicons it has been thought that they act as...
ABSTRACT The family Flaviviridae consists of four genera, Flavivirus , Pestivirus Pegivirus and Hepacivirus comprises important pathogens human animals. Although the construction recombinant viruses carrying reporter genes encoding fluorescent bioluminescent proteins has been reported, stable insertion foreign into viral genomes retaining infectivity remains difficult. Here, we applied 11-amino-acid subunit derived from NanoLuc luciferase to engineering then examined biological...
Cytopathogenic bovine viral diarrhea virus (BVDV) arises by RNA recombination in animals persistently infected with noncytopathogenic BVDV. Such develop fatal mucosal disease. In this report, the genome of a cytopathogenic BVDV isolate, termed CP9, is characterized. CP9-infected cells contained not only genomic 12.3 kb but also BVDV-specific 8 kb. cDNA cloning and sequencing revealed that 8-kb an internal deletion 4.3 The represents typical defective interfering particle (DI), since its...
The single-stranded genomic RNA of pestiviruses is positive polarity and encompasses one large open reading frame about 4,000 codons. resulting polyprotein processed co- posttranslationally by virus-encoded host cell proteases to give rise the mature viral proteins. A serine protease residing in nonstructural (NS) protein NS3 (p80) has been shown be essential for release NS proteins located downstream NS3. In this report protease-dependent cleavage sites bovine diarrhea virus (BVDV) strain...
Chronic infection with hepatitis C virus (HCV) affects 130 million people worldwide and is a major cause of liver cirrhosis cancer. After translation the HCV RNA genome into polyprotein, 2 viral proteases process its non-structural protein (NS) region. While essential chymotrypsin-like serine protease NS3-4A mediates all cleavages downstream NS3, NS2-3 cysteine catalyzes vital cleavage at NS2/3 site. Protease activity has been described to require, besides NS2, N-terminal 181 aa NS3. The...
Cytopathogenic (cp) bovine viral diarrhea virus (BVDV) strains are generated in cattle persistently infected with noncytopathogenic (noncp) BVDV.cp BVDV considered crucial for the development of fatal mucosal disease. Comparative analysis cp and noncp isolated from one animal suffering disease revealed that genomes strain (CP7) corresponding (NCP7) highly homologous. However, only genome CP7 contains an insertion 27 nucleotides NS2 coding region. The inserted sequence represents a...
Pestiviruses such as bovine viral diarrhea virus (BVDV) and classical swine fever (CSFV) belong to the family Flaviviridae represent pathogens of outstanding veterinary relevance. enter cells via receptor-mediated endocytosis. For entry in cells, complement regulatory protein CD46bov serves a cellular receptor for BVDV. In this study, role porcine CD46pig was investigated recently discovered atypical pestivirus (APPV), CSFV, Bungowannah (BuPV) order elucidate observed differences host cell...
Pestiviruses are positive-strand RNA viruses closely related to human hepatitis C virus. Gene expression of these occurs via translation a polyprotein, which is further processed by cellular and viral proteases. Here we report the formation stable complex between an as-yet-undescribed J-domain protein, member DnaJ-chaperone family, pestiviral nonstructural protein NS2. Accordingly, termed Jiv, for interacting with protein. Jiv has potential induce in trans one specific processing step...
ABSTRACT The functional analysis of molecular determinants which control the replication pestiviruses was considerably facilitated by finding that subgenomic forms positive-strand RNA genome BVDV (bovine viral diarrhea virus) are capable autonomous in transfected host cells. prototype replicon, DI9c, consists genomic 5′ and 3′ untranslated regions a truncated open reading frame (ORF) encoding mainly nonstructural proteins NS3, NS4A, NS4B, NS5A, NS5B. To gain insight into these essential for...
We have used chemical shift perturbation (CSP) and saturation transfer difference (STD) NMR experiments to identify characterize the binding of selected ligands receptor-binding domain (RBD) spike glycoprotein (S-protein) severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). also subjected full-length S-protein STD experiments, allowing correlations with RBD-based results. CSPs reveal sites for heparin fondaparinux, affinities were measured using CSP titrations. then show that...
ABSTRACT Defective interfering particles (DIs) of bovine viral diarrhea virus (BVDV) have been identified and shown to be cytopathogenic (cp) in the presence noncytopathogenic (noncp) helper virus. Moreover, a subgenomic (sg) RNA corresponding its genome structure one those BVDV DIs (DI9) was replication competent absence We report here that an sg replicon which encodes from proteins only first three amino acids autoprotease N pro addition nonstructural (NS) NS3 NS5B replicates autonomously...